Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating
Considerable effort has been focused on improving the control of size, shape, and surface modifications to detect proteins. The purposeofthisstudywastocomparetheefficienciesofaminosilane-coatedmagnetite(As-M)nanoparticles(NPs),dextran-coated magnetitenanoparticles(Dx-M),andbarenanoparticlesforconjug...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/17415/1/275.pdf |
| _version_ | 1824414891002298368 |
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| author | Villegas Serralta, Enrique Zavala, Oscar Flores Urquizo, Israel Alejandro García Casillas, Perla E. Chapa González, Christian |
| author_facet | Villegas Serralta, Enrique Zavala, Oscar Flores Urquizo, Israel Alejandro García Casillas, Perla E. Chapa González, Christian |
| author_sort | Villegas Serralta, Enrique |
| collection | Repositorio Institucional |
| description | Considerable effort has been focused on improving the control of size, shape, and surface modifications to detect proteins. The purposeofthisstudywastocomparetheefficienciesofaminosilane-coatedmagnetite(As-M)nanoparticles(NPs),dextran-coated magnetitenanoparticles(Dx-M),andbarenanoparticlesforconjugatingsingle-chainvariablefragmentantibodies(scFvs)withthe aim of detecting the human epidermal growth factor receptor 2 (HER2) protein. Dx-M and As-M NPs were characterized using scanningelectronmicroscopy,energydispersiveX-rayspectroscopy,X-raydiffraction,andRamanspectroscopy.Dx-MandAs-M wereconjugatedwithamonoclonalscFvforactivetargetingoftheHER2antigen.AminosilanesurfacecoatingenhancedthescFv conjugationefficiencyovertwofoldcomparedtothatofthedextran-coatedmagnetiteNPsforthedetectionofHER2proteins. |
| format | Article |
| id | eprints-17415 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-174152020-05-01T16:43:15Z http://eprints.uanl.mx/17415/ Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating Villegas Serralta, Enrique Zavala, Oscar Flores Urquizo, Israel Alejandro García Casillas, Perla E. Chapa González, Christian Considerable effort has been focused on improving the control of size, shape, and surface modifications to detect proteins. The purposeofthisstudywastocomparetheefficienciesofaminosilane-coatedmagnetite(As-M)nanoparticles(NPs),dextran-coated magnetitenanoparticles(Dx-M),andbarenanoparticlesforconjugatingsingle-chainvariablefragmentantibodies(scFvs)withthe aim of detecting the human epidermal growth factor receptor 2 (HER2) protein. Dx-M and As-M NPs were characterized using scanningelectronmicroscopy,energydispersiveX-rayspectroscopy,X-raydiffraction,andRamanspectroscopy.Dx-MandAs-M wereconjugatedwithamonoclonalscFvforactivetargetingoftheHER2antigen.AminosilanesurfacecoatingenhancedthescFv conjugationefficiencyovertwofoldcomparedtothatofthedextran-coatedmagnetiteNPsforthedetectionofHER2proteins. 2018-04-02 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/17415/1/275.pdf http://eprints.uanl.mx/17415/1.haspreviewThumbnailVersion/275.pdf Villegas Serralta, Enrique y Zavala, Oscar y Flores Urquizo, Israel Alejandro y García Casillas, Perla E. y Chapa González, Christian (2018) Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating. Journal of Nanomaterials, 2018. pp. 1-9. ISSN 1687-4110 http://doi.org/10.1155/2018/7571613 doi:10.1155/2018/7571613 |
| spellingShingle | Villegas Serralta, Enrique Zavala, Oscar Flores Urquizo, Israel Alejandro García Casillas, Perla E. Chapa González, Christian Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| title_full | Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| title_fullStr | Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| title_full_unstemmed | Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| title_short | Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating |
| title_sort | detection of her2 through antibody immobilization is influenced by the properties of the magnetite nanoparticle coating |
| url | http://eprints.uanl.mx/17415/1/275.pdf |
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