Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination
Background: Collection of the black fly vectors of onchocerciasis worldwide relies upon human landing collections. Recent studies have suggested that the Esperanza Window Trap baited with a human scent lure and CO2 had the potential to replace human hosts for the collection of Simulium ochraceum sen...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/15254/1/362.pdf |
| _version_ | 1824414302298177536 |
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| author | Mutuku, Francis Rodríguez Pérez, Mario Alberto Adeleke, Monsuru Adebayo Rodríguez Luna, Isabel C. Cupp, Eddie W. Unnasch, Thomas R. |
| author_facet | Mutuku, Francis Rodríguez Pérez, Mario Alberto Adeleke, Monsuru Adebayo Rodríguez Luna, Isabel C. Cupp, Eddie W. Unnasch, Thomas R. |
| author_sort | Mutuku, Francis |
| collection | Repositorio Institucional |
| description | Background: Collection of the black fly vectors of onchocerciasis worldwide relies upon human landing collections. Recent studies have suggested that the Esperanza Window Trap baited with a human scent lure and CO2 had the potential to replace human hosts for the collection of Simulium ochraceum sensu lato in Southern Chiapas focus, Mexico. The feasibility of utilizing these traps in a community-based approach for the collection of S. ochraceum s.l. was evaluated.
Methodology/Principal findings: Local residents of a formerly endemic extra-sentinel community for onchocerciasis were trained to carry out collections using the traps. The residents operated the traps over a 60-day period and conducted parallel landing collections, resulting in a total of 28,397 vector black flies collected. None of the flies collected were found
to contain parasite DNA when tested by a polymerase chain reaction assay targeting a parasite specific sequence, resulting in a point estimate of infection in the vectors of zero, with an upper bound of the 95% confidence interval 0.13 per 2,000. This meets the accepted criterion for demonstrating an interruption of parasite transmission.
Conclusions/Significance: These data demonstrate that Esperanza Window Traps may be effectively operated by minimally trained residents of formerly endemic communities, resulting in the collection of sufficient numbers of flies to verify transmission interruption of onchocerciasis. The traps represent a viable alternative to using humans as hosts for the collection of vector flies as part of the verification of onchocerciasis elimination. |
| format | Article |
| id | eprints-15254 |
| institution | UANL |
| language | English |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | eprints |
| spelling | eprints-152542020-06-08T20:34:12Z http://eprints.uanl.mx/15254/ Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination Mutuku, Francis Rodríguez Pérez, Mario Alberto Adeleke, Monsuru Adebayo Rodríguez Luna, Isabel C. Cupp, Eddie W. Unnasch, Thomas R. RA Aspectos Públicos de la Medicina Background: Collection of the black fly vectors of onchocerciasis worldwide relies upon human landing collections. Recent studies have suggested that the Esperanza Window Trap baited with a human scent lure and CO2 had the potential to replace human hosts for the collection of Simulium ochraceum sensu lato in Southern Chiapas focus, Mexico. The feasibility of utilizing these traps in a community-based approach for the collection of S. ochraceum s.l. was evaluated. Methodology/Principal findings: Local residents of a formerly endemic extra-sentinel community for onchocerciasis were trained to carry out collections using the traps. The residents operated the traps over a 60-day period and conducted parallel landing collections, resulting in a total of 28,397 vector black flies collected. None of the flies collected were found to contain parasite DNA when tested by a polymerase chain reaction assay targeting a parasite specific sequence, resulting in a point estimate of infection in the vectors of zero, with an upper bound of the 95% confidence interval 0.13 per 2,000. This meets the accepted criterion for demonstrating an interruption of parasite transmission. Conclusions/Significance: These data demonstrate that Esperanza Window Traps may be effectively operated by minimally trained residents of formerly endemic communities, resulting in the collection of sufficient numbers of flies to verify transmission interruption of onchocerciasis. The traps represent a viable alternative to using humans as hosts for the collection of vector flies as part of the verification of onchocerciasis elimination. Public Library of Science 2014 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15254/1/362.pdf http://eprints.uanl.mx/15254/1.haspreviewThumbnailVersion/362.pdf Mutuku, Francis y Rodríguez Pérez, Mario Alberto y Adeleke, Monsuru Adebayo y Rodríguez Luna, Isabel C. y Cupp, Eddie W. y Unnasch, Thomas R. (2014) Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination. PloS Neglected tropical diseases, 8 (10). e3249. ISSN 1935-2727 http://doi.org/10.1371/journal.pntd.0003249 doi:10.1371/journal.pntd.0003249 |
| spellingShingle | RA Aspectos Públicos de la Medicina Mutuku, Francis Rodríguez Pérez, Mario Alberto Adeleke, Monsuru Adebayo Rodríguez Luna, Isabel C. Cupp, Eddie W. Unnasch, Thomas R. Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| title_full | Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| title_fullStr | Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| title_full_unstemmed | Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| title_short | Evaluation of a community-based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| title_sort | evaluation of a community based trapping program to collect simulium ochraceum sensu lato for verification of onchocerciasis elimination |
| topic | RA Aspectos Públicos de la Medicina |
| url | http://eprints.uanl.mx/15254/1/362.pdf |
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