CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients
Abstract. Vitiligo is a disorder causing skin depigmentation, in which several factors have been proposed for its pathogenesis: Environmental, genetic and biological aspects of melanocytes, even those of the surrounding keratinocytes. However, the lack of understanding of the mechanisms has complica...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/16321/1/166.pdf |
| _version_ | 1824414575904161792 |
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| author | Salinas Santander, Mauricio Andrés Treviño Alvarado, Víctor Manuel De la Rosa Moreno, Eduardo I. Verduzco Garza, Bárbara Sánchez Domínguez, Celia Nohemí Cantú Salinas, Cristina Susana Ocampo Garza, Jorge Lagos Rodríguez, Armando Ocampo Candiani, Jorge Ortiz López, Rocío |
| author_facet | Salinas Santander, Mauricio Andrés Treviño Alvarado, Víctor Manuel De la Rosa Moreno, Eduardo I. Verduzco Garza, Bárbara Sánchez Domínguez, Celia Nohemí Cantú Salinas, Cristina Susana Ocampo Garza, Jorge Lagos Rodríguez, Armando Ocampo Candiani, Jorge Ortiz López, Rocío |
| author_sort | Salinas Santander, Mauricio Andrés |
| collection | Repositorio Institucional |
| description | Abstract. Vitiligo is a disorder causing skin depigmentation, in which several factors have been proposed for its pathogenesis: Environmental, genetic and biological aspects of melanocytes, even those of the surrounding keratinocytes. However, the lack of understanding of the mechanisms has complicated the task of predicting the development and progression. The present study used microarray analysis to characterize the transcriptional profile of skin from Vitiligo Vulgaris (VV) patients and the identified transcripts were validated using targeted high-throughput RNA sequencing in a broader set of patients. For microarrays, mRNA was taken from 20 skin biopsies of 10 patients with VV (pigmented and depigmented skin biopsy of each), and 5 biopsies of healthy subjects matched for age and sex were used as a control. A signature was identified that contains the expression pattern of 722 genes between depigmented vitiligo skin vs. healthy control, 1,108 between the pigmented skin of vitiligo vs. healthy controls and 1,927 between pigmented skin, depigmented vitiligo and healthy controls (P<0.05; false discovery rate, <0.1). When comparing the pigmented and depigmented skin of patients with vitiligo, which reflects the real difference between both skin types, 5 differentially expressed genes were identified and further validated in 45 additional VV patients
by RNA sequencing. This analysis showed significantly higher RNA levels of calpain-3, dopachrome tautomerase, melan-A and tyrosinase-related protein-1 genes. The data revealed that the pigmented skin of vitiligo is already affected at the level of gene expression and that the main differences between pigmented and non-pigmented skin are explained by the expression of genes associated with pigment metabolism. |
| format | Article |
| id | eprints-16321 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-163212020-05-06T16:12:15Z http://eprints.uanl.mx/16321/ CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients Salinas Santander, Mauricio Andrés Treviño Alvarado, Víctor Manuel De la Rosa Moreno, Eduardo I. Verduzco Garza, Bárbara Sánchez Domínguez, Celia Nohemí Cantú Salinas, Cristina Susana Ocampo Garza, Jorge Lagos Rodríguez, Armando Ocampo Candiani, Jorge Ortiz López, Rocío Abstract. Vitiligo is a disorder causing skin depigmentation, in which several factors have been proposed for its pathogenesis: Environmental, genetic and biological aspects of melanocytes, even those of the surrounding keratinocytes. However, the lack of understanding of the mechanisms has complicated the task of predicting the development and progression. The present study used microarray analysis to characterize the transcriptional profile of skin from Vitiligo Vulgaris (VV) patients and the identified transcripts were validated using targeted high-throughput RNA sequencing in a broader set of patients. For microarrays, mRNA was taken from 20 skin biopsies of 10 patients with VV (pigmented and depigmented skin biopsy of each), and 5 biopsies of healthy subjects matched for age and sex were used as a control. A signature was identified that contains the expression pattern of 722 genes between depigmented vitiligo skin vs. healthy control, 1,108 between the pigmented skin of vitiligo vs. healthy controls and 1,927 between pigmented skin, depigmented vitiligo and healthy controls (P<0.05; false discovery rate, <0.1). When comparing the pigmented and depigmented skin of patients with vitiligo, which reflects the real difference between both skin types, 5 differentially expressed genes were identified and further validated in 45 additional VV patients by RNA sequencing. This analysis showed significantly higher RNA levels of calpain-3, dopachrome tautomerase, melan-A and tyrosinase-related protein-1 genes. The data revealed that the pigmented skin of vitiligo is already affected at the level of gene expression and that the main differences between pigmented and non-pigmented skin are explained by the expression of genes associated with pigment metabolism. 2018-01-05 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16321/1/166.pdf http://eprints.uanl.mx/16321/1.haspreviewThumbnailVersion/166.pdf Salinas Santander, Mauricio Andrés y Treviño Alvarado, Víctor Manuel y De la Rosa Moreno, Eduardo I. y Verduzco Garza, Bárbara y Sánchez Domínguez, Celia Nohemí y Cantú Salinas, Cristina Susana y Ocampo Garza, Jorge y Lagos Rodríguez, Armando y Ocampo Candiani, Jorge y Ortiz López, Rocío (2018) CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients. Experimental and Therapeutic Medicine. pp. 2804-2811. ISSN 1792-0981 http://doi.org/10.3892/etm.2018.5764 doi:10.3892/etm.2018.5764 |
| spellingShingle | Salinas Santander, Mauricio Andrés Treviño Alvarado, Víctor Manuel De la Rosa Moreno, Eduardo I. Verduzco Garza, Bárbara Sánchez Domínguez, Celia Nohemí Cantú Salinas, Cristina Susana Ocampo Garza, Jorge Lagos Rodríguez, Armando Ocampo Candiani, Jorge Ortiz López, Rocío CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| title_full | CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| title_fullStr | CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| title_full_unstemmed | CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| title_short | CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients |
| title_sort | capn3 dct mlana and tyrp1 are overexpressed in skin of vitiligo vulgaris mexican patients |
| url | http://eprints.uanl.mx/16321/1/166.pdf |
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