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Expression of the HPV18/E6 oncoprotein induces DNA damage

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Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human...

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Bibliographic Details
Main Authors: Cortés Gutiérrez, Elva Irene, García Vielma, Catalina, Aguilar Lemarroy, Adriana, Vallejo Ruíz, Veronica, Piña Sánchez, Patricia, Zapata Benavides, Pablo, Gosálvez, Jaime
Format: Article
Language:English
Published: 2017
Online Access:http://eprints.uanl.mx/17590/1/376.pdf
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Summary:Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene.

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