Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity
Cystic fibrosis (CF) is a disease characterized by bacterial chronic infection of the respiratory tract and inflammation, which leads to a progressive decrease in lung function. Pseudomonas aeruginosa is commonly isolated from the sputum of patients and their presence is associated with a predomin...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2020
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/20121/1/1-s2.0-S1438422120300618-main.pdf |
| _version_ | 1824415756935233536 |
|---|---|
| author | Martínez Alemán, Saira Rocío Bustamante, A.E. Jimenez Valdes, R.J. González González, Gloria María Sánchez González, A. |
| author_facet | Martínez Alemán, Saira Rocío Bustamante, A.E. Jimenez Valdes, R.J. González González, Gloria María Sánchez González, A. |
| author_sort | Martínez Alemán, Saira Rocío |
| collection | Repositorio Institucional |
| description | Cystic fibrosis (CF) is a disease characterized by bacterial chronic infection of the respiratory tract and
inflammation, which leads to a progressive decrease in lung function. Pseudomonas aeruginosa is commonly
isolated from the sputum of patients and their presence is associated with a predominant airway inflammation
with neutrophils, causing chronic colonization and higher mortality rates. Neutrophil extracellular traps (NETs)
have been observed in response against Pseudomonas, however, these cannot eliminate the pathogen from the
respiratory tract, so one possibility is that the bacteria could promote their production to use them as a scaffold to
colonize the lungs and as a nutrient source, however, their overproduction could also lead to increased damage to
the lungs. In this work, we evaluated NETs formation by Pseudomonas clinical isolates obtained from CF patients
and found that these induced NETs formation with globular or spread morphologies, of note, we found that there
is a trend by which the spread forms were induced mainly by isolates obtained from patients with severe disease,
whereas, the globular morphologies were observed for isolates obtained from patients with mild/moderate
disease. Finally, we screened for bacterial molecules implicated in NETs formation and found that Exotoxin S,
pyocin S2 and pyoverdine could participate in the process. |
| format | Article |
| id | eprints-20121 |
| institution | UANL |
| language | English |
| publishDate | 2020 |
| record_format | eprints |
| spelling | eprints-201212024-03-05T17:53:53Z http://eprints.uanl.mx/20121/ Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity Martínez Alemán, Saira Rocío Bustamante, A.E. Jimenez Valdes, R.J. González González, Gloria María Sánchez González, A. QR Microbiología Cystic fibrosis (CF) is a disease characterized by bacterial chronic infection of the respiratory tract and inflammation, which leads to a progressive decrease in lung function. Pseudomonas aeruginosa is commonly isolated from the sputum of patients and their presence is associated with a predominant airway inflammation with neutrophils, causing chronic colonization and higher mortality rates. Neutrophil extracellular traps (NETs) have been observed in response against Pseudomonas, however, these cannot eliminate the pathogen from the respiratory tract, so one possibility is that the bacteria could promote their production to use them as a scaffold to colonize the lungs and as a nutrient source, however, their overproduction could also lead to increased damage to the lungs. In this work, we evaluated NETs formation by Pseudomonas clinical isolates obtained from CF patients and found that these induced NETs formation with globular or spread morphologies, of note, we found that there is a trend by which the spread forms were induced mainly by isolates obtained from patients with severe disease, whereas, the globular morphologies were observed for isolates obtained from patients with mild/moderate disease. Finally, we screened for bacterial molecules implicated in NETs formation and found that Exotoxin S, pyocin S2 and pyoverdine could participate in the process. 2020 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/20121/1/1-s2.0-S1438422120300618-main.pdf http://eprints.uanl.mx/20121/1.haspreviewThumbnailVersion/1-s2.0-S1438422120300618-main.pdf Martínez Alemán, Saira Rocío y Bustamante, A.E. y Jimenez Valdes, R.J. y González González, Gloria María y Sánchez González, A. (2020) Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity. International Journal of Medical Microbiology, 310 (7). p. 151451. ISSN 1438-4221 http://doi.org/10.1016/J.IJMM.2020.151451 doi:10.1016/J.IJMM.2020.151451 |
| spellingShingle | QR Microbiología Martínez Alemán, Saira Rocío Bustamante, A.E. Jimenez Valdes, R.J. González González, Gloria María Sánchez González, A. Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| title_full | Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| title_fullStr | Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| title_full_unstemmed | Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| title_short | Pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| title_sort | pseudomonas aeruginosa isolates from cystic fibrosis patients induce neutrophil extracellular traps with different morphologies that could correlate with their disease severity |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/20121/1/1-s2.0-S1438422120300618-main.pdf |
| work_keys_str_mv | AT martinezalemansairarocio pseudomonasaeruginosaisolatesfromcysticfibrosispatientsinduceneutrophilextracellulartrapswithdifferentmorphologiesthatcouldcorrelatewiththeirdiseaseseverity AT bustamanteae pseudomonasaeruginosaisolatesfromcysticfibrosispatientsinduceneutrophilextracellulartrapswithdifferentmorphologiesthatcouldcorrelatewiththeirdiseaseseverity AT jimenezvaldesrj pseudomonasaeruginosaisolatesfromcysticfibrosispatientsinduceneutrophilextracellulartrapswithdifferentmorphologiesthatcouldcorrelatewiththeirdiseaseseverity AT gonzalezgonzalezgloriamaria pseudomonasaeruginosaisolatesfromcysticfibrosispatientsinduceneutrophilextracellulartrapswithdifferentmorphologiesthatcouldcorrelatewiththeirdiseaseseverity AT sanchezgonzaleza pseudomonasaeruginosaisolatesfromcysticfibrosispatientsinduceneutrophilextracellulartrapswithdifferentmorphologiesthatcouldcorrelatewiththeirdiseaseseverity |