The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth
Abstract. Immunogenic cell death is a cell death modality that stimulates the immune system to combat cancer cells. IMMUNEPOTENT CRP (ICRP) is a mixture of substances of low molecular weight obtained from bovine spleens that exhibits in vitro cytotoxic activity on different tumor cell lines and modu...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/18250/1/542.pdf |
| _version_ | 1838551001483706368 |
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| author | Rodríguez Salazar, María del Carmen Franco Molina, Moisés Armides Mendoza Gamboa, Edgar Martínez Torres, Ana Carolina Zapata Benavides, Pablo López González, Jose Sullivan Coronado Cerda, Erika Evangelina Alcocer González, Juan Manuel Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_facet | Rodríguez Salazar, María del Carmen Franco Molina, Moisés Armides Mendoza Gamboa, Edgar Martínez Torres, Ana Carolina Zapata Benavides, Pablo López González, Jose Sullivan Coronado Cerda, Erika Evangelina Alcocer González, Juan Manuel Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_sort | Rodríguez Salazar, María del Carmen |
| collection | Repositorio Institucional |
| description | Abstract. Immunogenic cell death is a cell death modality that stimulates the immune system to combat cancer cells. IMMUNEPOTENT CRP (ICRP) is a mixture of substances of low molecular weight obtained from bovine spleens that exhibits in vitro cytotoxic activity on different tumor cell lines and modulates the immune response in vivo. The aim of the present study was to determine whether the cytotoxic effect of ICRP and its combination with oxaliplatin (OXP) on murine melanoma B16F10 cells was due to immunogenic cell death. The cytotoxic assay was performed using flow cytometry to detect Annexin V and propidium iodide staining, and calreticulin (CRT) exposure. Adenosine triphosphate, heat shock protein (HSP) 70, HSP90 and high mobility group box 1 (HMGB1) release were identified using bioluminescence, western blot and ELISA assays, respectively. The present in vitro study demonstrated that treatments with ICRP or OXP induced cell death in a time-dependent manner, but treatment with the combination of ICRP + OXP increased the cytotoxic effect following 24 h of treatment. CRT exposure and release of adenosine triphosphate (ATP), HSP70, HSP90 and HMGB1 were induced by treatment with ICRP, and the
combination of ICRP + OXP increased the exposure and release of damage-associated molecular patterns (DAMPs), while OXP treatment only induced CRT exposure, ATP and HMGB1 release. The in vivo experiments demonstrated that administration of tumor-derived DAMP-rich cell lysates derived from B16F10 cells treated with ICRP and the combination of ICRP + OXP prevented melanoma growth; however, OXP treatment did not. These results suggested that IMMUNEPOTENT CRP may be used as an agent to increase the ability of antitumor drugs to induce immunogenic cell death and prevent the growth of melanoma. |
| format | Article |
| id | eprints-18250 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-182502025-07-10T16:54:44Z http://eprints.uanl.mx/18250/ The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth Rodríguez Salazar, María del Carmen Franco Molina, Moisés Armides Mendoza Gamboa, Edgar Martínez Torres, Ana Carolina Zapata Benavides, Pablo López González, Jose Sullivan Coronado Cerda, Erika Evangelina Alcocer González, Juan Manuel Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina QH Historia Natural, Biología Abstract. Immunogenic cell death is a cell death modality that stimulates the immune system to combat cancer cells. IMMUNEPOTENT CRP (ICRP) is a mixture of substances of low molecular weight obtained from bovine spleens that exhibits in vitro cytotoxic activity on different tumor cell lines and modulates the immune response in vivo. The aim of the present study was to determine whether the cytotoxic effect of ICRP and its combination with oxaliplatin (OXP) on murine melanoma B16F10 cells was due to immunogenic cell death. The cytotoxic assay was performed using flow cytometry to detect Annexin V and propidium iodide staining, and calreticulin (CRT) exposure. Adenosine triphosphate, heat shock protein (HSP) 70, HSP90 and high mobility group box 1 (HMGB1) release were identified using bioluminescence, western blot and ELISA assays, respectively. The present in vitro study demonstrated that treatments with ICRP or OXP induced cell death in a time-dependent manner, but treatment with the combination of ICRP + OXP increased the cytotoxic effect following 24 h of treatment. CRT exposure and release of adenosine triphosphate (ATP), HSP70, HSP90 and HMGB1 were induced by treatment with ICRP, and the combination of ICRP + OXP increased the exposure and release of damage-associated molecular patterns (DAMPs), while OXP treatment only induced CRT exposure, ATP and HMGB1 release. The in vivo experiments demonstrated that administration of tumor-derived DAMP-rich cell lysates derived from B16F10 cells treated with ICRP and the combination of ICRP + OXP prevented melanoma growth; however, OXP treatment did not. These results suggested that IMMUNEPOTENT CRP may be used as an agent to increase the ability of antitumor drugs to induce immunogenic cell death and prevent the growth of melanoma. 2017-03-28 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18250/1/542.pdf http://eprints.uanl.mx/18250/1.haspreviewThumbnailVersion/542.pdf Rodríguez Salazar, María del Carmen y Franco Molina, Moisés Armides y Mendoza Gamboa, Edgar y Martínez Torres, Ana Carolina y Zapata Benavides, Pablo y López González, Jose Sullivan y Coronado Cerda, Erika Evangelina y Alcocer González, Juan Manuel y Tamez Guerra, Reyes Silvestre y Rodríguez Padilla, Cristina (2017) The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth. Oncology Letters, 14 (1). pp. 844-852. ISSN 1792-1074 http://doi.org/10.3892/ol.2017.6202 doi:10.3892/ol.2017.6202 |
| spellingShingle | QH Historia Natural, Biología Rodríguez Salazar, María del Carmen Franco Molina, Moisés Armides Mendoza Gamboa, Edgar Martínez Torres, Ana Carolina Zapata Benavides, Pablo López González, Jose Sullivan Coronado Cerda, Erika Evangelina Alcocer González, Juan Manuel Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| title_full | The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| title_fullStr | The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| title_full_unstemmed | The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| title_short | The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| title_sort | novel immunomodulator immunepotent crp combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth |
| topic | QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/18250/1/542.pdf |
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