Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the os...

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Autores principales: Del Angel Mosqueda, Casiano, Gutiérrez Puente, Yolanda, López Lozano, Ada Pricila, Romero Zavaleta, Ricardo Emmanuel, Mendiola Jiménez, Andrés, Medina de la Garza, Carlos Eduardo, Márquez M, Marcela, De la Garza Ramos, Myriam Angélica
Formato: Artículo
Lenguaje:inglés
Publicado: 2015
Acceso en línea:http://eprints.uanl.mx/14946/1/117.pdf
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author Del Angel Mosqueda, Casiano
Gutiérrez Puente, Yolanda
López Lozano, Ada Pricila
Romero Zavaleta, Ricardo Emmanuel
Mendiola Jiménez, Andrés
Medina de la Garza, Carlos Eduardo
Márquez M, Marcela
De la Garza Ramos, Myriam Angélica
author_facet Del Angel Mosqueda, Casiano
Gutiérrez Puente, Yolanda
López Lozano, Ada Pricila
Romero Zavaleta, Ricardo Emmanuel
Mendiola Jiménez, Andrés
Medina de la Garza, Carlos Eduardo
Márquez M, Marcela
De la Garza Ramos, Myriam Angélica
author_sort Del Angel Mosqueda, Casiano
collection Repositorio Institucional
description Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology.
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spelling eprints-149462025-07-23T17:00:27Z http://eprints.uanl.mx/14946/ Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro Del Angel Mosqueda, Casiano Gutiérrez Puente, Yolanda López Lozano, Ada Pricila Romero Zavaleta, Ricardo Emmanuel Mendiola Jiménez, Andrés Medina de la Garza, Carlos Eduardo Márquez M, Marcela De la Garza Ramos, Myriam Angélica Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology. 2015 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14946/1/117.pdf http://eprints.uanl.mx/14946/1.haspreviewThumbnailVersion/117.pdf Del Angel Mosqueda, Casiano y Gutiérrez Puente, Yolanda y López Lozano, Ada Pricila y Romero Zavaleta, Ricardo Emmanuel y Mendiola Jiménez, Andrés y Medina de la Garza, Carlos Eduardo y Márquez M, Marcela y De la Garza Ramos, Myriam Angélica (2015) Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro. Head & Face Medicine, 11 (1). ISSN 1746-160X http://doi.org/10.1186/s13005-015-0086-5 doi:10.1186/s13005-015-0086-5
spellingShingle Del Angel Mosqueda, Casiano
Gutiérrez Puente, Yolanda
López Lozano, Ada Pricila
Romero Zavaleta, Ricardo Emmanuel
Mendiola Jiménez, Andrés
Medina de la Garza, Carlos Eduardo
Márquez M, Marcela
De la Garza Ramos, Myriam Angélica
Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
title_full Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
title_fullStr Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
title_full_unstemmed Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
title_short Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
title_sort epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
url http://eprints.uanl.mx/14946/1/117.pdf
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