Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing
ABSTRACT Prebiotics are selectively fermentable dietary compounds that result in changes in the composition and/or activity of the intestinal microbiota, thus conferring benefits upon host health. In veterinary medicine, commercially available products containing prebiotics have not been well studie...
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| Format: | Article |
| Language: | English |
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2017
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| Online Access: | http://eprints.uanl.mx/18262/1/551.pdf |
| _version_ | 1824415189706997760 |
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| author | García Mazcorro, José Francisco Bárcenas Walls, José R. Suchodolski, Jan S. Steiner, Jörg M. |
| author_facet | García Mazcorro, José Francisco Bárcenas Walls, José R. Suchodolski, Jan S. Steiner, Jörg M. |
| author_sort | García Mazcorro, José Francisco |
| collection | Repositorio Institucional |
| description | ABSTRACT Prebiotics are selectively fermentable dietary compounds that result in changes in the composition and/or activity of the intestinal microbiota, thus conferring benefits upon host health. In veterinary medicine, commercially available products containing prebiotics have not been well studied with regard to the changes they trigger on the composition of the gut microbiota. This study evaluated the effect of a commercially available nutraceutical containing fructo-oligosaccharides (FOS) and inulin on the fecal microbiota of healthy cats and dogs when administered for 16 days. Fecal samples were collected at two time points before and at two time points during prebioticadministration. Totalgenomic DNA was obtained from fecal samples and 454 pyrosequencing was used for 16Sr RNA genebacterial profiling. The linear discriminant analysis (LDA) effectsize (LEfSe) method was used for detecting bacterial taxathatmay respond (i.e., increase or decrease in itsrelative abundance) to prebiotic administration. Prebiotic administration was associated with a good acceptance and no side effects (e.g., diarrhea) were reported by the owners. Alowdose of prebiotics (50 mL total regardless of body weight with the end product containing 0.45% of prebiotics) revealed a lower abundanceofGammaproteobacteriaandahigherabundanceofVeillonellaceaeduring prebiotic administration in cats, while Staphylococcaceae showed a higher abundance during prebiotic administration in dogs. These differences were not sufficient to separate bacterial communities as shown by analysis of weighted Uni Fracdistan cemetrics. A
predictiveapproachofthefecalbacterialmetagenomeusingPhylogeneticInvestigation ofCommunitiesbyReconstructionofUnobservedStates(PICRUSt)alsodidnotreveal differences between the period before and during prebiotic administration. A second trial using a higher dose of prebiotics (3.2 mL/kg body weight with the end product containing 3.1% of prebiotics) was tested in dogs and revealed a lower abundance of Dorea (family Clostridiaceae) and a higher abundance of Megamonas and other (unknown) members of Veillonellaceae during prebiotic administration. Again, these changes were not sufficient to separate bacterial communities or predicted metabolic profiles according to treatment. A closer analysis of bacterial communities at all timepoints revealed highly individualized patterns of variation. This study shows a high interindividual variation of fecal bacterial communities from pet cats and dogs, that these communities are relatively stable over time, and that some of this variation can be attributable to prebiotic administration, a phenomenon that may be affected by the amount of the prebiotic administered in the formulation. This study also provides insights into the response of gut bacterial communities in pet cats and dogs during administrationofcommerciallyavailableproductscontainingprebiotics.Morestudies are needed to explore potentially beneficial effects on host health beyond changes in bacterial communities.
Subjects Bioinformatics,Microbiology,VeterinaryMedicine,GastroenterologyandHepatology, Nutrition
Keywords Microbiota,Prebiotics,Health,16SrRNAgene,Fructo-oligosaccharides,Inulin, Feline, Canine, Veterinary medicine, Gastrointestinal health |
| format | Article |
| id | eprints-18262 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-182622024-12-10T19:36:42Z http://eprints.uanl.mx/18262/ Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing García Mazcorro, José Francisco Bárcenas Walls, José R. Suchodolski, Jan S. Steiner, Jörg M. SF Ganadería / Medicina veretrinaria ABSTRACT Prebiotics are selectively fermentable dietary compounds that result in changes in the composition and/or activity of the intestinal microbiota, thus conferring benefits upon host health. In veterinary medicine, commercially available products containing prebiotics have not been well studied with regard to the changes they trigger on the composition of the gut microbiota. This study evaluated the effect of a commercially available nutraceutical containing fructo-oligosaccharides (FOS) and inulin on the fecal microbiota of healthy cats and dogs when administered for 16 days. Fecal samples were collected at two time points before and at two time points during prebioticadministration. Totalgenomic DNA was obtained from fecal samples and 454 pyrosequencing was used for 16Sr RNA genebacterial profiling. The linear discriminant analysis (LDA) effectsize (LEfSe) method was used for detecting bacterial taxathatmay respond (i.e., increase or decrease in itsrelative abundance) to prebiotic administration. Prebiotic administration was associated with a good acceptance and no side effects (e.g., diarrhea) were reported by the owners. Alowdose of prebiotics (50 mL total regardless of body weight with the end product containing 0.45% of prebiotics) revealed a lower abundanceofGammaproteobacteriaandahigherabundanceofVeillonellaceaeduring prebiotic administration in cats, while Staphylococcaceae showed a higher abundance during prebiotic administration in dogs. These differences were not sufficient to separate bacterial communities as shown by analysis of weighted Uni Fracdistan cemetrics. A predictiveapproachofthefecalbacterialmetagenomeusingPhylogeneticInvestigation ofCommunitiesbyReconstructionofUnobservedStates(PICRUSt)alsodidnotreveal differences between the period before and during prebiotic administration. A second trial using a higher dose of prebiotics (3.2 mL/kg body weight with the end product containing 3.1% of prebiotics) was tested in dogs and revealed a lower abundance of Dorea (family Clostridiaceae) and a higher abundance of Megamonas and other (unknown) members of Veillonellaceae during prebiotic administration. Again, these changes were not sufficient to separate bacterial communities or predicted metabolic profiles according to treatment. A closer analysis of bacterial communities at all timepoints revealed highly individualized patterns of variation. This study shows a high interindividual variation of fecal bacterial communities from pet cats and dogs, that these communities are relatively stable over time, and that some of this variation can be attributable to prebiotic administration, a phenomenon that may be affected by the amount of the prebiotic administered in the formulation. This study also provides insights into the response of gut bacterial communities in pet cats and dogs during administrationofcommerciallyavailableproductscontainingprebiotics.Morestudies are needed to explore potentially beneficial effects on host health beyond changes in bacterial communities. Subjects Bioinformatics,Microbiology,VeterinaryMedicine,GastroenterologyandHepatology, Nutrition Keywords Microbiota,Prebiotics,Health,16SrRNAgene,Fructo-oligosaccharides,Inulin, Feline, Canine, Veterinary medicine, Gastrointestinal health 2017-04-18 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18262/1/551.pdf http://eprints.uanl.mx/18262/1.haspreviewThumbnailVersion/551.pdf García Mazcorro, José Francisco y Bárcenas Walls, José R. y Suchodolski, Jan S. y Steiner, Jörg M. (2017) Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing. PeerJ, 5. pp. 1-26. ISSN 2167-8359 http://doi.org/10.7717/peerj.3184 doi:10.7717/peerj.3184 |
| spellingShingle | SF Ganadería / Medicina veretrinaria García Mazcorro, José Francisco Bárcenas Walls, José R. Suchodolski, Jan S. Steiner, Jörg M. Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| title_full | Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| title_fullStr | Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| title_full_unstemmed | Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| title_short | Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo-oligosaccharides (FOS) and inulin using high-throughput 454-pyrosequencing |
| title_sort | molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo oligosaccharides fos and inulin using high throughput 454 pyrosequencing |
| topic | SF Ganadería / Medicina veretrinaria |
| url | http://eprints.uanl.mx/18262/1/551.pdf |
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