In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS
Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused by Entamoeba histolytica (E. histolytica), is considered endemic in Mexico, where Argemone mexicana (A. mexicana) has been used in traditional medicine to treat intesti...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Hindawi Publishing Corporation
2018
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| Acceso en línea: | http://eprints.uanl.mx/17152/1/255.pdf |
| _version_ | 1824414807636312064 |
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| author | Elizondo Luévano, Joel Horacio Castro Ríos, Rocío Sánchez García, Eduardo Hernández García, Magda Elizabeth Vargas Villarreal, Javier Rodríguez Luis, Osvelia Esmeralda Chávez Montes, Abelardo |
| author_facet | Elizondo Luévano, Joel Horacio Castro Ríos, Rocío Sánchez García, Eduardo Hernández García, Magda Elizabeth Vargas Villarreal, Javier Rodríguez Luis, Osvelia Esmeralda Chávez Montes, Abelardo |
| author_sort | Elizondo Luévano, Joel Horacio |
| collection | Repositorio Institucional |
| description | Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused by Entamoeba histolytica (E. histolytica), is considered endemic in Mexico, where Argemone mexicana (A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. ,e objective of this work was to evaluate the potential biological activity of A. mexicana on E. histolytica. For this purpose, a methanolic extract was prepared from A. mexicana leaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain of Entamoeba histolytica. A. mexicana extract was found to have a growth-inhibiting activity for E. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The
identification of amoebicidal activity of A. mexicana on E. histolytica gives su pport to the traditional use. Further studies withberberine and jatrorrhizine will be carried out to understand the mechanism involved. |
| format | Article |
| id | eprints-17152 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| publisher | Hindawi Publishing Corporation |
| record_format | eprints |
| spelling | eprints-171522024-12-11T15:29:16Z http://eprints.uanl.mx/17152/ In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS Elizondo Luévano, Joel Horacio Castro Ríos, Rocío Sánchez García, Eduardo Hernández García, Magda Elizabeth Vargas Villarreal, Javier Rodríguez Luis, Osvelia Esmeralda Chávez Montes, Abelardo QR Microbiología RC Medicina Interna, Psiquiatría, Neurología Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused by Entamoeba histolytica (E. histolytica), is considered endemic in Mexico, where Argemone mexicana (A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. ,e objective of this work was to evaluate the potential biological activity of A. mexicana on E. histolytica. For this purpose, a methanolic extract was prepared from A. mexicana leaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain of Entamoeba histolytica. A. mexicana extract was found to have a growth-inhibiting activity for E. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The identification of amoebicidal activity of A. mexicana on E. histolytica gives su pport to the traditional use. Further studies withberberine and jatrorrhizine will be carried out to understand the mechanism involved. Hindawi Publishing Corporation 2018-07-30 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/17152/1/255.pdf http://eprints.uanl.mx/17152/1.haspreviewThumbnailVersion/255.pdf Elizondo Luévano, Joel Horacio y Castro Ríos, Rocío y Sánchez García, Eduardo y Hernández García, Magda Elizabeth y Vargas Villarreal, Javier y Rodríguez Luis, Osvelia Esmeralda y Chávez Montes, Abelardo (2018) In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS. Canadian journal of infectious diseases and medical microbiology, 2018. pp. 1-8. ISSN 1712-9532 http://doi.org/10.1155/2018/7453787 doi:10.1155/2018/7453787 |
| spellingShingle | QR Microbiología RC Medicina Interna, Psiquiatría, Neurología Elizondo Luévano, Joel Horacio Castro Ríos, Rocío Sánchez García, Eduardo Hernández García, Magda Elizabeth Vargas Villarreal, Javier Rodríguez Luis, Osvelia Esmeralda Chávez Montes, Abelardo In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| title_full | In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| title_fullStr | In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| title_full_unstemmed | In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| title_short | In Vitro Study of Antiamoebic Activity of Methanol Extracts of Argemone mexicana on Trophozoites of Entamoeba histolytica HM1-IMSS |
| title_sort | in vitro study of antiamoebic activity of methanol extracts of argemone mexicana on trophozoites of entamoeba histolytica hm1 imss |
| topic | QR Microbiología RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/17152/1/255.pdf |
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