Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment
ABSTRACT IMMUNEPOTENT CRP is a dialyzable leukocyte extract obtained from bovine spleen with immunomodulatory and antitumour properties; therefore, when administrated as an adjuvant therapy for cancer patients, it has increased their survival and quality of life. The bioavailability of any orally ad...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2018
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| Acceso en línea: | http://eprints.uanl.mx/16182/1/97.pdf |
| _version_ | 1838550996702199808 |
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| author | Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Coronado Cerda, Erika Evangelina Hernández Luna, Carlos Eduardo Zárate Triviño, Diana Ginette Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Salazar, María del Carmen Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_facet | Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Coronado Cerda, Erika Evangelina Hernández Luna, Carlos Eduardo Zárate Triviño, Diana Ginette Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Salazar, María del Carmen Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina |
| author_sort | Franco Molina, Moisés Armides |
| collection | Repositorio Institucional |
| description | ABSTRACT IMMUNEPOTENT CRP is a dialyzable leukocyte extract obtained from bovine spleen with immunomodulatory and antitumour properties; therefore, when administrated as an adjuvant therapy for cancer patients, it has increased their survival and quality of life. The bioavailability of any orally administered compound can be reduced due to gastrointestinal enzymes. In this study, we evaluated if IMMUNEPOTENT CRP is resistant to the treatment with different enzymes (proteases, nucleases, polysaccharide-degrading enzymes or lipase), using as parameters for biological activity measurement its in vitro antitumour and antioxidant properties and in vivo the antitumour effect of IMMUNEPOTENT CRP treated with proteinase K. In conclusion, we consider necessary to include the antioxidant and cytotoxic activity on the MCF-7 cancer cell line as parameters for the quantitative determination of biological activity or potency tests for batch release. Additionally, the results showed that different enzymatic treatments do not affect the antitumour and antioxidant activities of IMMUNEPOTENT CRP in vitro, suggesting that this product can be administered orally without any loss of biological activity. Furthermore, IMMUNEPOTENT CRP treatment with proteinase K increases the antitumour activity in vivo. |
| format | Article |
| id | eprints-16182 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-161822025-07-10T16:54:02Z http://eprints.uanl.mx/16182/ Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Coronado Cerda, Erika Evangelina Hernández Luna, Carlos Eduardo Zárate Triviño, Diana Ginette Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Salazar, María del Carmen Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina ABSTRACT IMMUNEPOTENT CRP is a dialyzable leukocyte extract obtained from bovine spleen with immunomodulatory and antitumour properties; therefore, when administrated as an adjuvant therapy for cancer patients, it has increased their survival and quality of life. The bioavailability of any orally administered compound can be reduced due to gastrointestinal enzymes. In this study, we evaluated if IMMUNEPOTENT CRP is resistant to the treatment with different enzymes (proteases, nucleases, polysaccharide-degrading enzymes or lipase), using as parameters for biological activity measurement its in vitro antitumour and antioxidant properties and in vivo the antitumour effect of IMMUNEPOTENT CRP treated with proteinase K. In conclusion, we consider necessary to include the antioxidant and cytotoxic activity on the MCF-7 cancer cell line as parameters for the quantitative determination of biological activity or potency tests for batch release. Additionally, the results showed that different enzymatic treatments do not affect the antitumour and antioxidant activities of IMMUNEPOTENT CRP in vitro, suggesting that this product can be administered orally without any loss of biological activity. Furthermore, IMMUNEPOTENT CRP treatment with proteinase K increases the antitumour activity in vivo. 2018-03-30 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16182/1/97.pdf http://eprints.uanl.mx/16182/1.haspreviewThumbnailVersion/97.pdf Franco Molina, Moisés Armides y Santana Krimskaya, Silvia Elena y Coronado Cerda, Erika Evangelina y Hernández Luna, Carlos Eduardo y Zárate Triviño, Diana Ginette y Zapata Benavides, Pablo y Mendoza Gamboa, Edgar y Rodríguez Salazar, María del Carmen y Tamez Guerra, Reyes Silvestre y Rodríguez Padilla, Cristina (2018) Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment. Biotechnology & biotechnological equipment, 32 (4). pp. 1028-1035. ISSN 1310-2818 http://doi.org/10.1080/13102818.2018.1460622 doi:10.1080/13102818.2018.1460622 |
| spellingShingle | Franco Molina, Moisés Armides Santana Krimskaya, Silvia Elena Coronado Cerda, Erika Evangelina Hernández Luna, Carlos Eduardo Zárate Triviño, Diana Ginette Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Salazar, María del Carmen Tamez Guerra, Reyes Silvestre Rodríguez Padilla, Cristina Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| title_full | Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| title_fullStr | Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| title_full_unstemmed | Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| title_short | Increase of the antitumour efficacy of the biocompound IMMUNEPOTENT CRP by enzymatic treatment |
| title_sort | increase of the antitumour efficacy of the biocompound immunepotent crp by enzymatic treatment |
| url | http://eprints.uanl.mx/16182/1/97.pdf |
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