Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage
This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var. israelensis after more than three decades of storage. For this purpose, a population of Aedes aegypti was established. The mortality rate of 20 spore-crystal extracts purifie...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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2017
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| Online Access: | http://eprints.uanl.mx/18205/1/495.pdf |
| _version_ | 1824415167942754304 |
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| author | Galán Wong, Luis Jesús Gamiño Hernández, Rossana Fernández Chapa, David Daniel García Díaz, Graciela De la Garza Ramos, Myriam Angélica Guajardo Barbosa, Claudio Luna Olvera, Hugo Alberto |
| author_facet | Galán Wong, Luis Jesús Gamiño Hernández, Rossana Fernández Chapa, David Daniel García Díaz, Graciela De la Garza Ramos, Myriam Angélica Guajardo Barbosa, Claudio Luna Olvera, Hugo Alberto |
| author_sort | Galán Wong, Luis Jesús |
| collection | Repositorio Institucional |
| description | This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var. israelensis
after more than three decades of storage. For this purpose, a population of Aedes aegypti was established. The mortality rate of
20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays
according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD50
and LD98. All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed
larvae at doses as low as 0.01 ppm. These data are surprising because no study on the activity of B. thuringiensis toxic proteins after
such a long storage time has been reported. |
| format | Article |
| id | eprints-18205 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-182052021-05-28T14:12:20Z http://eprints.uanl.mx/18205/ Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage Galán Wong, Luis Jesús Gamiño Hernández, Rossana Fernández Chapa, David Daniel García Díaz, Graciela De la Garza Ramos, Myriam Angélica Guajardo Barbosa, Claudio Luna Olvera, Hugo Alberto QR Microbiología This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var. israelensis after more than three decades of storage. For this purpose, a population of Aedes aegypti was established. The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD50 and LD98. All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed larvae at doses as low as 0.01 ppm. These data are surprising because no study on the activity of B. thuringiensis toxic proteins after such a long storage time has been reported. 2017-11-20 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18205/1/495.pdf http://eprints.uanl.mx/18205/1.haspreviewThumbnailVersion/495.pdf Galán Wong, Luis Jesús y Gamiño Hernández, Rossana y Fernández Chapa, David Daniel y García Díaz, Graciela y De la Garza Ramos, Myriam Angélica y Guajardo Barbosa, Claudio y Luna Olvera, Hugo Alberto (2017) Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage. International Journal of Microbiology, 2017. pp. 1-6. ISSN 1687-918X http://doi.org/10.1155/2017/5402748 doi:10.1155/2017/5402748 |
| spellingShingle | QR Microbiología Galán Wong, Luis Jesús Gamiño Hernández, Rossana Fernández Chapa, David Daniel García Díaz, Graciela De la Garza Ramos, Myriam Angélica Guajardo Barbosa, Claudio Luna Olvera, Hugo Alberto Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| title_full | Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| title_fullStr | Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| title_full_unstemmed | Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| title_short | Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage |
| title_sort | persistence of toxic activity of fermentation extracts from bacillus thuringiensis var israelensis after more than three decades of storage |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/18205/1/495.pdf |
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