Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus
Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial ag...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2015
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| Acceso en línea: | http://eprints.uanl.mx/14953/1/124.pdf |
| _version_ | 1824414202745323520 |
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| author | Favela Hernández, Juan Manuel de Jesús Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Garza González, Elvira Camacho Corona, María del Rayo |
| author_facet | Favela Hernández, Juan Manuel de Jesús Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Garza González, Elvira Camacho Corona, María del Rayo |
| author_sort | Favela Hernández, Juan Manuel de Jesús |
| collection | Repositorio Institucional |
| description | Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections
in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial agents to treat these infections, and natural products are a rich source of them. In previous studies, we reported that lignan 3′-demethoxy-6-Odemethylisoguaiacin, isolated and characterized from Larrea tridentate, showed the best
activity towards methicillin-resistant S. aureus. Thus, the aim of this study was to determine the potential molecular mechanism of the antibacterial activity of 3′-demethoxy-6-Odemethylisoguaiacin against methicillin-resistant S. aureus using microarray technology.
Results of microarray genome expression were validated by real-time polymerase chain reaction (RT-PCR). The genetic profile expression results showed that lignan 3′-demethoxy6-O-demethylisoguaiacin had activity on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This molecular mechanism is not present in any antibacterial commercial drug and could be a new target for the development of novel antibacterial agents. |
| format | Article |
| id | eprints-14953 |
| institution | UANL |
| language | English |
| publishDate | 2015 |
| record_format | eprints |
| spelling | eprints-149532019-05-17T14:44:29Z http://eprints.uanl.mx/14953/ Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus Favela Hernández, Juan Manuel de Jesús Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Garza González, Elvira Camacho Corona, María del Rayo Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial agents to treat these infections, and natural products are a rich source of them. In previous studies, we reported that lignan 3′-demethoxy-6-Odemethylisoguaiacin, isolated and characterized from Larrea tridentate, showed the best activity towards methicillin-resistant S. aureus. Thus, the aim of this study was to determine the potential molecular mechanism of the antibacterial activity of 3′-demethoxy-6-Odemethylisoguaiacin against methicillin-resistant S. aureus using microarray technology. Results of microarray genome expression were validated by real-time polymerase chain reaction (RT-PCR). The genetic profile expression results showed that lignan 3′-demethoxy6-O-demethylisoguaiacin had activity on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This molecular mechanism is not present in any antibacterial commercial drug and could be a new target for the development of novel antibacterial agents. 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14953/1/124.pdf http://eprints.uanl.mx/14953/1.haspreviewThumbnailVersion/124.pdf Favela Hernández, Juan Manuel de Jesús y Clemente Soto, Aldo Francisco y Balderas Rentería, Isaías y Garza González, Elvira y Camacho Corona, María del Rayo (2015) Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus. Molecules, 20 (7). pp. 12450-12458. ISSN 1420-3049 http://doi.org/10.3390/molecules200712450 doi:10.3390/molecules200712450 |
| spellingShingle | Favela Hernández, Juan Manuel de Jesús Clemente Soto, Aldo Francisco Balderas Rentería, Isaías Garza González, Elvira Camacho Corona, María del Rayo Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| title_full | Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| title_fullStr | Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| title_full_unstemmed | Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| title_short | Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus |
| title_sort | potential mechanism of action of 3 demethoxy 6 o demethyl isoguaiacin on methicillin resistant staphylococcus aureus |
| url | http://eprints.uanl.mx/14953/1/124.pdf |
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