Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves
Cissus incisa is used in traditional Mexican medicine to treat certain ailments, infectious or cancerous diseases. Excepting for our previous research, this species had no scientific reports validating its traditional use. In this study, we evaluated the antibacterial and cytotoxic properties of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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2020
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| Online Access: | http://eprints.uanl.mx/20113/1/1-s2.0-S2405844020315152-main.pdf |
| _version_ | 1824415753498001408 |
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| author | Nocedo Mena, Deyani Rivas Galindo, Verónica Mayela Navarro, Patricia Garza González, Elvira González Maya, Leticia Ríos, María Yolanda García, Abraham Avalos Alanís, Francisco G. Rodríguez Rodríguez, José Camacho Corona, María del Rayo |
| author_facet | Nocedo Mena, Deyani Rivas Galindo, Verónica Mayela Navarro, Patricia Garza González, Elvira González Maya, Leticia Ríos, María Yolanda García, Abraham Avalos Alanís, Francisco G. Rodríguez Rodríguez, José Camacho Corona, María del Rayo |
| author_sort | Nocedo Mena, Deyani |
| collection | Repositorio Institucional |
| description | Cissus incisa is used in traditional Mexican medicine to treat certain ailments, infectious or cancerous diseases.
Excepting for our previous research, this species had no scientific reports validating its traditional use. In this
study, we evaluated the antibacterial and cytotoxic properties of the sphingolipids and others phytocompounds
isolated from C. incisa leaves to increase the scientific knowledge of the Mexican flora. The antibacterial activity
was evaluated against Gram-positive and Gram-negative bacteria by the Microdilution method. Meanwhile, the
cytotoxic potential was determined on six human cancer cells: PC3, Hep3B, HepG2, MCF7, A549, and HeLa; using
an aqueous solution cell proliferation assay kit. A cell line of immortalized human hepatocytes (IHH) was included
as a control of non-cancerous cells. Selectivity index (SI) was determined only against the hepatocellular carcinoma
cell lines. The phytochemical investigation of C. incisa leaves resulted in the isolation and characterization
of five compounds: 2-(20-hydroxydecanoyl amino)-1,3,4-hexadecanotriol-8-ene (1), 2,3-dihydroxypropyl tetracosanoate
(2), β-sitosterol-D-glucopyranoside (3), α-amyrin-3-O-β-D-glucopyranoside (4), and a mixture of cerebrosides
(5). Until now, this is the first report of the sphingolipids (1), (5-IV) and (5-V). Only the compound (4)
and cerebrosides (5) exhibited antibacterial activity reaching a MIC value of 100 μg/mL against Pseudomonas
aeruginosa resistant to carbapenems. While, the acetylated derivate of (3), compound (3Ac) showed the best cytotoxic result against PC3 (IC50 ¼ 43 � 4 μg/mL) and Hep3B (IC50 ¼ 49.0 � 4 μg/mL) cancer cell lines.
Likewise, (3Ac) achieved better SI values on HepG2 and Hep3B cell lines. This research reveals the importance of
study medicinal plants, to identify bioactive molecules as sources of potential drugs. The presence of these
compounds allows us to justify the use of this plant in traditional Mexican medicine. |
| format | Article |
| id | eprints-20113 |
| institution | UANL |
| language | English |
| publishDate | 2020 |
| record_format | eprints |
| spelling | eprints-201132024-03-07T17:46:22Z http://eprints.uanl.mx/20113/ Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves Nocedo Mena, Deyani Rivas Galindo, Verónica Mayela Navarro, Patricia Garza González, Elvira González Maya, Leticia Ríos, María Yolanda García, Abraham Avalos Alanís, Francisco G. Rodríguez Rodríguez, José Camacho Corona, María del Rayo QD Química Cissus incisa is used in traditional Mexican medicine to treat certain ailments, infectious or cancerous diseases. Excepting for our previous research, this species had no scientific reports validating its traditional use. In this study, we evaluated the antibacterial and cytotoxic properties of the sphingolipids and others phytocompounds isolated from C. incisa leaves to increase the scientific knowledge of the Mexican flora. The antibacterial activity was evaluated against Gram-positive and Gram-negative bacteria by the Microdilution method. Meanwhile, the cytotoxic potential was determined on six human cancer cells: PC3, Hep3B, HepG2, MCF7, A549, and HeLa; using an aqueous solution cell proliferation assay kit. A cell line of immortalized human hepatocytes (IHH) was included as a control of non-cancerous cells. Selectivity index (SI) was determined only against the hepatocellular carcinoma cell lines. The phytochemical investigation of C. incisa leaves resulted in the isolation and characterization of five compounds: 2-(20-hydroxydecanoyl amino)-1,3,4-hexadecanotriol-8-ene (1), 2,3-dihydroxypropyl tetracosanoate (2), β-sitosterol-D-glucopyranoside (3), α-amyrin-3-O-β-D-glucopyranoside (4), and a mixture of cerebrosides (5). Until now, this is the first report of the sphingolipids (1), (5-IV) and (5-V). Only the compound (4) and cerebrosides (5) exhibited antibacterial activity reaching a MIC value of 100 μg/mL against Pseudomonas aeruginosa resistant to carbapenems. While, the acetylated derivate of (3), compound (3Ac) showed the best cytotoxic result against PC3 (IC50 ¼ 43 � 4 μg/mL) and Hep3B (IC50 ¼ 49.0 � 4 μg/mL) cancer cell lines. Likewise, (3Ac) achieved better SI values on HepG2 and Hep3B cell lines. This research reveals the importance of study medicinal plants, to identify bioactive molecules as sources of potential drugs. The presence of these compounds allows us to justify the use of this plant in traditional Mexican medicine. 2020 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/20113/1/1-s2.0-S2405844020315152-main.pdf http://eprints.uanl.mx/20113/1.haspreviewThumbnailVersion/1-s2.0-S2405844020315152-main.pdf Nocedo Mena, Deyani y Rivas Galindo, Verónica Mayela y Navarro, Patricia y Garza González, Elvira y González Maya, Leticia y Ríos, María Yolanda y García, Abraham y Avalos Alanís, Francisco G. y Rodríguez Rodríguez, José y Camacho Corona, María del Rayo (2020) Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves. Heliyon, 6 (8). e04671. ISSN 2405-8440 http://doi.org/10.1016/J.HELIYON.2020.E04671 doi:10.1016/J.HELIYON.2020.E04671 |
| spellingShingle | QD Química Nocedo Mena, Deyani Rivas Galindo, Verónica Mayela Navarro, Patricia Garza González, Elvira González Maya, Leticia Ríos, María Yolanda García, Abraham Avalos Alanís, Francisco G. Rodríguez Rodríguez, José Camacho Corona, María del Rayo Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| title_full | Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| title_fullStr | Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| title_full_unstemmed | Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| title_short | Antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from Cissus incisa leaves |
| title_sort | antibacterial and cytotoxic activities of new sphingolipids and other constituents isolated from cissus incisa leaves |
| topic | QD Química |
| url | http://eprints.uanl.mx/20113/1/1-s2.0-S2405844020315152-main.pdf |
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