Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation
Abstract. Melittin is the main component of bee venom consisting of 26 amino acids that has multiple effects, including antibacterial, antiviral and anti-inflammatory in various cell types. This peptide forms pores in biological membranes and triggers cell death. Therefore it has potential as an a...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/18214/1/505.pdf |
| _version_ | 1824415171333849088 |
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| author | González Horta, Azucena C. Matamoros Acosta, Arely Chávez Montes, Abelardo Castro Ríos, Rocío Lara Arias, Jorge |
| author_facet | González Horta, Azucena C. Matamoros Acosta, Arely Chávez Montes, Abelardo Castro Ríos, Rocío Lara Arias, Jorge |
| author_sort | González Horta, Azucena C. |
| collection | Repositorio Institucional |
| description | Abstract. Melittin is the main component of bee venom consisting of 26 amino acids that has multiple
effects, including antibacterial, antiviral and anti-inflammatory in various cell types. This peptide
forms pores in biological membranes and triggers cell death. Therefore it has potential as an anticancer therapy. However, the therapeutic application of melittin is limited due to its main side effect,
hemolysis, which is especially pronounced following intravenous administration. In the present study,
we formulated tetrameric melittin-carrying poly-D,L-lactic-co-glycolic acid nanoparticles (PLGANPs) and analyzed the lytic activity of this system on liposomes that resembles breast cancer cells.
Tetrameric melittin binds avidly to PLGA-NPs with an encapsulation efficiency of 97% and retains its
lytic activity demonstrating the effectiveness of PLGA-NPs as nanocarriers for this cytolytic peptide.
Key words: Melittin — PLGA nanoparticles — Liposomes — Leakage — Fluorescence — Circular
dichroism |
| format | Article |
| id | eprints-18214 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-182142024-03-06T15:11:52Z http://eprints.uanl.mx/18214/ Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation González Horta, Azucena C. Matamoros Acosta, Arely Chávez Montes, Abelardo Castro Ríos, Rocío Lara Arias, Jorge QH Historia Natural, Biología Abstract. Melittin is the main component of bee venom consisting of 26 amino acids that has multiple effects, including antibacterial, antiviral and anti-inflammatory in various cell types. This peptide forms pores in biological membranes and triggers cell death. Therefore it has potential as an anticancer therapy. However, the therapeutic application of melittin is limited due to its main side effect, hemolysis, which is especially pronounced following intravenous administration. In the present study, we formulated tetrameric melittin-carrying poly-D,L-lactic-co-glycolic acid nanoparticles (PLGANPs) and analyzed the lytic activity of this system on liposomes that resembles breast cancer cells. Tetrameric melittin binds avidly to PLGA-NPs with an encapsulation efficiency of 97% and retains its lytic activity demonstrating the effectiveness of PLGA-NPs as nanocarriers for this cytolytic peptide. Key words: Melittin — PLGA nanoparticles — Liposomes — Leakage — Fluorescence — Circular dichroism 2017-06-27 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18214/1/505.pdf http://eprints.uanl.mx/18214/1.haspreviewThumbnailVersion/505.pdf González Horta, Azucena C. y Matamoros Acosta, Arely y Chávez Montes, Abelardo y Castro Ríos, Rocío y Lara Arias, Jorge (2017) Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation. General physiology and biophysics, 36 (04). pp. 373-381. ISSN 1338-4325 http://doi.org/10.4149/gpb_2017011 doi:10.4149/gpb_2017011 |
| spellingShingle | QH Historia Natural, Biología González Horta, Azucena C. Matamoros Acosta, Arely Chávez Montes, Abelardo Castro Ríos, Rocío Lara Arias, Jorge Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| title_full | Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| title_fullStr | Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| title_full_unstemmed | Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| title_short | Biodegradable nanoparticles loaded with tetrameric melittin: preparation and membrane disruption evaluation |
| title_sort | biodegradable nanoparticles loaded with tetrameric melittin preparation and membrane disruption evaluation |
| topic | QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/18214/1/505.pdf |
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