In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells
Aim: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. Materials and methods: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mamma...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Dove Medical Press
2018
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/17156/1/260.pdf |
| _version_ | 1824414809217564672 |
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| author | Hernández Delgadillo, René García Cuellar, Claudia María Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Martínez Martínez, Marco Antonio Rangel Padilla, Eyra Elvyra Nakagoshi Cepeda, Sergio Eduardo Solís Soto, Juan Manuel Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_facet | Hernández Delgadillo, René García Cuellar, Claudia María Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Martínez Martínez, Marco Antonio Rangel Padilla, Eyra Elvyra Nakagoshi Cepeda, Sergio Eduardo Solís Soto, Juan Manuel Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio |
| author_sort | Hernández Delgadillo, René |
| collection | Repositorio Institucional |
| description | Aim: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. Materials and methods: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mammary epitheliocytes as noncancer control cells. Cell viability was evaluated with the MTT assay, plasma membrane integrity was analyzed with the calcein AM assay, genotoxicity with the comet assay, and apoptosis with the Annexin V/7-AAD assay. Results: BisBAL NPs were spherical in shape (average diameter, 28 nm) and agglomerated into dense electronic clusters. BisBAL NP induced a dose-dependent growth inhibition. Most importantly, growth inhibition was higher for MCF-7 cells than for MCF-10A cells. At 1 µM BisBAL NP, MCF-7 growth inhibition was 51%, while it was 11% for MCF-10A; at 25 µM BisBAL NP, the growth inhibition was 81% for MCF-7 and 24% for MCF-10A. With respect to mechanisms of action, a 24-hour exposure of 10 and 100 µM BisBAL NP caused loss of cell membrane integrity and fragmentation of tumor cell DNA. BisBAL NPs at 10 µM were genotoxic to and caused apoptosis of breast cancer cells. Conclusion: BisBAL NP-induced growth inhibition is dose dependent, and breast cancer cells are more vulnerable than noncancer breast cells. The mechanism of action of BisBAL NPs may include loss of plasma membrane integrity and a genotoxic effect on the genomic DNA of breast cancer cells. Keywords: antitumor activity, bismuth nanoparticles, breast cancer, chemotherapy, cytotoxicity |
| format | Article |
| id | eprints-17156 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| publisher | Dove Medical Press |
| record_format | eprints |
| spelling | eprints-171562020-05-18T20:12:10Z http://eprints.uanl.mx/17156/ In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells Hernández Delgadillo, René García Cuellar, Claudia María Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Martínez Martínez, Marco Antonio Rangel Padilla, Eyra Elvyra Nakagoshi Cepeda, Sergio Eduardo Solís Soto, Juan Manuel Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio R Medicina en General Aim: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. Materials and methods: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mammary epitheliocytes as noncancer control cells. Cell viability was evaluated with the MTT assay, plasma membrane integrity was analyzed with the calcein AM assay, genotoxicity with the comet assay, and apoptosis with the Annexin V/7-AAD assay. Results: BisBAL NPs were spherical in shape (average diameter, 28 nm) and agglomerated into dense electronic clusters. BisBAL NP induced a dose-dependent growth inhibition. Most importantly, growth inhibition was higher for MCF-7 cells than for MCF-10A cells. At 1 µM BisBAL NP, MCF-7 growth inhibition was 51%, while it was 11% for MCF-10A; at 25 µM BisBAL NP, the growth inhibition was 81% for MCF-7 and 24% for MCF-10A. With respect to mechanisms of action, a 24-hour exposure of 10 and 100 µM BisBAL NP caused loss of cell membrane integrity and fragmentation of tumor cell DNA. BisBAL NPs at 10 µM were genotoxic to and caused apoptosis of breast cancer cells. Conclusion: BisBAL NP-induced growth inhibition is dose dependent, and breast cancer cells are more vulnerable than noncancer breast cells. The mechanism of action of BisBAL NPs may include loss of plasma membrane integrity and a genotoxic effect on the genomic DNA of breast cancer cells. Keywords: antitumor activity, bismuth nanoparticles, breast cancer, chemotherapy, cytotoxicity Dove Medical Press 2018 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/17156/1/260.pdf http://eprints.uanl.mx/17156/1.haspreviewThumbnailVersion/260.pdf Hernández Delgadillo, René y García Cuellar, Claudia María y Sánchez Pérez, Yesennia y Pineda Aguilar, Nayely y Martínez Martínez, Marco Antonio y Rangel Padilla, Eyra Elvyra y Nakagoshi Cepeda, Sergio Eduardo y Solís Soto, Juan Manuel y Sánchez Nájera, Rosa Isela y Nakagoshi Cepeda, María Argelia Akemi y Chellam, Shankararaman y Cabral Romero, Claudio (2018) In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells. International Journal of Nanomedicine, 13. pp. 6089-6097. ISSN 1178-2013 http://doi.org/10.2147/IJN.S179095 doi:10.2147/IJN.S179095 |
| spellingShingle | R Medicina en General Hernández Delgadillo, René García Cuellar, Claudia María Sánchez Pérez, Yesennia Pineda Aguilar, Nayely Martínez Martínez, Marco Antonio Rangel Padilla, Eyra Elvyra Nakagoshi Cepeda, Sergio Eduardo Solís Soto, Juan Manuel Sánchez Nájera, Rosa Isela Nakagoshi Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral Romero, Claudio In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| title_full | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| title_fullStr | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| title_full_unstemmed | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| title_short | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
| title_sort | in vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles bisbal nps on breast cancer cells |
| topic | R Medicina en General |
| url | http://eprints.uanl.mx/17156/1/260.pdf |
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