Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis

The efficacy of decoction in extracting mycobactericidal compounds from Flourensia cernua (Hojas´e) leaves and fractionation with solvents having ascending polarity was compared with that of (i) ethanol extraction by still maceration, extraction with a Soxhlet device, shake-assisted maceration, or u...

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Autores principales: Molina Salinas, Gloria María, Peña Rodríguez, Luis Manuel, Mata Cárdenas, Benito David, Escalante Erosa, Fabiola, González Hernández, Silvia, Torres de la Cruz, Víctor Manuel, Martínez Rodríguez, Herminia Guadalupe, Said Fernández, Salvador
Formato: Artículo
Lenguaje:inglés
Publicado: 2011
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Acceso en línea:http://eprints.uanl.mx/15063/1/690.pdf
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author Molina Salinas, Gloria María
Peña Rodríguez, Luis Manuel
Mata Cárdenas, Benito David
Escalante Erosa, Fabiola
González Hernández, Silvia
Torres de la Cruz, Víctor Manuel
Martínez Rodríguez, Herminia Guadalupe
Said Fernández, Salvador
author_facet Molina Salinas, Gloria María
Peña Rodríguez, Luis Manuel
Mata Cárdenas, Benito David
Escalante Erosa, Fabiola
González Hernández, Silvia
Torres de la Cruz, Víctor Manuel
Martínez Rodríguez, Herminia Guadalupe
Said Fernández, Salvador
author_sort Molina Salinas, Gloria María
collection Repositorio Institucional
description The efficacy of decoction in extracting mycobactericidal compounds from Flourensia cernua (Hojas´e) leaves and fractionation with solvents having ascending polarity was compared with that of (i) ethanol extraction by still maceration, extraction with a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration, followed by fractionation with n-hexane, ethyl acetate, and n-butanol; (ii) sequential extraction with n-hexane, ethyl acetate, and n-butanol, by still maceration, using a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration. The in vitro mycobactericidal activity of each preparation was measured against drug-sensitive (SMtb) and drug-resistant (RMtb) Mycobacterium tuberculosis strains. The results of which were expressed as absolute mycobactericidal activity (AMA). These data were normalized to the ΣAMA of the decoction fraction set. Although decoction was inactive, the anti-RMtb normalized ΣAMA (NAMA) of its fractions was comparable with the anti-RMtb NAMA of the still maceration extracts and significantly higher than the anti-SMtb and anti-RMtb NAMAs of every other ethanol extract and serial extract and fraction. Hexane extracted, from decoction, material having 55.17% and 92.62% of antituberculosis activity against SMtb and RMtb, respectively. Although the mycobactericidal activity of decoction is undetectable; its efficacy in extracting F. cernua active metabolites against M. tuberculosis is substantially greater than almost all pharmacognostic methods.
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spelling eprints-150632020-06-11T19:14:09Z http://eprints.uanl.mx/15063/ Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis Molina Salinas, Gloria María Peña Rodríguez, Luis Manuel Mata Cárdenas, Benito David Escalante Erosa, Fabiola González Hernández, Silvia Torres de la Cruz, Víctor Manuel Martínez Rodríguez, Herminia Guadalupe Said Fernández, Salvador QR Microbiología The efficacy of decoction in extracting mycobactericidal compounds from Flourensia cernua (Hojas´e) leaves and fractionation with solvents having ascending polarity was compared with that of (i) ethanol extraction by still maceration, extraction with a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration, followed by fractionation with n-hexane, ethyl acetate, and n-butanol; (ii) sequential extraction with n-hexane, ethyl acetate, and n-butanol, by still maceration, using a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration. The in vitro mycobactericidal activity of each preparation was measured against drug-sensitive (SMtb) and drug-resistant (RMtb) Mycobacterium tuberculosis strains. The results of which were expressed as absolute mycobactericidal activity (AMA). These data were normalized to the ΣAMA of the decoction fraction set. Although decoction was inactive, the anti-RMtb normalized ΣAMA (NAMA) of its fractions was comparable with the anti-RMtb NAMA of the still maceration extracts and significantly higher than the anti-SMtb and anti-RMtb NAMAs of every other ethanol extract and serial extract and fraction. Hexane extracted, from decoction, material having 55.17% and 92.62% of antituberculosis activity against SMtb and RMtb, respectively. Although the mycobactericidal activity of decoction is undetectable; its efficacy in extracting F. cernua active metabolites against M. tuberculosis is substantially greater than almost all pharmacognostic methods. 2011 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/15063/1/690.pdf http://eprints.uanl.mx/15063/1.haspreviewThumbnailVersion/690.pdf Molina Salinas, Gloria María y Peña Rodríguez, Luis Manuel y Mata Cárdenas, Benito David y Escalante Erosa, Fabiola y González Hernández, Silvia y Torres de la Cruz, Víctor Manuel y Martínez Rodríguez, Herminia Guadalupe y Said Fernández, Salvador (2011) Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis. Evidence-Based Complementary and Alternative Medicine, 2011. pp. 1-9. ISSN 1741-427X http://doi.org/10.1155/2011/782503 doi:10.1155/2011/782503
spellingShingle QR Microbiología
Molina Salinas, Gloria María
Peña Rodríguez, Luis Manuel
Mata Cárdenas, Benito David
Escalante Erosa, Fabiola
González Hernández, Silvia
Torres de la Cruz, Víctor Manuel
Martínez Rodríguez, Herminia Guadalupe
Said Fernández, Salvador
Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
title_full Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
title_fullStr Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
title_full_unstemmed Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
title_short Flourensia cernua: Hexane Extracts a Very Active Mycobactericidal Fraction from an Inactive Leaf Decoction against Pansensitive and Panresistant Mycobacterium tuberculosis
title_sort flourensia cernua hexane extracts a very active mycobactericidal fraction from an inactive leaf decoction against pansensitive and panresistant mycobacterium tuberculosis
topic QR Microbiología
url http://eprints.uanl.mx/15063/1/690.pdf
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