Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia
Commercial culture media present interlot variations in biological activity.We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medi...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/15225/1/321.pdf |
| _version_ | 1824414290274156544 |
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| author | Vargas Villarreal, Javier Mata Cárdenas, Benito David Hernández García, Magda Elizabeth Garza González, Jesús Norberto De la Garza Salinas, Laura Hermila González Salazar, Francisco |
| author_facet | Vargas Villarreal, Javier Mata Cárdenas, Benito David Hernández García, Magda Elizabeth Garza González, Jesús Norberto De la Garza Salinas, Laura Hermila González Salazar, Francisco |
| author_sort | Vargas Villarreal, Javier |
| collection | Repositorio Institucional |
| description | Commercial culture media present interlot variations in biological activity.We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen
or refrigerated. The objective of this work was to modify PEHPS medium to support the in vitro growth of Giardia lamblia. Inocula of 5 × 103 trophozoites/mL of G. lamblia were incubated at 36.5∘ C in modified PEHPS or TYI-S-33 medium. Then, the growths of the three Giardia strains in both media were compared. The logarithmic growth phase lasted 72 h; the mean yield of the strains ranged from 10.06 to 11.43 × 105 Giardia trophozoites/mL, and the range of duplication time in the three strains was from 5.67 to 6.06 in modified PEHPS medium. These growth characteristics were not significantly different from those obtained with TYI-S-33 medium. We conclude that modified PEHPS medium might be used for the axenic cultivation of G. lamblia. |
| format | Article |
| id | eprints-15225 |
| institution | UANL |
| language | English |
| publishDate | 2014 |
| record_format | eprints |
| spelling | eprints-152252024-12-11T15:28:04Z http://eprints.uanl.mx/15225/ Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia Vargas Villarreal, Javier Mata Cárdenas, Benito David Hernández García, Magda Elizabeth Garza González, Jesús Norberto De la Garza Salinas, Laura Hermila González Salazar, Francisco QR Microbiología TD Tecnología Ambiental, Ingeniería Sanitaria Commercial culture media present interlot variations in biological activity.We have previously designed a homemade and economic culture medium, PEHPS medium, for the axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. Trophozoites of amoebae and trichomonads grow well in this medium. Furthermore, the medium is stable for several months when stored frozen or refrigerated. The objective of this work was to modify PEHPS medium to support the in vitro growth of Giardia lamblia. Inocula of 5 × 103 trophozoites/mL of G. lamblia were incubated at 36.5∘ C in modified PEHPS or TYI-S-33 medium. Then, the growths of the three Giardia strains in both media were compared. The logarithmic growth phase lasted 72 h; the mean yield of the strains ranged from 10.06 to 11.43 × 105 Giardia trophozoites/mL, and the range of duplication time in the three strains was from 5.67 to 6.06 in modified PEHPS medium. These growth characteristics were not significantly different from those obtained with TYI-S-33 medium. We conclude that modified PEHPS medium might be used for the axenic cultivation of G. lamblia. 2014 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15225/1/321.pdf http://eprints.uanl.mx/15225/1.haspreviewThumbnailVersion/321.pdf Vargas Villarreal, Javier y Mata Cárdenas, Benito David y Hernández García, Magda Elizabeth y Garza González, Jesús Norberto y De la Garza Salinas, Laura Hermila y González Salazar, Francisco (2014) Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia. BioMed Research International, 2014. pp. 1-4. ISSN 2314-6133 http://doi.org/10.1155/2014/714173 doi:10.1155/2014/714173 |
| spellingShingle | QR Microbiología TD Tecnología Ambiental, Ingeniería Sanitaria Vargas Villarreal, Javier Mata Cárdenas, Benito David Hernández García, Magda Elizabeth Garza González, Jesús Norberto De la Garza Salinas, Laura Hermila González Salazar, Francisco Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| title_full | Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| title_fullStr | Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| title_full_unstemmed | Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| title_short | Modified PEHPS medium as an alternative for the in vitroCulture of Giardia lamblia |
| title_sort | modified pehps medium as an alternative for the in vitroculture of giardia lamblia |
| topic | QR Microbiología TD Tecnología Ambiental, Ingeniería Sanitaria |
| url | http://eprints.uanl.mx/15225/1/321.pdf |
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