mRNA levels of TLR4 and TLR5 are independent of H pylori
AIM: To determine if the presence H pylori or its virulence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS: For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma m...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
WJG Press
2008
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| Acceso en línea: | http://eprints.uanl.mx/15109/1/818.pdf |
| _version_ | 1824414262685073408 |
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| author | Garza González, Elvira Bocanegra García, Virgilio Bosques Padilla, Francisco Javier Flores Gutiérrez, Juan Pablo Moreno, Francisco Perez Perez, Guillermo Ignacio |
| author_facet | Garza González, Elvira Bocanegra García, Virgilio Bosques Padilla, Francisco Javier Flores Gutiérrez, Juan Pablo Moreno, Francisco Perez Perez, Guillermo Ignacio |
| author_sort | Garza González, Elvira |
| collection | Repositorio Institucional |
| description | AIM: To determine if the presence H pylori or its virulence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS: For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) and there were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological findings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition associated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro , are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5. |
| format | Article |
| id | eprints-15109 |
| institution | UANL |
| language | English |
| publishDate | 2008 |
| publisher | WJG Press |
| record_format | eprints |
| spelling | eprints-151092024-12-09T20:23:20Z http://eprints.uanl.mx/15109/ mRNA levels of TLR4 and TLR5 are independent of H pylori Garza González, Elvira Bocanegra García, Virgilio Bosques Padilla, Francisco Javier Flores Gutiérrez, Juan Pablo Moreno, Francisco Perez Perez, Guillermo Ignacio AIM: To determine if the presence H pylori or its virulence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS: For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) and there were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological findings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition associated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro , are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5. WJG Press 2008 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15109/1/818.pdf http://eprints.uanl.mx/15109/1.haspreviewThumbnailVersion/818.pdf Garza González, Elvira y Bocanegra García, Virgilio y Bosques Padilla, Francisco Javier y Flores Gutiérrez, Juan Pablo y Moreno, Francisco y Perez Perez, Guillermo Ignacio (2008) mRNA levels of TLR4 and TLR5 are independent of H pylori. World journal of gastroenterology, 14 (34). p. 5306. ISSN 1007-9327 http://doi.org/10.3748/wjg.14.5306 doi:10.3748/wjg.14.5306 |
| spellingShingle | Garza González, Elvira Bocanegra García, Virgilio Bosques Padilla, Francisco Javier Flores Gutiérrez, Juan Pablo Moreno, Francisco Perez Perez, Guillermo Ignacio mRNA levels of TLR4 and TLR5 are independent of H pylori |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | mRNA levels of TLR4 and TLR5 are independent of H pylori |
| title_full | mRNA levels of TLR4 and TLR5 are independent of H pylori |
| title_fullStr | mRNA levels of TLR4 and TLR5 are independent of H pylori |
| title_full_unstemmed | mRNA levels of TLR4 and TLR5 are independent of H pylori |
| title_short | mRNA levels of TLR4 and TLR5 are independent of H pylori |
| title_sort | mrna levels of tlr4 and tlr5 are independent of h pylori |
| url | http://eprints.uanl.mx/15109/1/818.pdf |
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