Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress
Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low tem...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Molecular Diversity Preservation International
2020
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/23309/1/23309.pdf |
| _version_ | 1824416834752872448 |
|---|---|
| author | Acosta Pérez, Phamela Camacho Zamora, Bianka Dianey Espinoza Sánchez, Edward Alexander Gutiérrez Soto, Juanita Guadalupe Zavala García, Francisco Abraham Juárez, María Jazmín Sinagawa García, Sugey Ramona |
| author_facet | Acosta Pérez, Phamela Camacho Zamora, Bianka Dianey Espinoza Sánchez, Edward Alexander Gutiérrez Soto, Juanita Guadalupe Zavala García, Francisco Abraham Juárez, María Jazmín Sinagawa García, Sugey Ramona |
| author_sort | Acosta Pérez, Phamela |
| collection | Repositorio Institucional |
| description | Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low temperature, and osmotic stress. Thus, the aim of this work was to clone and characterize at molecular level the trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes from maize and to evaluate its differential expression in maize seedlings under drought stress. To carry out this, resistant and susceptible maize lines were subjected to drought stress during 72 h. Two full-length cDNAs of TPS and one of TPP were cloned and sequenced. Then, TPS and TPP amino acid sequences were aligned with their homologs from different species, showing highly conserved domains and the same catalytic sites. Relative expression of both genes was evaluated by RT-qPCR at different time points. The expression pattern showed significant induction after 0.5 h in resistant lines and after two to four hours in susceptible plants, showing their participation in drought stress response. |
| format | Article |
| id | eprints-23309 |
| institution | UANL |
| language | English |
| publishDate | 2020 |
| publisher | Molecular Diversity Preservation International |
| record_format | eprints |
| spelling | eprints-233092024-03-05T20:49:58Z http://eprints.uanl.mx/23309/ Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress Acosta Pérez, Phamela Camacho Zamora, Bianka Dianey Espinoza Sánchez, Edward Alexander Gutiérrez Soto, Juanita Guadalupe Zavala García, Francisco Abraham Juárez, María Jazmín Sinagawa García, Sugey Ramona QK Botánica Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low temperature, and osmotic stress. Thus, the aim of this work was to clone and characterize at molecular level the trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes from maize and to evaluate its differential expression in maize seedlings under drought stress. To carry out this, resistant and susceptible maize lines were subjected to drought stress during 72 h. Two full-length cDNAs of TPS and one of TPP were cloned and sequenced. Then, TPS and TPP amino acid sequences were aligned with their homologs from different species, showing highly conserved domains and the same catalytic sites. Relative expression of both genes was evaluated by RT-qPCR at different time points. The expression pattern showed significant induction after 0.5 h in resistant lines and after two to four hours in susceptible plants, showing their participation in drought stress response. Molecular Diversity Preservation International 2020 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23309/1/23309.pdf http://eprints.uanl.mx/23309/1.haspreviewThumbnailVersion/23309.pdf Acosta Pérez, Phamela y Camacho Zamora, Bianka Dianey y Espinoza Sánchez, Edward Alexander y Gutiérrez Soto, Juanita Guadalupe y Zavala García, Francisco y Abraham Juárez, María Jazmín y Sinagawa García, Sugey Ramona (2020) Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress. Plants, 9 (3). pp. 1-12. ISSN 2223-7747 http://doi.org/10.3390/plants9030315 doi:10.3390/plants9030315 |
| spellingShingle | QK Botánica Acosta Pérez, Phamela Camacho Zamora, Bianka Dianey Espinoza Sánchez, Edward Alexander Gutiérrez Soto, Juanita Guadalupe Zavala García, Francisco Abraham Juárez, María Jazmín Sinagawa García, Sugey Ramona Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| title_full | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| title_fullStr | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| title_full_unstemmed | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| title_short | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
| title_sort | characterization of trehalose 6 phosphate synthase and trehalose 6 phosphate phosphatase genes and analysis of its differential expression in maize zea mays seedlings under drought stress |
| topic | QK Botánica |
| url | http://eprints.uanl.mx/23309/1/23309.pdf |
| work_keys_str_mv | AT acostaperezphamela characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT camachozamorabiankadianey characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT espinozasanchezedwardalexander characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT gutierrezsotojuanitaguadalupe characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT zavalagarciafrancisco characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT abrahamjuarezmariajazmin characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress AT sinagawagarciasugeyramona characterizationoftrehalose6phosphatesynthaseandtrehalose6phosphatephosphatasegenesandanalysisofitsdifferentialexpressioninmaizezeamaysseedlingsunderdroughtstress |