Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model
Background Non-human primate (NHP) diabetic models using chemical ablation of b-cells with STZ have been achieved by several research groups. Chemotherapeutic STZ could lead to serious adverse events including nephrotoxicity, hepatotoxicity, and mortality. Methods We implemented a comprehensive t...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/18861/1/3.pdf |
| _version_ | 1824415368533245952 |
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| author | Frost, Patrice A. Chen, Shuyuan Mezzles, Marguerite J. Voruganti, Venkata Saroja Nava González, Edna Judith Arriaga Cazares, Hector E. Freed, Katy A. Comuzzie, Anthony G. DeFronzo, Ralph A. Kent, Jack W. Grayburn, Paul A. Bastarrachea, Raúl A. |
| author_facet | Frost, Patrice A. Chen, Shuyuan Mezzles, Marguerite J. Voruganti, Venkata Saroja Nava González, Edna Judith Arriaga Cazares, Hector E. Freed, Katy A. Comuzzie, Anthony G. DeFronzo, Ralph A. Kent, Jack W. Grayburn, Paul A. Bastarrachea, Raúl A. |
| author_sort | Frost, Patrice A. |
| collection | Repositorio Institucional |
| description | Background Non-human primate (NHP) diabetic models using chemical
ablation of b-cells with STZ have been achieved by several research groups.
Chemotherapeutic STZ could lead to serious adverse events including nephrotoxicity, hepatotoxicity, and mortality.
Methods We implemented a comprehensive therapeutic strategy that
included the tether system, permanent indwelling catheter implants, an
aggressive hydration protocol, management for pain with IV nubain and
anxiety with IV midazolam, moment-by-moment monitoring of glucose levels post-STZ administration, and continuous intravenous insulin therapy.
Results A triphasic response in blood glucose after STZ administration was
fully characterized. A dangerous hypoglycemic phase was also detected in all
baboons. Other significant findings were hyperglycemia associated with low
levels of plasma leptin, insulin and C-peptide concentrations, hyperglucagonemia, and elevated non-esterified fatty acids (NEFA) concentrations.
Conclusions We successfully induced frank diabetes by IV administering a
single dose of pharmaceutical-grade STZ safely and without adverse events
in conscious tethered baboons. |
| format | Article |
| id | eprints-18861 |
| institution | UANL |
| language | English |
| publishDate | 2015 |
| record_format | eprints |
| spelling | eprints-188612020-05-01T16:04:49Z http://eprints.uanl.mx/18861/ Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model Frost, Patrice A. Chen, Shuyuan Mezzles, Marguerite J. Voruganti, Venkata Saroja Nava González, Edna Judith Arriaga Cazares, Hector E. Freed, Katy A. Comuzzie, Anthony G. DeFronzo, Ralph A. Kent, Jack W. Grayburn, Paul A. Bastarrachea, Raúl A. RA Aspectos Públicos de la Medicina Background Non-human primate (NHP) diabetic models using chemical ablation of b-cells with STZ have been achieved by several research groups. Chemotherapeutic STZ could lead to serious adverse events including nephrotoxicity, hepatotoxicity, and mortality. Methods We implemented a comprehensive therapeutic strategy that included the tether system, permanent indwelling catheter implants, an aggressive hydration protocol, management for pain with IV nubain and anxiety with IV midazolam, moment-by-moment monitoring of glucose levels post-STZ administration, and continuous intravenous insulin therapy. Results A triphasic response in blood glucose after STZ administration was fully characterized. A dangerous hypoglycemic phase was also detected in all baboons. Other significant findings were hyperglycemia associated with low levels of plasma leptin, insulin and C-peptide concentrations, hyperglucagonemia, and elevated non-esterified fatty acids (NEFA) concentrations. Conclusions We successfully induced frank diabetes by IV administering a single dose of pharmaceutical-grade STZ safely and without adverse events in conscious tethered baboons. 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18861/1/3.pdf http://eprints.uanl.mx/18861/1.haspreviewThumbnailVersion/3.pdf Frost, Patrice A. y Chen, Shuyuan y Mezzles, Marguerite J. y Voruganti, Venkata Saroja y Nava González, Edna Judith y Arriaga Cazares, Hector E. y Freed, Katy A. y Comuzzie, Anthony G. y DeFronzo, Ralph A. y Kent, Jack W. y Grayburn, Paul A. y Bastarrachea, Raúl A. (2015) Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model. Journal of Medical Primatology, 44 (4). pp. 202-217. ISSN 00472565 http://doi.org/10.1111/jmp.12182 doi:10.1111/jmp.12182 |
| spellingShingle | RA Aspectos Públicos de la Medicina Frost, Patrice A. Chen, Shuyuan Mezzles, Marguerite J. Voruganti, Venkata Saroja Nava González, Edna Judith Arriaga Cazares, Hector E. Freed, Katy A. Comuzzie, Anthony G. DeFronzo, Ralph A. Kent, Jack W. Grayburn, Paul A. Bastarrachea, Raúl A. Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| title_full | Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| title_fullStr | Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| title_full_unstemmed | Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| title_short | Successful pharmaceutical-grade streptozotocin (STZ)-induced hyperglycemia in a conscious tethered baboon (Papio hamadryas) model |
| title_sort | successful pharmaceutical grade streptozotocin stz induced hyperglycemia in a conscious tethered baboon papio hamadryas model |
| topic | RA Aspectos Públicos de la Medicina |
| url | http://eprints.uanl.mx/18861/1/3.pdf |
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