Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.

The presence in the New World of a variant strain of Plasmodium vivax (VK247) containing a unique circumsporozoite (CS) repeat domain was determined by the detection of antibodies to the variant CS protein and by genetic analysis of the CS gene from field isolates. Whole blood specimens were collect...

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Autores principales: Kain, Kevin C., Wirtz, Robert A., Fernández Salas, Ildefonso, Franke, Eileen D., Rodríguez, Mario Henry, Lanar, David
Formato: Artículo
Lenguaje:inglés
Publicado: The American journal of tropical medicine and hygiene. 1992
Acceso en línea:http://eprints.uanl.mx/224/1/Serologic_and_genetic_characterization_of_Plasmodium_vivax_from_whole_bloog_impregnated_filter_paper_discs.pdf
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author Kain, Kevin C.
Wirtz, Robert A.
Fernández Salas, Ildefonso
Franke, Eileen D.
Rodríguez, Mario Henry
Lanar, David
author_facet Kain, Kevin C.
Wirtz, Robert A.
Fernández Salas, Ildefonso
Franke, Eileen D.
Rodríguez, Mario Henry
Lanar, David
author_sort Kain, Kevin C.
collection Repositorio Institucional
description The presence in the New World of a variant strain of Plasmodium vivax (VK247) containing a unique circumsporozoite (CS) repeat domain was determined by the detection of antibodies to the variant CS protein and by genetic analysis of the CS gene from field isolates. Whole blood specimens were collected on filter paper from patients infected with P. vivax in Mexico and Peru. Plasmodium vivax DNA was eluted from filter paper samples and the CS gene was amplified by the polymerase chain reaction (PCR) and analyzed for the presence of VK247 or VK210 DNA by oligoprobe hybridization. Sera eluted from a companion filter paper sample were screened for antibodies reactive with the predominant and variant repeat peptides by enzyme-linked immunosorbent assays (ELISA) and with sporozoites by the immunofluorescent antibody (IFA) test. All 24 patients were positive by PCR and oligoprobe hybridization for either VK210 (16 of 24), VK247 (3 of 24), or both (5 of 24). Mixed infections were common (5 of 7) in Peru, but were not observed in the Mexican isolates (0 of 17). All three VK247 infections from Mexico occurred in residents of the foothills above Tapachula (P = 0.02). Of patients with smear-positive P. vivax infection, 42% (10 of 24) had detectable antibodies eluted from dried blood dots that were reactive with the CS protein by IFA or ELISA. These findings establish the widespread distribution of the P. vivax variant CS protein in the New World and indicate that dried blood filter paper samples represent a valuable source of material for the serologic and molecular analysis of plasmodial infections.
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spelling eprints-2242015-01-19T22:28:48Z http://eprints.uanl.mx/224/ Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs. Kain, Kevin C. Wirtz, Robert A. Fernández Salas, Ildefonso Franke, Eileen D. Rodríguez, Mario Henry Lanar, David The presence in the New World of a variant strain of Plasmodium vivax (VK247) containing a unique circumsporozoite (CS) repeat domain was determined by the detection of antibodies to the variant CS protein and by genetic analysis of the CS gene from field isolates. Whole blood specimens were collected on filter paper from patients infected with P. vivax in Mexico and Peru. Plasmodium vivax DNA was eluted from filter paper samples and the CS gene was amplified by the polymerase chain reaction (PCR) and analyzed for the presence of VK247 or VK210 DNA by oligoprobe hybridization. Sera eluted from a companion filter paper sample were screened for antibodies reactive with the predominant and variant repeat peptides by enzyme-linked immunosorbent assays (ELISA) and with sporozoites by the immunofluorescent antibody (IFA) test. All 24 patients were positive by PCR and oligoprobe hybridization for either VK210 (16 of 24), VK247 (3 of 24), or both (5 of 24). Mixed infections were common (5 of 7) in Peru, but were not observed in the Mexican isolates (0 of 17). All three VK247 infections from Mexico occurred in residents of the foothills above Tapachula (P = 0.02). Of patients with smear-positive P. vivax infection, 42% (10 of 24) had detectable antibodies eluted from dried blood dots that were reactive with the CS protein by IFA or ELISA. These findings establish the widespread distribution of the P. vivax variant CS protein in the New World and indicate that dried blood filter paper samples represent a valuable source of material for the serologic and molecular analysis of plasmodial infections. The American journal of tropical medicine and hygiene. 1992 Article PeerReviewed application/pdf en http://eprints.uanl.mx/224/1/Serologic_and_genetic_characterization_of_Plasmodium_vivax_from_whole_bloog_impregnated_filter_paper_discs.pdf http://eprints.uanl.mx/224/1.haspreviewThumbnailVersion/Serologic_and_genetic_characterization_of_Plasmodium_vivax_from_whole_bloog_impregnated_filter_paper_discs.pdf Kain, Kevin C. y Wirtz, Robert A. y Fernández Salas, Ildefonso y Franke, Eileen D. y Rodríguez, Mario Henry y Lanar, David (1992) Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs. American journal of tropical medicine and hygiene, 46 (4). pp. 473-479. ISSN 0002-9637
spellingShingle Kain, Kevin C.
Wirtz, Robert A.
Fernández Salas, Ildefonso
Franke, Eileen D.
Rodríguez, Mario Henry
Lanar, David
Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
title_full Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
title_fullStr Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
title_full_unstemmed Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
title_short Serologic and genetic characterization of Plasmodium vivax from whole blood-impregnated filter paper discs.
title_sort serologic and genetic characterization of plasmodium vivax from whole blood impregnated filter paper discs
url http://eprints.uanl.mx/224/1/Serologic_and_genetic_characterization_of_Plasmodium_vivax_from_whole_bloog_impregnated_filter_paper_discs.pdf
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