The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population
Abstract The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years±3....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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2017
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| Online Access: | http://eprints.uanl.mx/18047/1/397.pdf |
| _version_ | 1824415111110983680 |
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| author | Borrow, Ray González Andrade, Baltazar Santos Lartigue, Ramiro Flores Treviño, Samantha Maribel Ramirez Ochoa, Natalie Sonia Bocanegra Ibarias, Paola Huerta Torres, Francisco J. Mendoza Olazarán, Soraya Sarahí Villarreal Treviño, Licet Camacho Ortiz, Adrián Villarreal Vázquez, Hipólito Garza González, Elvira |
| author_facet | Borrow, Ray González Andrade, Baltazar Santos Lartigue, Ramiro Flores Treviño, Samantha Maribel Ramirez Ochoa, Natalie Sonia Bocanegra Ibarias, Paola Huerta Torres, Francisco J. Mendoza Olazarán, Soraya Sarahí Villarreal Treviño, Licet Camacho Ortiz, Adrián Villarreal Vázquez, Hipólito Garza González, Elvira |
| author_sort | Borrow, Ray |
| collection | Repositorio Institucional |
| description | Abstract
The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years±3.00; median age: 5 years, female: 58; age range: 1–15 years) and 195 nonrelated controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6%, 67/138) and Haemophilus influenzae (31.9%, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P<0.0001). Importantly, 41/54 (75.9%) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68%) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95% CI = 1.27– 14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population. |
| format | Article |
| id | eprints-18047 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-180472020-05-26T18:40:12Z http://eprints.uanl.mx/18047/ The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population Borrow, Ray González Andrade, Baltazar Santos Lartigue, Ramiro Flores Treviño, Samantha Maribel Ramirez Ochoa, Natalie Sonia Bocanegra Ibarias, Paola Huerta Torres, Francisco J. Mendoza Olazarán, Soraya Sarahí Villarreal Treviño, Licet Camacho Ortiz, Adrián Villarreal Vázquez, Hipólito Garza González, Elvira Abstract The aim of the present study was to estimate the relative contribution of immunogenetic and microbiological factors in the development of recurrent tonsillitis in a Mexican population. Patients (n = 138) with recurrent tonsillitis and an indication of tonsillectomy (mean age: 6.05 years±3.00; median age: 5 years, female: 58; age range: 1–15 years) and 195 nonrelated controls older than 18 years and a medical history free of recurrent tonsillitis were included. To evaluate the microbial contribution, tonsil swab samples from both groups and extracted tonsil samples from cases were cultured. Biofilm production of isolated bacteria was measured. To assess the immunogenetic component, DNA from peripheral blood was genotyped for the TNFA-308G/A single-nucleotide polymorphism (SNP) and for the IL1B -31C/T SNP. Normal microbiota, but no pathogens or potential pathogens, were identified from all control sample cultures. The most frequent pathogenic species detected in tonsils from cases were Staphylococcus aureus (48.6%, 67/138) and Haemophilus influenzae (31.9%, 44/138), which were found more frequently in patient samples than in samples from healthy volunteers (P<0.0001). Importantly, 41/54 (75.9%) S. aureus isolates were biofilm producers (18 weak and 23 strong), whereas 17/25 (68%) H. influenzae isolates were biofilm producers (10 weak, and 7 strong biofilm producers). Patients with at least one copy of the IL1B-31*C allele had a higher risk of recurrent tonsillitis (OR = 4.03; 95% CI = 1.27– 14.27; P = 0.013). TNFA-308 G/A alleles were not preferentially distributed among the groups. When considering the presence of IL1B-31*C plus S. aureus, IL1B-31*C plus S. aureus biofilm producer, IL1B-31*C plus H. influenzae or IL1B-31*C plus H. influenzae biofilm producer, the OR tended to infinite. Thus, the presence of IL1B-31*C allele plus the presence of S. aureus and/or H. influenzae could be related to the development of tonsillitis in this particular Mexican population. 2017-05-24 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/18047/1/397.pdf http://eprints.uanl.mx/18047/1.haspreviewThumbnailVersion/397.pdf Borrow, Ray y González Andrade, Baltazar y Santos Lartigue, Ramiro y Flores Treviño, Samantha Maribel y Ramirez Ochoa, Natalie Sonia y Bocanegra Ibarias, Paola y Huerta Torres, Francisco J. y Mendoza Olazarán, Soraya Sarahí y Villarreal Treviño, Licet y Camacho Ortiz, Adrián y Villarreal Vázquez, Hipólito y Garza González, Elvira (2017) The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population. PLOS ONE, 12 (5). pp. 1-8. ISSN 1932-6203 http://doi.org/10.1371/journal.pone.0178115 doi:10.1371/journal.pone.0178115 |
| spellingShingle | Borrow, Ray González Andrade, Baltazar Santos Lartigue, Ramiro Flores Treviño, Samantha Maribel Ramirez Ochoa, Natalie Sonia Bocanegra Ibarias, Paola Huerta Torres, Francisco J. Mendoza Olazarán, Soraya Sarahí Villarreal Treviño, Licet Camacho Ortiz, Adrián Villarreal Vázquez, Hipólito Garza González, Elvira The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| title_full | The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| title_fullStr | The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| title_full_unstemmed | The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| title_short | The carriage of interleukin-1B-31*C allele plus Staphylococcus aureus and Haemophilus influenzae increases the risk of recurrent tonsillitis in a Mexican population |
| title_sort | carriage of interleukin 1b 31 c allele plus staphylococcus aureus and haemophilus influenzae increases the risk of recurrent tonsillitis in a mexican population |
| url | http://eprints.uanl.mx/18047/1/397.pdf |
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