An XMRV Derived Retroviral Vector as a Tool for Gene Transfer
Background: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
BioMed Central Ltd.
2011
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| Acceso en línea: | http://eprints.uanl.mx/15064/1/691.pdf |
| _version_ | 1824414244275224576 |
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| author | Cervantes García, Daniel Rojas Martínez, Augusto Camerini, David |
| author_facet | Cervantes García, Daniel Rojas Martínez, Augusto Camerini, David |
| author_sort | Cervantes García, Daniel |
| collection | Repositorio Institucional |
| description | Background: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described. Findings: We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids. Conclusions: We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies. |
| format | Article |
| id | eprints-15064 |
| institution | UANL |
| language | English |
| publishDate | 2011 |
| publisher | BioMed Central Ltd. |
| record_format | eprints |
| spelling | eprints-150642022-04-04T20:24:58Z http://eprints.uanl.mx/15064/ An XMRV Derived Retroviral Vector as a Tool for Gene Transfer Cervantes García, Daniel Rojas Martínez, Augusto Camerini, David RC Medicina Interna, Psiquiatría, Neurología Background: Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described. Findings: We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids. Conclusions: We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies. BioMed Central Ltd. 2011 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15064/1/691.pdf http://eprints.uanl.mx/15064/1.haspreviewThumbnailVersion/691.pdf Cervantes García, Daniel y Rojas Martínez, Augusto y Camerini, David (2011) An XMRV Derived Retroviral Vector as a Tool for Gene Transfer. Virology journal, 8 (1). p. 284. ISSN 1743-422X http://doi.org/10.1186/1743-422X-8-284 doi:10.1186/1743-422X-8-284 |
| spellingShingle | RC Medicina Interna, Psiquiatría, Neurología Cervantes García, Daniel Rojas Martínez, Augusto Camerini, David An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_full | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_fullStr | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_full_unstemmed | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_short | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_sort | xmrv derived retroviral vector as a tool for gene transfer |
| topic | RC Medicina Interna, Psiquiatría, Neurología |
| url | http://eprints.uanl.mx/15064/1/691.pdf |
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