Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico
Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease asbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
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2018
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| Acceso en línea: | http://eprints.uanl.mx/16106/1/80.pdf |
| _version_ | 1824414500543004672 |
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| author | Rojas, Olga Bonifaz, Alexandro Campos, Christian Treviño Rangel, Rogelio de Jesús González Álvarez, Rafael González González, Gloria María |
| author_facet | Rojas, Olga Bonifaz, Alexandro Campos, Christian Treviño Rangel, Rogelio de Jesús González Álvarez, Rafael González González, Gloria María |
| author_sort | Rojas, Olga |
| collection | Repositorio Institucional |
| description | Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease asbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to2016.Molecularidentificationwasperformedbysequenceanalysisofthepartial calmodulin gene. In vitro antifungal susceptibility to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), fluconazole (FLC), terbinafine (TRB), caspofungin (CSF), anidulafungin (ANF), and micafungin (MCF) was evaluated. Thirty-eight isolates of S. schenckii complexweredividedintofivesupportedcladesinaphylogenetictree. Thepredominantclinicalform waslymphocutaneous(92.3%),fixedcutaneous(5.1%),anddisseminated(2.5%). Terbinafineexhibited the best in vitro antifungal activity, while fluconazole was ineffective against Sporothrix schenckii complex. Our results showed diverse geographic distribution of clinical isolates in eight states; definitive identification was done by CAL gen PCR-sequencing. In Mexico, S. schenckii is considered to be an etiological agent of human sporotrichosis cases, and lymphocutaneous is the most prevalent form of the disease. This study revealed four clades of S. schenckii sensu stricto by phylogenetic analysis. Furthermore, we report one case of S. globosa isolated from human origin from the North of Mexico. |
| format | Article |
| id | eprints-16106 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-161062024-03-05T17:51:45Z http://eprints.uanl.mx/16106/ Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico Rojas, Olga Bonifaz, Alexandro Campos, Christian Treviño Rangel, Rogelio de Jesús González Álvarez, Rafael González González, Gloria María QR Microbiología Abstract:SporotrichosisisasubcutaneousmycosiscausedbySporothrixschenckiicomplex. Thedisease asbeenreportedworldwide.However,theincidenceoftheetiologicalagentvariesinitsgeographic distribution. We studied 39 clinical isolates of Sporothrix schenckii from diverse regions in Mexico, collectedfrom1998to2016.Molecularidentificationwasperformedbysequenceanalysisofthepartial calmodulin gene. In vitro antifungal susceptibility to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), fluconazole (FLC), terbinafine (TRB), caspofungin (CSF), anidulafungin (ANF), and micafungin (MCF) was evaluated. Thirty-eight isolates of S. schenckii complexweredividedintofivesupportedcladesinaphylogenetictree. Thepredominantclinicalform waslymphocutaneous(92.3%),fixedcutaneous(5.1%),anddisseminated(2.5%). Terbinafineexhibited the best in vitro antifungal activity, while fluconazole was ineffective against Sporothrix schenckii complex. Our results showed diverse geographic distribution of clinical isolates in eight states; definitive identification was done by CAL gen PCR-sequencing. In Mexico, S. schenckii is considered to be an etiological agent of human sporotrichosis cases, and lymphocutaneous is the most prevalent form of the disease. This study revealed four clades of S. schenckii sensu stricto by phylogenetic analysis. Furthermore, we report one case of S. globosa isolated from human origin from the North of Mexico. 2018-07-20 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16106/1/80.pdf http://eprints.uanl.mx/16106/1.haspreviewThumbnailVersion/80.pdf Rojas, Olga y Bonifaz, Alexandro y Campos, Christian y Treviño Rangel, Rogelio de Jesús y González Álvarez, Rafael y González González, Gloria María (2018) Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico. Journal of Fungi, 4 (3). pp. 1-9. ISSN 2309-608X http://doi.org/10.3390/jof4030086 doi:10.3390/jof4030086 |
| spellingShingle | QR Microbiología Rojas, Olga Bonifaz, Alexandro Campos, Christian Treviño Rangel, Rogelio de Jesús González Álvarez, Rafael González González, Gloria María Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| title_full | Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| title_fullStr | Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| title_full_unstemmed | Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| title_short | Molecular Identification, Antifungal Susceptibility, and Geographic Origin of Clinical Strains of Sporothrix schenckii Complex in Mexico |
| title_sort | molecular identification antifungal susceptibility and geographic origin of clinical strains of sporothrix schenckii complex in mexico |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/16106/1/80.pdf |
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