Expression of the HPV18/E6 oncoprotein induces DNA damage
Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2017
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| Online Access: | http://eprints.uanl.mx/17590/1/376.pdf |
| _version_ | 1824414945867988992 |
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| author | Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime |
| author_facet | Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime |
| author_sort | Cortés Gutiérrez, Elva Irene |
| collection | Repositorio Institucional |
| description | Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. |
| format | Article |
| id | eprints-17590 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-175902021-04-19T15:00:19Z http://eprints.uanl.mx/17590/ Expression of the HPV18/E6 oncoprotein induces DNA damage Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. 2017 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/17590/1/376.pdf http://eprints.uanl.mx/17590/1.haspreviewThumbnailVersion/376.pdf Cortés Gutiérrez, Elva Irene y García Vielma, Catalina y Aguilar Lemarroy, Adriana y Vallejo Ruíz, Veronica y Piña Sánchez, Patricia y Zapata Benavides, Pablo y Gosálvez, Jaime (2017) Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61 (2). pp. 96-101. ISSN 1121-760X http://doi.org/10.4081/ejh.2017.2773 doi:10.4081/ejh.2017.2773 |
| spellingShingle | Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime Expression of the HPV18/E6 oncoprotein induces DNA damage |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_fullStr | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full_unstemmed | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_short | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_sort | expression of the hpv18 e6 oncoprotein induces dna damage |
| url | http://eprints.uanl.mx/17590/1/376.pdf |
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