DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect
Abstract. Cellular immune responses play a critical role in the eradication of intracellular infections and malignant cells through the recognition and subsequent removal of the infection or malignant cells. Effective antigen presentation is crucial for stimulating the immune system against malignan...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/18129/1/426.pdf |
| _version_ | 1838551000080711680 |
|---|---|
| author | Pérez Trujillo, José Juan Garza Morales, Rodolfo Barrón Cantú, José A. Figueroa Parra, Gabriel García García, Aracely Rodríguez Rocha, Humberto García Juárez, Jaime Muñoz Maldonado, Gerardo Enrique Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto Loera Arias, María de Jesús |
| author_facet | Pérez Trujillo, José Juan Garza Morales, Rodolfo Barrón Cantú, José A. Figueroa Parra, Gabriel García García, Aracely Rodríguez Rocha, Humberto García Juárez, Jaime Muñoz Maldonado, Gerardo Enrique Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto Loera Arias, María de Jesús |
| author_sort | Pérez Trujillo, José Juan |
| collection | Repositorio Institucional |
| description | Abstract. Cellular immune responses play a critical role in the eradication of intracellular infections and malignant cells through the recognition and subsequent removal of the infection or malignant cells. Effective antigen presentation is crucial for stimulating the immune system against malignant cells. Calreticulin (CRT) has been used to improve antigen presentation. However, CRT overexpression has been previously associated with the development of pancreatic and breast cancer. The import and retention signals of CRT in the endoplasmic reticulum (ER) can be used to overcome CRT overexpression. The present study describes the potent antitumor effect of a DNA vaccine encoding human papillomavirus type 16 E6 and E7 antigens flanked by ER import and retention signals (SP-E6E7m-KDEL). The effect of this vaccine was compared with that of E6 and E7 antigens fused to human full-length CRT (hCRT-E6E7m). In the present
study, the effectiveness of SP-E6E7m-KDEL for inducing an interferon-γ antigen‑specific, response and its therapeutic effect against tumors was demonstrated, which was as effective as immunization against those antigens fused to CRT. This simplified strategy, using ER import and retention signal peptides to direct antigens to this organelle, provides an efficient alternative to traditional vaccines and, more importantly, a safe and potent system to induce a therapeutic antitumor response. |
| format | Article |
| id | eprints-18129 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-181292025-07-23T18:07:00Z http://eprints.uanl.mx/18129/ DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect Pérez Trujillo, José Juan Garza Morales, Rodolfo Barrón Cantú, José A. Figueroa Parra, Gabriel García García, Aracely Rodríguez Rocha, Humberto García Juárez, Jaime Muñoz Maldonado, Gerardo Enrique Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto Loera Arias, María de Jesús R Medicina en General Abstract. Cellular immune responses play a critical role in the eradication of intracellular infections and malignant cells through the recognition and subsequent removal of the infection or malignant cells. Effective antigen presentation is crucial for stimulating the immune system against malignant cells. Calreticulin (CRT) has been used to improve antigen presentation. However, CRT overexpression has been previously associated with the development of pancreatic and breast cancer. The import and retention signals of CRT in the endoplasmic reticulum (ER) can be used to overcome CRT overexpression. The present study describes the potent antitumor effect of a DNA vaccine encoding human papillomavirus type 16 E6 and E7 antigens flanked by ER import and retention signals (SP-E6E7m-KDEL). The effect of this vaccine was compared with that of E6 and E7 antigens fused to human full-length CRT (hCRT-E6E7m). In the present study, the effectiveness of SP-E6E7m-KDEL for inducing an interferon-γ antigen‑specific, response and its therapeutic effect against tumors was demonstrated, which was as effective as immunization against those antigens fused to CRT. This simplified strategy, using ER import and retention signal peptides to direct antigens to this organelle, provides an efficient alternative to traditional vaccines and, more importantly, a safe and potent system to induce a therapeutic antitumor response. 2017-10-13 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18129/1/426.pdf http://eprints.uanl.mx/18129/1.haspreviewThumbnailVersion/426.pdf Pérez Trujillo, José Juan y Garza Morales, Rodolfo y Barrón Cantú, José A. y Figueroa Parra, Gabriel y García García, Aracely y Rodríguez Rocha, Humberto y García Juárez, Jaime y Muñoz Maldonado, Gerardo Enrique y Saucedo Cárdenas, Odila y Montes de Oca Luna, Roberto y Loera Arias, María de Jesús (2017) DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect. Oncology Letters, 13 (3). pp. 1569-1574. ISSN 1792-1074 http://doi.org/10.3892/ol.2017.5635 doi:10.3892/ol.2017.5635 |
| spellingShingle | R Medicina en General Pérez Trujillo, José Juan Garza Morales, Rodolfo Barrón Cantú, José A. Figueroa Parra, Gabriel García García, Aracely Rodríguez Rocha, Humberto García Juárez, Jaime Muñoz Maldonado, Gerardo Enrique Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto Loera Arias, María de Jesús DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| title_full | DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| title_fullStr | DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| title_full_unstemmed | DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| title_short | DNA vaccine encoding human papillomavirus antigens flanked by a signal peptide and a KDEL sequence induces a potent therapeutic antitumor effect |
| title_sort | dna vaccine encoding human papillomavirus antigens flanked by a signal peptide and a kdel sequence induces a potent therapeutic antitumor effect |
| topic | R Medicina en General |
| url | http://eprints.uanl.mx/18129/1/426.pdf |
| work_keys_str_mv | AT pereztrujillojosejuan dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT garzamoralesrodolfo dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT barroncantujosea dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT figueroaparragabriel dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT garciagarciaaracely dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT rodriguezrochahumberto dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT garciajuarezjaime dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT munozmaldonadogerardoenrique dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT saucedocardenasodila dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT montesdeocalunaroberto dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect AT loeraariasmariadejesus dnavaccineencodinghumanpapillomavirusantigensflankedbyasignalpeptideandakdelsequenceinducesapotenttherapeuticantitumoreffect |