| Summary: | Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease asbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to2016.Molecularidentificationwasperformedbysequenceanalysisofthepartial calmodulin gene. In vitro antifungal susceptibility to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), fluconazole (FLC), terbinafine (TRB), caspofungin (CSF), anidulafungin (ANF), and micafungin (MCF) was evaluated. Thirty-eight isolates of S. schenckii complexweredividedintofivesupportedcladesinaphylogenetictree. Thepredominantclinicalform waslymphocutaneous(92.3%),fixedcutaneous(5.1%),anddisseminated(2.5%). Terbinafineexhibited the best in vitro antifungal activity, while fluconazole was ineffective against Sporothrix schenckii complex. Our results showed diverse geographic distribution of clinical isolates in eight states; definitive identification was done by CAL gen PCR-sequencing. In Mexico, S. schenckii is considered to be an etiological agent of human sporotrichosis cases, and lymphocutaneous is the most prevalent form of the disease. This study revealed four clades of S. schenckii sensu stricto by phylogenetic analysis. Furthermore, we report one case of S. globosa isolated from human origin from the North of Mexico.
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