UPLC-ESI-QTOF-MS2-Based Identification and Antioxidant Activity Assessment of Phenolic Compounds from Red Corn Cob (Zea mays L.)

Abstract: In this study, the extraction of phenolic antioxidants from red corn cob was carried out usingultrasound-assistedextraction(UAE).Thesolid:liquidratioandextractiontimewereevaluated when obtaining these bioactive compounds. The total phenolic contents were evaluated using the Folin Ciocalteu...

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Bibliographic Details
Main Authors: Hernández, María, Ventura, Janeth, Castro, Cecilia, Boone, Víctor, Rojas, Romeo, Ascacio Valdés, Juan, Martínez Ávila, Guillermo Cristian Guadalupe
Format: Article
Language:English
Published: 2018
Online Access:http://eprints.uanl.mx/16635/1/231.pdf
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Summary:Abstract: In this study, the extraction of phenolic antioxidants from red corn cob was carried out usingultrasound-assistedextraction(UAE).Thesolid:liquidratioandextractiontimewereevaluated when obtaining these bioactive compounds. The total phenolic contents were evaluated using the Folin Ciocalteu method, while the antioxidant activity was measured by ABTS•+ and DPPH• assays. The amount of phenolic compounds ranged from 215.17±33.49 to 527.33±103.79 GAE mg/100 g and, overall, high solid:liquid ratios and time periods release more phenolic compounds. Moreover, theredcorncobextractsshowedhigherradicalscavengingcapacityaccordingtotheresultsobtained usingtheABTS•+ techniquecomparedtotheDPPH• test. Thecouplingofliquidchromatographyand mass spectrometry assay allowed the determination of 11 phenolic compounds, including phenolic acidsandflavonoids. Thus,ourresultsdemonstratedforthefirsttimethepotentialofredcorncobas asourceofbioactivecompounds,whichmightbeincludedinfoodandpharmacologicalpreparations. Keywords: phenols compounds; red corn cob; ultrasonic-assisted extraction; UPLC-MS2