Nutrition affects insect susceptibility to Bt toxins
Pesticide resistance represents a major challenge to global food production. The spread of resistance alleles is the primary explanation for observations of reduced pesticide efficacy over time, but the potential for gene-by-environment interactions (plasticity) to mediate susceptibility has large...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/18167/1/452.pdf |
| _version_ | 1824415153091772416 |
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| author | Deans, Carrie A. Behmer, Spencer T. Tessnow, Ashley E. Tamez Guerra, Patricia Pusztai Carey, Marianne Sword, Gregory A. |
| author_facet | Deans, Carrie A. Behmer, Spencer T. Tessnow, Ashley E. Tamez Guerra, Patricia Pusztai Carey, Marianne Sword, Gregory A. |
| author_sort | Deans, Carrie A. |
| collection | Repositorio Institucional |
| description | Pesticide resistance represents a major challenge to global food production. The spread of resistance
alleles is the primary explanation for observations of reduced pesticide efficacy over time, but the
potential for gene-by-environment interactions (plasticity) to mediate susceptibility has largely
been overlooked. Here we show that nutrition is an environmental factor that affects susceptibility
to Bt toxins. Protein and carbohydrates are two key macronutrients for insect herbivores, and the
polyphagous pest Helicoverpa zea self-selects and performs best on diets that are protein-biased
relative to carbohydrates. Despite this, most Bt bioassays employ carbohydrate-biased rearing
diets. This study explored the effect of diet protein-carbohydrate content on H. zea susceptibility to
Cry1Ac, a common Bt endotoxin. We detected a 100-fold increase in LC50 for larvae on optimal versus
carbohydrate-biased diets, and significant diet-mediated variation in survival and performance when
challenged with Cry1Ac. Our results suggest that Bt resistance bioassays that use ecologically- and
physiologically-mismatched diets over-estimate susceptibility and under-estimate resistance. |
| format | Article |
| id | eprints-18167 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-181672023-05-09T16:10:51Z http://eprints.uanl.mx/18167/ Nutrition affects insect susceptibility to Bt toxins Deans, Carrie A. Behmer, Spencer T. Tessnow, Ashley E. Tamez Guerra, Patricia Pusztai Carey, Marianne Sword, Gregory A. QH Historia Natural, Biología Pesticide resistance represents a major challenge to global food production. The spread of resistance alleles is the primary explanation for observations of reduced pesticide efficacy over time, but the potential for gene-by-environment interactions (plasticity) to mediate susceptibility has largely been overlooked. Here we show that nutrition is an environmental factor that affects susceptibility to Bt toxins. Protein and carbohydrates are two key macronutrients for insect herbivores, and the polyphagous pest Helicoverpa zea self-selects and performs best on diets that are protein-biased relative to carbohydrates. Despite this, most Bt bioassays employ carbohydrate-biased rearing diets. This study explored the effect of diet protein-carbohydrate content on H. zea susceptibility to Cry1Ac, a common Bt endotoxin. We detected a 100-fold increase in LC50 for larvae on optimal versus carbohydrate-biased diets, and significant diet-mediated variation in survival and performance when challenged with Cry1Ac. Our results suggest that Bt resistance bioassays that use ecologically- and physiologically-mismatched diets over-estimate susceptibility and under-estimate resistance. 2017-01-03 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18167/1/452.pdf http://eprints.uanl.mx/18167/1.haspreviewThumbnailVersion/452.pdf Deans, Carrie A. y Behmer, Spencer T. y Tessnow, Ashley E. y Tamez Guerra, Patricia y Pusztai Carey, Marianne y Sword, Gregory A. (2017) Nutrition affects insect susceptibility to Bt toxins. Scientific Reports, 7 (1). pp. 1-9. ISSN 2045-2322 http://doi.org/10.1038/srep39705 doi:10.1038/srep39705 |
| spellingShingle | QH Historia Natural, Biología Deans, Carrie A. Behmer, Spencer T. Tessnow, Ashley E. Tamez Guerra, Patricia Pusztai Carey, Marianne Sword, Gregory A. Nutrition affects insect susceptibility to Bt toxins |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Nutrition affects insect susceptibility to Bt toxins |
| title_full | Nutrition affects insect susceptibility to Bt toxins |
| title_fullStr | Nutrition affects insect susceptibility to Bt toxins |
| title_full_unstemmed | Nutrition affects insect susceptibility to Bt toxins |
| title_short | Nutrition affects insect susceptibility to Bt toxins |
| title_sort | nutrition affects insect susceptibility to bt toxins |
| topic | QH Historia Natural, Biología |
| url | http://eprints.uanl.mx/18167/1/452.pdf |
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