Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly from patients’ skin biop...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/15971/1/44.pdf |
| _version_ | 1824414449749983232 |
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| author | Benjak, Andrej Avanzi, Charlotte Singh, Pushpendra Loiseau, Chloé Girma, Selfu Busso, Philippe Fontes, Amanda N. Brum Miyamoto, Yuji Namisato, Masako Bobosha, Kidist Salgado, Claudio G. Da Silva, Moisés B. Bouth, Raquel C. Frade, Marco A. C. Filho, Fred Bernardes Barreto, Josafá G. Nery, José A. C. Bührer Sékula, Samira Lupien, Andréanne Al Samie, Abdul R. Al Qubati, Yasin Alkubati, Abdul S. Bretzel, Gisela Vera Cabrera, Lucio Sakho, Fatoumata Johnson, Christian R. Kodio, Mamoudou Fomba, Abdoulaye Sow, Samba O. Gado, Moussa Konaté, Ousmane Stefani, Mariane M. A. Penna, Gerson O. Suffys, Philip N. Sarno, Euzenir Nunes Moraes, Milton O. Rosa, Patricia S. Baptista, Ida M. F. Dias Spencer, John S. Aseffa, Abraham Matsuoka, Masanori Kai, Masanori Cole, Stewart T. |
| author_facet | Benjak, Andrej Avanzi, Charlotte Singh, Pushpendra Loiseau, Chloé Girma, Selfu Busso, Philippe Fontes, Amanda N. Brum Miyamoto, Yuji Namisato, Masako Bobosha, Kidist Salgado, Claudio G. Da Silva, Moisés B. Bouth, Raquel C. Frade, Marco A. C. Filho, Fred Bernardes Barreto, Josafá G. Nery, José A. C. Bührer Sékula, Samira Lupien, Andréanne Al Samie, Abdul R. Al Qubati, Yasin Alkubati, Abdul S. Bretzel, Gisela Vera Cabrera, Lucio Sakho, Fatoumata Johnson, Christian R. Kodio, Mamoudou Fomba, Abdoulaye Sow, Samba O. Gado, Moussa Konaté, Ousmane Stefani, Mariane M. A. Penna, Gerson O. Suffys, Philip N. Sarno, Euzenir Nunes Moraes, Milton O. Rosa, Patricia S. Baptista, Ida M. F. Dias Spencer, John S. Aseffa, Abraham Matsuoka, Masanori Kai, Masanori Cole, Stewart T. |
| author_sort | Benjak, Andrej |
| collection | Repositorio Institucional |
| description | Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae.
Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still
reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly from
patients’ skin biopsies using a customized protocol. Comparative and phylogenetic analysis of 154
genomes from 25 countries provides insight into evolution and antimicrobial resistance, uncovering lineages and phylogeographic trends, with the most ancestral strains linked to the Far East.
In addition to known MDT-resistance mutations, we detect other mutations associated with
antibiotic resistance, and retrace a potential stepwise emergence of extensive drug resistance in
the pre-MDT era. Some of the previously undescribed mutations occur in genes that are apparently subject to positive selection, and two of these (ribD, fadD9) are restricted to drug-resistant
strains. Finally, nonsense mutations in the nth excision repair gene are associated with greater
sequence diversity and drug resistance. |
| format | Article |
| id | eprints-15971 |
| institution | UANL |
| language | English |
| publishDate | 2018 |
| record_format | eprints |
| spelling | eprints-159712024-12-10T15:29:28Z http://eprints.uanl.mx/15971/ Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae Benjak, Andrej Avanzi, Charlotte Singh, Pushpendra Loiseau, Chloé Girma, Selfu Busso, Philippe Fontes, Amanda N. Brum Miyamoto, Yuji Namisato, Masako Bobosha, Kidist Salgado, Claudio G. Da Silva, Moisés B. Bouth, Raquel C. Frade, Marco A. C. Filho, Fred Bernardes Barreto, Josafá G. Nery, José A. C. Bührer Sékula, Samira Lupien, Andréanne Al Samie, Abdul R. Al Qubati, Yasin Alkubati, Abdul S. Bretzel, Gisela Vera Cabrera, Lucio Sakho, Fatoumata Johnson, Christian R. Kodio, Mamoudou Fomba, Abdoulaye Sow, Samba O. Gado, Moussa Konaté, Ousmane Stefani, Mariane M. A. Penna, Gerson O. Suffys, Philip N. Sarno, Euzenir Nunes Moraes, Milton O. Rosa, Patricia S. Baptista, Ida M. F. Dias Spencer, John S. Aseffa, Abraham Matsuoka, Masanori Kai, Masanori Cole, Stewart T. RL Dermatología Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly from patients’ skin biopsies using a customized protocol. Comparative and phylogenetic analysis of 154 genomes from 25 countries provides insight into evolution and antimicrobial resistance, uncovering lineages and phylogeographic trends, with the most ancestral strains linked to the Far East. In addition to known MDT-resistance mutations, we detect other mutations associated with antibiotic resistance, and retrace a potential stepwise emergence of extensive drug resistance in the pre-MDT era. Some of the previously undescribed mutations occur in genes that are apparently subject to positive selection, and two of these (ribD, fadD9) are restricted to drug-resistant strains. Finally, nonsense mutations in the nth excision repair gene are associated with greater sequence diversity and drug resistance. 2018 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15971/1/44.pdf http://eprints.uanl.mx/15971/1.haspreviewThumbnailVersion/44.pdf Benjak, Andrej y Avanzi, Charlotte y Singh, Pushpendra y Loiseau, Chloé y Girma, Selfu y Busso, Philippe y Fontes, Amanda N. Brum y Miyamoto, Yuji y Namisato, Masako y Bobosha, Kidist y Salgado, Claudio G. y Da Silva, Moisés B. y Bouth, Raquel C. y Frade, Marco A. C. y Filho, Fred Bernardes y Barreto, Josafá G. y Nery, José A. C. y Bührer Sékula, Samira y Lupien, Andréanne y Al Samie, Abdul R. y Al Qubati, Yasin y Alkubati, Abdul S. y Bretzel, Gisela y Vera Cabrera, Lucio y Sakho, Fatoumata y Johnson, Christian R. y Kodio, Mamoudou y Fomba, Abdoulaye y Sow, Samba O. y Gado, Moussa y Konaté, Ousmane y Stefani, Mariane M. A. y Penna, Gerson O. y Suffys, Philip N. y Sarno, Euzenir Nunes y Moraes, Milton O. y Rosa, Patricia S. y Baptista, Ida M. F. Dias y Spencer, John S. y Aseffa, Abraham y Matsuoka, Masanori y Kai, Masanori y Cole, Stewart T. (2018) Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae. Nature Communications, 9 (1). pp. 1-11. ISSN 2041-1723 http://doi.org/10.1038/s41467-017-02576-z doi:10.1038/s41467-017-02576-z |
| spellingShingle | RL Dermatología Benjak, Andrej Avanzi, Charlotte Singh, Pushpendra Loiseau, Chloé Girma, Selfu Busso, Philippe Fontes, Amanda N. Brum Miyamoto, Yuji Namisato, Masako Bobosha, Kidist Salgado, Claudio G. Da Silva, Moisés B. Bouth, Raquel C. Frade, Marco A. C. Filho, Fred Bernardes Barreto, Josafá G. Nery, José A. C. Bührer Sékula, Samira Lupien, Andréanne Al Samie, Abdul R. Al Qubati, Yasin Alkubati, Abdul S. Bretzel, Gisela Vera Cabrera, Lucio Sakho, Fatoumata Johnson, Christian R. Kodio, Mamoudou Fomba, Abdoulaye Sow, Samba O. Gado, Moussa Konaté, Ousmane Stefani, Mariane M. A. Penna, Gerson O. Suffys, Philip N. Sarno, Euzenir Nunes Moraes, Milton O. Rosa, Patricia S. Baptista, Ida M. F. Dias Spencer, John S. Aseffa, Abraham Matsuoka, Masanori Kai, Masanori Cole, Stewart T. Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| title_full | Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| title_fullStr | Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| title_full_unstemmed | Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| title_short | Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae |
| title_sort | phylogenomics and antimicrobial resistance of the leprosy bacillus mycobacterium leprae |
| topic | RL Dermatología |
| url | http://eprints.uanl.mx/15971/1/44.pdf |
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