Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection
Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Molecular Diversity Preservation International
2015
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| Acceso en línea: | http://eprints.uanl.mx/14978/1/137.pdf |
| _version_ | 1824414210496397312 |
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| author | Aguilar Briseño, José Alberto Cruz Suárez, Lucía Elizabeth Sassi, Jean François Ricque Marie, Denis Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Padilla, Cristina Trejo Ávila, Laura M. |
| author_facet | Aguilar Briseño, José Alberto Cruz Suárez, Lucía Elizabeth Sassi, Jean François Ricque Marie, Denis Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Padilla, Cristina Trejo Ávila, Laura M. |
| author_sort | Aguilar Briseño, José Alberto |
| collection | Repositorio Institucional |
| description | Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in
humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti
cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals
against paramyxovirus infection but their mixture has no clear synergistic advantage. |
| format | Article |
| id | eprints-14978 |
| institution | UANL |
| language | English |
| publishDate | 2015 |
| publisher | Molecular Diversity Preservation International |
| record_format | eprints |
| spelling | eprints-149782021-07-19T14:49:47Z http://eprints.uanl.mx/14978/ Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection Aguilar Briseño, José Alberto Cruz Suárez, Lucía Elizabeth Sassi, Jean François Ricque Marie, Denis Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Padilla, Cristina Trejo Ávila, Laura M. Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The paramyxoviridae family includes pathogens causing morbidity and mortality worldwide in humans and animals, such as the Newcastle Disease Virus (NDV) in poultry. This study aims at determining the antiviral activity and mechanism of action in vitro of an ulvan (SP from the green seaweed Ulva clathrata), and of its mixture with a fucoidan (SP from Cladosiphon okamuranus), against La Sota NDV strain. The ulvan antiviral activity was tested using syncytia formation, exhibiting an IC50 of 0.1 μg/mL; ulvan had a better anti cell-cell spread effect than that previously shown for fucoidan, and inhibited cell-cell fusion via a direct effect on the F0 protein, but did not show any virucidal effect. The mixture of ulvan and fucoidan showed a greater anti-spread effect than SPs alone, but ulvan antagonizes the effect of fucoidan on the viral attachment/entry. Both SPs may be promising antivirals against paramyxovirus infection but their mixture has no clear synergistic advantage. Molecular Diversity Preservation International 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14978/1/137.pdf http://eprints.uanl.mx/14978/1.haspreviewThumbnailVersion/137.pdf Aguilar Briseño, José Alberto y Cruz Suárez, Lucía Elizabeth y Sassi, Jean François y Ricque Marie, Denis y Zapata Benavides, Pablo y Mendoza Gamboa, Edgar y Rodríguez Padilla, Cristina y Trejo Ávila, Laura M. (2015) Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection. Marine drugs, 13 (2). pp. 697-712. ISSN 1660-3397 http://doi.org/10.3390/md13020697 doi:10.3390/md13020697 |
| spellingShingle | Aguilar Briseño, José Alberto Cruz Suárez, Lucía Elizabeth Sassi, Jean François Ricque Marie, Denis Zapata Benavides, Pablo Mendoza Gamboa, Edgar Rodríguez Padilla, Cristina Trejo Ávila, Laura M. Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| title_full | Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| title_fullStr | Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| title_full_unstemmed | Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| title_short | Sulphated Polysaccharides from Ulva clathrata and Cladosiphon okamuranus Seaweeds both Inhibit Viral Attachment/Entry and Cell-Cell Fusion, in NDV Infection |
| title_sort | sulphated polysaccharides from ulva clathrata and cladosiphon okamuranus seaweeds both inhibit viral attachment entry and cell cell fusion in ndv infection |
| url | http://eprints.uanl.mx/14978/1/137.pdf |
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