Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice
Abstract: Background: Acaciafarnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capaci...
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| Format: | Article |
| Language: | English |
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2018
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| Online Access: | http://eprints.uanl.mx/16096/1/71.pdf |
| _version_ | 1824414496087605248 |
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| author | Delgadillo Puga, Claudia Cuchillo Hilario, Mario Navarro Ocaña, Arturo Medina Campos, Omar Noel Nieto Camacho, Antonio Ramírez Apan, Teresa López Tecpoyotl, Zenón Gerardo Díaz Martínez, Margarita Alvarez Izazaga, Marsela Alejandra Cruz Martínez, Yessica Rosalina Sánchez Quezada, Vanessa Gómez, Francisco Enrique Torre Villalvazo, Iván Furuzawa Carballeda, Janette Camacho Corona, María del Rayo Pedraza Chaverri, José |
| author_facet | Delgadillo Puga, Claudia Cuchillo Hilario, Mario Navarro Ocaña, Arturo Medina Campos, Omar Noel Nieto Camacho, Antonio Ramírez Apan, Teresa López Tecpoyotl, Zenón Gerardo Díaz Martínez, Margarita Alvarez Izazaga, Marsela Alejandra Cruz Martínez, Yessica Rosalina Sánchez Quezada, Vanessa Gómez, Francisco Enrique Torre Villalvazo, Iván Furuzawa Carballeda, Janette Camacho Corona, María del Rayo Pedraza Chaverri, José |
| author_sort | Delgadillo Puga, Claudia |
| collection | Repositorio Institucional |
| description | Abstract: Background: Acaciafarnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacitytocurbtheinflammationprocessaswellastodown-regulatethepro-inflammatorymediators. Methods: Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessmentofanti-inflammatorypropertieswasmadebytheearedemainducedmodelinCD-1mice andMPOactivityassay. Likewise,histologicalanalysis,IL-1β,IL-6,IL-10,TNF-α,COXmeasurements plus nitrite and immunohistochemistry analysis were carried out. Results: Methyl gallate, gallic acid,galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays. Conclusion: All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents.
Keywords: Acacia farnesiana pods; antioxidant and anti-inflammatory activities; bioactive compounds; polyphenols |
| format | Article |
| id | eprints-16096 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-160962024-03-05T20:10:38Z http://eprints.uanl.mx/16096/ Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice Delgadillo Puga, Claudia Cuchillo Hilario, Mario Navarro Ocaña, Arturo Medina Campos, Omar Noel Nieto Camacho, Antonio Ramírez Apan, Teresa López Tecpoyotl, Zenón Gerardo Díaz Martínez, Margarita Alvarez Izazaga, Marsela Alejandra Cruz Martínez, Yessica Rosalina Sánchez Quezada, Vanessa Gómez, Francisco Enrique Torre Villalvazo, Iván Furuzawa Carballeda, Janette Camacho Corona, María del Rayo Pedraza Chaverri, José QD Química Abstract: Background: Acaciafarnesiana (AF) pods have been traditionally used to treat dyspepsia, diarrhea and topically for dermal inflammation. Main objectives: (1) investigate the antioxidant activity and protection against oxidative-induced damage of six extracts from AF pods and (2) their capacitytocurbtheinflammationprocessaswellastodown-regulatethepro-inflammatorymediators. Methods: Five organic extracts (chloroformic, hexanic, ketonic, methanolic, methanolic:aqueous and one aqueous extract) were obtained and analyzed by UPLC-ESI-Q-oa/TOF-MS. Antioxidant activity (DPPH•, ORAC and FRAP assays) and lipid peroxidation (TBARS assay) were performed. Assessmentofanti-inflammatorypropertieswasmadebytheearedemainducedmodelinCD-1mice andMPOactivityassay. Likewise,histologicalanalysis,IL-1β,IL-6,IL-10,TNF-α,COXmeasurements plus nitrite and immunohistochemistry analysis were carried out. Results: Methyl gallate, gallic acid,galloyl glucose isomer 1, galloyl glucose isomer 2, galloyl glucose isomer 3, digalloyl glucose isomer 1, digalloyl glucose isomer 2, digalloyl glucose isomer 3, digalloyl glucose isomer 4, hydroxytyrosol acetate, quinic acid, and caffeoylmalic acid were identified. Both organic and aqueous extracts displayed antioxidant activity. All extracts exhibited a positive effect on the interleukins, COX and immunohistochemistry assays. Conclusion: All AF pod extracts can be effective as antioxidant and topical anti-inflammatory agents. Keywords: Acacia farnesiana pods; antioxidant and anti-inflammatory activities; bioactive compounds; polyphenols 2018-09-18 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/16096/1/71.pdf http://eprints.uanl.mx/16096/1.haspreviewThumbnailVersion/71.pdf Delgadillo Puga, Claudia y Cuchillo Hilario, Mario y Navarro Ocaña, Arturo y Medina Campos, Omar Noel y Nieto Camacho, Antonio y Ramírez Apan, Teresa y López Tecpoyotl, Zenón Gerardo y Díaz Martínez, Margarita y Alvarez Izazaga, Marsela Alejandra y Cruz Martínez, Yessica Rosalina y Sánchez Quezada, Vanessa y Gómez, Francisco Enrique y Torre Villalvazo, Iván y Furuzawa Carballeda, Janette y Camacho Corona, María del Rayo y Pedraza Chaverri, José (2018) Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice. Molecules, 23 (9). pp. 1-21. ISSN 1420-3049 http://doi.org/10.3390/molecules23092386 doi:10.3390/molecules23092386 |
| spellingShingle | QD Química Delgadillo Puga, Claudia Cuchillo Hilario, Mario Navarro Ocaña, Arturo Medina Campos, Omar Noel Nieto Camacho, Antonio Ramírez Apan, Teresa López Tecpoyotl, Zenón Gerardo Díaz Martínez, Margarita Alvarez Izazaga, Marsela Alejandra Cruz Martínez, Yessica Rosalina Sánchez Quezada, Vanessa Gómez, Francisco Enrique Torre Villalvazo, Iván Furuzawa Carballeda, Janette Camacho Corona, María del Rayo Pedraza Chaverri, José Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| title_full | Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| title_fullStr | Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| title_full_unstemmed | Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| title_short | Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice |
| title_sort | phenolic compounds in organic and aqueous extracts from acacia farnesiana pods analyzed by ulps esi q oa tof ms in vitro antioxidant activity and anti inflammatory response in cd 1 mice |
| topic | QD Química |
| url | http://eprints.uanl.mx/16096/1/71.pdf |
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