Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes
BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instabili...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2018
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| Acceso en línea: | http://eprints.uanl.mx/16319/1/164.pdf |
| _version_ | 1824414575095709696 |
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| author | García Pérez, Oscar Daniel Cruz, Julio Ramirez, Carlos Villarreal, David Gamboa Delgado, Julián |
| author_facet | García Pérez, Oscar Daniel Cruz, Julio Ramirez, Carlos Villarreal, David Gamboa Delgado, Julián |
| author_sort | García Pérez, Oscar Daniel |
| collection | Repositorio Institucional |
| description | BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection. |
| format | Article |
| id | eprints-16319 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-163192021-06-02T15:39:31Z http://eprints.uanl.mx/16319/ Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes García Pérez, Oscar Daniel Cruz, Julio Ramirez, Carlos Villarreal, David Gamboa Delgado, Julián BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection. 2018-03 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16319/1/164.pdf http://eprints.uanl.mx/16319/1.haspreviewThumbnailVersion/164.pdf García Pérez, Oscar Daniel y Cruz, Julio y Ramirez, Carlos y Villarreal, David y Gamboa Delgado, Julián (2018) Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes. Latin American Journal of Aquatic Research, 46 (1). pp. 37-44. ISSN 0718560X http://doi.org/10.3856/vol46-issue1-fulltext-5 doi:10.3856/vol46-issue1-fulltext-5 |
| spellingShingle | García Pérez, Oscar Daniel Cruz, Julio Ramirez, Carlos Villarreal, David Gamboa Delgado, Julián Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| title_full | Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| title_fullStr | Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| title_full_unstemmed | Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| title_short | Exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish Ictalurus punctatus by means of nitrogen stable isotopes |
| title_sort | exploring the contribution of dietary protein from poultry by product meal and fish meal to the growth of catfish ictalurus punctatus by means of nitrogen stable isotopes |
| url | http://eprints.uanl.mx/16319/1/164.pdf |
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