PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture
Background: Previous in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles (PVPcoated AgNPs) have antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal activity by preventing the interaction of H...
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| Format: | Article |
| Language: | English |
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2010
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| Online Access: | http://eprints.uanl.mx/15080/1/741.pdf |
| _version_ | 1824414250974576640 |
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| author | Lara Villegas, Humberto Herman Ixtepan Turrent, Liliana del Carmen Garza Treviño, Elsa Nancy Rodríguez Padilla, Cristina |
| author_facet | Lara Villegas, Humberto Herman Ixtepan Turrent, Liliana del Carmen Garza Treviño, Elsa Nancy Rodríguez Padilla, Cristina |
| author_sort | Lara Villegas, Humberto Herman |
| collection | Repositorio Institucional |
| description | Background: Previous in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles (PVPcoated AgNPs) have antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal activity by preventing the interaction of HIV-1 gp120 and cellular CD4, thereby inhibiting fusion or entry of the virus into the host cell. In this study, we evaluated the antiviral activity of PVP-coated AgNPs as a potential topical vaginal microbicide to prevent transmission of HIV-1 infection using human cervical culture, an in vitro model that simulates in vivo conditions. Results: When formulated into a non-spermicidal gel (Replens) at a concentration of 0.15 mg/mL, PVP-coated AgNPs prevented the transmission of cell-associated HIV-1 and cell-free HIV-1 isolates. Importantly, PVP-coated AgNPs were not toxic to the explant, even when the cervical tissues were exposed continuously to 0.15 mg/mL of PVP-coated AgNPs for 48 h. Only 1 min of PVP-coated AgNPs pretreatment to the explant was required to prevent transmission of HIV-1. Pre-treatment of the cervical explant with 0.15 mg/mL PVP-coated AgNPs for 20 min followed by extensive washing prevented the transmission of HIV-1 in this model for 48 h. Conclusions: A formulation of PVP-coated AgNPs homogenized in Replens gel acts rapidly to inhibit HIV-1 transmission after 1 min and offers long-lasting protection of the cervical tissue from infection for 48 h, with no evidence of cytotoxicity observed in the explants. Based on this data, PVP-coated AgNPs are a promising microbicidal candidate for use in topical vaginal/cervical agents to prevent HIV-1 transmission, and further research is warranted |
| format | Article |
| id | eprints-15080 |
| institution | UANL |
| language | English |
| publishDate | 2010 |
| record_format | eprints |
| spelling | eprints-150802024-12-11T15:27:14Z http://eprints.uanl.mx/15080/ PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture Lara Villegas, Humberto Herman Ixtepan Turrent, Liliana del Carmen Garza Treviño, Elsa Nancy Rodríguez Padilla, Cristina Background: Previous in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles (PVPcoated AgNPs) have antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal activity by preventing the interaction of HIV-1 gp120 and cellular CD4, thereby inhibiting fusion or entry of the virus into the host cell. In this study, we evaluated the antiviral activity of PVP-coated AgNPs as a potential topical vaginal microbicide to prevent transmission of HIV-1 infection using human cervical culture, an in vitro model that simulates in vivo conditions. Results: When formulated into a non-spermicidal gel (Replens) at a concentration of 0.15 mg/mL, PVP-coated AgNPs prevented the transmission of cell-associated HIV-1 and cell-free HIV-1 isolates. Importantly, PVP-coated AgNPs were not toxic to the explant, even when the cervical tissues were exposed continuously to 0.15 mg/mL of PVP-coated AgNPs for 48 h. Only 1 min of PVP-coated AgNPs pretreatment to the explant was required to prevent transmission of HIV-1. Pre-treatment of the cervical explant with 0.15 mg/mL PVP-coated AgNPs for 20 min followed by extensive washing prevented the transmission of HIV-1 in this model for 48 h. Conclusions: A formulation of PVP-coated AgNPs homogenized in Replens gel acts rapidly to inhibit HIV-1 transmission after 1 min and offers long-lasting protection of the cervical tissue from infection for 48 h, with no evidence of cytotoxicity observed in the explants. Based on this data, PVP-coated AgNPs are a promising microbicidal candidate for use in topical vaginal/cervical agents to prevent HIV-1 transmission, and further research is warranted 2010 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15080/1/741.pdf http://eprints.uanl.mx/15080/1.haspreviewThumbnailVersion/741.pdf Lara Villegas, Humberto Herman y Ixtepan Turrent, Liliana del Carmen y Garza Treviño, Elsa Nancy y Rodríguez Padilla, Cristina (2010) PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture. Journal of Nanobiotechnology, 8 (1). p. 15. ISSN 1477-3155 http://doi.org/10.1186/1477-3155-8-15 doi:10.1186/1477-3155-8-15 |
| spellingShingle | Lara Villegas, Humberto Herman Ixtepan Turrent, Liliana del Carmen Garza Treviño, Elsa Nancy Rodríguez Padilla, Cristina PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| title_full | PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| title_fullStr | PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| title_full_unstemmed | PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| title_short | PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture |
| title_sort | pvp coated silver nanoparticles block the transmission of cell free and cell associated hiv 1 in human cervical culture |
| url | http://eprints.uanl.mx/15080/1/741.pdf |
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