Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv
The isolation and characterization of the lignan meso-dihydroguaiaretic acid (MDGA) from Larrea tridentata and its activity against Mycobacterial tuberculosis has been demonstrated, but no information regarding its mechanism of action has been documented. Therefore, in this study we carry out the g...
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| Format: | Article |
| Language: | English |
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2014
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| Online Access: | http://eprints.uanl.mx/15122/1/233.pdf |
| _version_ | 1824414267861893120 |
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| author | Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Rivera, Gildardo Segura Cabrera, Aldo Garza González, Elvira Camacho Corona, María del Rayo |
| author_facet | Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Rivera, Gildardo Segura Cabrera, Aldo Garza González, Elvira Camacho Corona, María del Rayo |
| author_sort | Clemente Soto, Aldo Francisco |
| collection | Repositorio Institucional |
| description | The isolation and characterization of the lignan meso-dihydroguaiaretic acid (MDGA) from Larrea tridentata and its activity against Mycobacterial tuberculosis has
been demonstrated, but no information regarding its mechanism of action has been documented. Therefore, in this study we carry out the gene expression from total RNA obtained from M. tuberculosis H37Rv treated with MDGA using microarray technology, which was validated by quantitative real time polymerase chain reaction. Results showed that the alpha subunit of coenzyme A transferase of M. tuberculosis H37Rv is present in both geraniol and 1-and 2-methylnaphthalene degradation pathways, which are targeted by MDGA. This assumption was supported by molecular docking which showed stable interaction between MDGA with the active site of the enzyme. We propose that inhibition of coenzyme A transferase of M. tuberculosis H37Rv results in the accumulation of geraniol and 1-and 2-methylnaphtalene inside bacteria, causing membrane destabilization and death of the pathogen. The natural product MDGA is thus an attractive template to develop new anti-tuberculosis drugs, because its target is different from those of known anti-tubercular agents. |
| format | Article |
| id | eprints-15122 |
| institution | UANL |
| language | English |
| publishDate | 2014 |
| record_format | eprints |
| spelling | eprints-151222021-05-10T15:32:29Z http://eprints.uanl.mx/15122/ Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Rivera, Gildardo Segura Cabrera, Aldo Garza González, Elvira Camacho Corona, María del Rayo QD Química QR Microbiología The isolation and characterization of the lignan meso-dihydroguaiaretic acid (MDGA) from Larrea tridentata and its activity against Mycobacterial tuberculosis has been demonstrated, but no information regarding its mechanism of action has been documented. Therefore, in this study we carry out the gene expression from total RNA obtained from M. tuberculosis H37Rv treated with MDGA using microarray technology, which was validated by quantitative real time polymerase chain reaction. Results showed that the alpha subunit of coenzyme A transferase of M. tuberculosis H37Rv is present in both geraniol and 1-and 2-methylnaphthalene degradation pathways, which are targeted by MDGA. This assumption was supported by molecular docking which showed stable interaction between MDGA with the active site of the enzyme. We propose that inhibition of coenzyme A transferase of M. tuberculosis H37Rv results in the accumulation of geraniol and 1-and 2-methylnaphtalene inside bacteria, causing membrane destabilization and death of the pathogen. The natural product MDGA is thus an attractive template to develop new anti-tuberculosis drugs, because its target is different from those of known anti-tubercular agents. 2014 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15122/1/233.pdf http://eprints.uanl.mx/15122/1.haspreviewThumbnailVersion/233.pdf Clemente Soto, Aldo Francisco y Balderas Rentería, Isaías y Rivera, Gildardo y Segura Cabrera, Aldo y Garza González, Elvira y Camacho Corona, María del Rayo (2014) Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv. Molecules, 19 (12). pp. 20170-20182. ISSN 1420-3049 http://doi.org/10.3390/molecules191220170 doi:10.3390/molecules191220170 |
| spellingShingle | QD Química QR Microbiología Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Rivera, Gildardo Segura Cabrera, Aldo Garza González, Elvira Camacho Corona, María del Rayo Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| title_full | Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| title_fullStr | Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| title_full_unstemmed | Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| title_short | Potential mechanism of action of meso-dihydroguaiaretic acid on mycobacterium tuberculosis H37Rv |
| title_sort | potential mechanism of action of meso dihydroguaiaretic acid on mycobacterium tuberculosis h37rv |
| topic | QD Química QR Microbiología |
| url | http://eprints.uanl.mx/15122/1/233.pdf |
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