Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection
The endoplasmic reticulum (ER) is where the major histocompatibility complex (MHC) class I molecules are loaded with epitopes to cause an immune cellular response. Most of the protein antigens are degraded in the cytoplasm to amino acids and few epitopes reach the ER. Antigen targeting of this organ...
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2009
|
| Acceso en línea: | http://eprints.uanl.mx/15086/1/758.pdf |
| _version_ | 1824414253377912832 |
|---|---|
| author | Loera Arias, María de Jesús Martínez Pérez, Alejandra Guadalupe Barrera Hernández, A. Ibarra Obregón, E. R. González Saldívar, Gerardo Martínez Ortega, J. I. Rosas Taraco, Adrián Geovanni Villanueva Olivo, Arnulfo Esparza González, Sandra Cecilia Villatoro Hernández, Julio Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto |
| author_facet | Loera Arias, María de Jesús Martínez Pérez, Alejandra Guadalupe Barrera Hernández, A. Ibarra Obregón, E. R. González Saldívar, Gerardo Martínez Ortega, J. I. Rosas Taraco, Adrián Geovanni Villanueva Olivo, Arnulfo Esparza González, Sandra Cecilia Villatoro Hernández, Julio Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto |
| author_sort | Loera Arias, María de Jesús |
| collection | Repositorio Institucional |
| description | The endoplasmic reticulum (ER) is where the major histocompatibility complex (MHC) class I molecules are loaded with epitopes to cause an immune cellular response. Most of the protein antigens are degraded in the cytoplasm to amino acids and few epitopes reach the ER. Antigen targeting of this organelle by Calreticulin (CRT) fusion avoids this degradation and enhances the immune response. We constructed a recombinant adenovirus to express the E7 antigen with an ER-targeting signal peptide (SP) plus an ER retention signal (KDEL sequence). In cell-culture experiments we demonstrated that this new E7 antigen, SP-E7-KDEL, targeted the ER. Infection of mice with this recombinant adenovirus that expresses SP-E7-KDEL showed interferon induction and tumour-protection response, similar to that provided by an adenovirus expressing the E7 antigen fused to CRT. This work demonstrated that just by adding a SP and the KDEL sequence, antigens can be targeted and retained in the ER with a consequent enhancement of immune response and tumour protection. These results will have significant clinical applications. |
| format | Article |
| id | eprints-15086 |
| institution | UANL |
| language | English |
| publishDate | 2009 |
| record_format | eprints |
| spelling | eprints-150862024-03-05T16:14:59Z http://eprints.uanl.mx/15086/ Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection Loera Arias, María de Jesús Martínez Pérez, Alejandra Guadalupe Barrera Hernández, A. Ibarra Obregón, E. R. González Saldívar, Gerardo Martínez Ortega, J. I. Rosas Taraco, Adrián Geovanni Villanueva Olivo, Arnulfo Esparza González, Sandra Cecilia Villatoro Hernández, Julio Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto The endoplasmic reticulum (ER) is where the major histocompatibility complex (MHC) class I molecules are loaded with epitopes to cause an immune cellular response. Most of the protein antigens are degraded in the cytoplasm to amino acids and few epitopes reach the ER. Antigen targeting of this organelle by Calreticulin (CRT) fusion avoids this degradation and enhances the immune response. We constructed a recombinant adenovirus to express the E7 antigen with an ER-targeting signal peptide (SP) plus an ER retention signal (KDEL sequence). In cell-culture experiments we demonstrated that this new E7 antigen, SP-E7-KDEL, targeted the ER. Infection of mice with this recombinant adenovirus that expresses SP-E7-KDEL showed interferon induction and tumour-protection response, similar to that provided by an adenovirus expressing the E7 antigen fused to CRT. This work demonstrated that just by adding a SP and the KDEL sequence, antigens can be targeted and retained in the ER with a consequent enhancement of immune response and tumour protection. These results will have significant clinical applications. 2009 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15086/1/758.pdf http://eprints.uanl.mx/15086/1.haspreviewThumbnailVersion/758.pdf Loera Arias, María de Jesús y Martínez Pérez, Alejandra Guadalupe y Barrera Hernández, A. y Ibarra Obregón, E. R. y González Saldívar, Gerardo y Martínez Ortega, J. I. y Rosas Taraco, Adrián Geovanni y Villanueva Olivo, Arnulfo y Esparza González, Sandra Cecilia y Villatoro Hernández, Julio y Saucedo Cárdenas, Odila y Montes de Oca Luna, Roberto (2009) Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection. Journal of Cellular and Molecular Medicine, 14 (4). pp. 890-894. ISSN 15821838 http://doi.org/10.1111/j.1582-4934.2009.00934.x doi:10.1111/j.1582-4934.2009.00934.x |
| spellingShingle | Loera Arias, María de Jesús Martínez Pérez, Alejandra Guadalupe Barrera Hernández, A. Ibarra Obregón, E. R. González Saldívar, Gerardo Martínez Ortega, J. I. Rosas Taraco, Adrián Geovanni Villanueva Olivo, Arnulfo Esparza González, Sandra Cecilia Villatoro Hernández, Julio Saucedo Cárdenas, Odila Montes de Oca Luna, Roberto Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| title_full | Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| title_fullStr | Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| title_full_unstemmed | Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| title_short | Targeting and retention of HPV16 E7 to the endoplasmic reticulum enhances immune tumour protection |
| title_sort | targeting and retention of hpv16 e7 to the endoplasmic reticulum enhances immune tumour protection |
| url | http://eprints.uanl.mx/15086/1/758.pdf |
| work_keys_str_mv | AT loeraariasmariadejesus targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT martinezperezalejandraguadalupe targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT barrerahernandeza targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT ibarraobregoner targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT gonzalezsaldivargerardo targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT martinezortegaji targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT rosastaracoadriangeovanni targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT villanuevaolivoarnulfo targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT esparzagonzalezsandracecilia targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT villatorohernandezjulio targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT saucedocardenasodila targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection AT montesdeocalunaroberto targetingandretentionofhpv16e7totheendoplasmicreticulumenhancesimmunetumourprotection |