Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way
Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and durati...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sage Publishing
2022
|
| Subjects: | |
| Online Access: | http://eprints.uanl.mx/23293/1/23293.pdf |
| _version_ | 1824416829796253696 |
|---|---|
| author | García Cuellar, Claudia María Hernández Delgadillo, René Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Nakagoshi Cepeda, Sergio Eduardo Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_facet | García Cuellar, Claudia María Hernández Delgadillo, René Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Nakagoshi Cepeda, Sergio Eduardo Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_sort | García Cuellar, Claudia María |
| collection | Repositorio Institucional |
| description | Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity. |
| format | Article |
| id | eprints-23293 |
| institution | UANL |
| language | English |
| publishDate | 2022 |
| publisher | Sage Publishing |
| record_format | eprints |
| spelling | eprints-232932022-05-25T20:15:41Z http://eprints.uanl.mx/23293/ Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way García Cuellar, Claudia María Hernández Delgadillo, René Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Nakagoshi Cepeda, Sergio Eduardo Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio RC Medicina Interna, Psiquiatría, Neurología Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity. Sage Publishing 2022 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/23293/1/23293.pdf http://eprints.uanl.mx/23293/1.haspreviewThumbnailVersion/23293.pdf García Cuellar, Claudia María y Hernández Delgadillo, René y Solís Soto, Juan Manuel y Meester, Irene y Sánchez Pérez, Yesennia y Nakagoshi Cepeda, Sergio Eduardo y Nakagoshi Cepeda, María Argelia Akemi y Chellam, Shankararaman y Cabral Romero, Claudio (2022) Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way. Journal of Applied Biomaterials & Functional Materials, 20. pp. 1-9. ISSN 2280-8000 http://doi.org/10.1177/22808000221092157 doi:10.1177/22808000221092157 |
| spellingShingle | RC Medicina Interna, Psiquiatría, Neurología García Cuellar, Claudia María Hernández Delgadillo, René Solís Soto, Juan Manuel Meester, Irene Sánchez Pérez, Yesennia Nakagoshi Cepeda, Sergio Eduardo Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| title_full | Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| title_fullStr | Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| title_full_unstemmed | Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| title_short | Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way |
| title_sort | cetylpyridinium chloride inhibits human breast tumor cells growth in a no selective way |
| topic | RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/23293/1/23293.pdf |
| work_keys_str_mv | AT garciacuellarclaudiamaria cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT hernandezdelgadillorene cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT solissotojuanmanuel cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT meesterirene cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT sanchezperezyesennia cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT nakagoshicepedasergioeduardo cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT nakagoshicepedamariaargeliaakemi cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT chellamshankararaman cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway AT cabralromeroclaudio cetylpyridiniumchlorideinhibitshumanbreasttumorcellsgrowthinanoselectiveway |