Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola
The bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18uC and 20uC, while no detectable amounts are present above 28uC. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activit...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
Public Library of Science
2012
|
| Acceso en línea: | http://eprints.uanl.mx/14928/1/590.PDF |
| _version_ | 1824414192633905152 |
|---|---|
| author | Skurnik, Mikael Aguilera, Selene De la Torre Zavala, Susana Hernández Flores, José Luis Murillo, Jesús Bravo, Jaime Alvarez Morales, Ariel |
| author_facet | Skurnik, Mikael Aguilera, Selene De la Torre Zavala, Susana Hernández Flores, José Luis Murillo, Jesús Bravo, Jaime Alvarez Morales, Ariel |
| author_sort | Skurnik, Mikael |
| collection | Repositorio Institucional |
| description | The bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18uC and 20uC, while no detectable amounts are present above 28uC. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activity from plant, mammalian and bacterial sources and causes a phenotypic requirement for arginine. To protect the cell from its own toxin, P. syringae pv. phaseolicola synthesizes a phaseolotoxin-resistant OCTase (ROCT). The ROCT is the product of the argK gene and is synthesized only under conditions leading to phaseolotoxin synthesis. The argK gene is included in a chromosomal fragment named Pht cluster, which contains genes involved in the synthesis of phaseolotoxin. The aim of the present work was to investigate the possible involvement of other genes included in the Pht cluster in the regulation of gene argK. We conducted transcriptional analyses of argK in several mutants unable to produce phaseolotoxin, transcriptional fusions and electrophoretic mobility shift assays, which allowed us to determine that genes phtABC, located within the Pht cluster, participate in the transcriptional repression of gene argK at temperatures not permissive for phaseolotoxin biosynthesis. This repression is mediated by a protein present in both toxigenic and nontoxigenic strains of P. syringae and in E. coli, and requires the coordinated participation of phtA, phtB and phtC products in order to carry out an efficient argK repression. |
| format | Article |
| id | eprints-14928 |
| institution | UANL |
| language | English |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | eprints |
| spelling | eprints-149282020-03-05T22:58:44Z http://eprints.uanl.mx/14928/ Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola Skurnik, Mikael Aguilera, Selene De la Torre Zavala, Susana Hernández Flores, José Luis Murillo, Jesús Bravo, Jaime Alvarez Morales, Ariel The bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18uC and 20uC, while no detectable amounts are present above 28uC. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activity from plant, mammalian and bacterial sources and causes a phenotypic requirement for arginine. To protect the cell from its own toxin, P. syringae pv. phaseolicola synthesizes a phaseolotoxin-resistant OCTase (ROCT). The ROCT is the product of the argK gene and is synthesized only under conditions leading to phaseolotoxin synthesis. The argK gene is included in a chromosomal fragment named Pht cluster, which contains genes involved in the synthesis of phaseolotoxin. The aim of the present work was to investigate the possible involvement of other genes included in the Pht cluster in the regulation of gene argK. We conducted transcriptional analyses of argK in several mutants unable to produce phaseolotoxin, transcriptional fusions and electrophoretic mobility shift assays, which allowed us to determine that genes phtABC, located within the Pht cluster, participate in the transcriptional repression of gene argK at temperatures not permissive for phaseolotoxin biosynthesis. This repression is mediated by a protein present in both toxigenic and nontoxigenic strains of P. syringae and in E. coli, and requires the coordinated participation of phtA, phtB and phtC products in order to carry out an efficient argK repression. Public Library of Science 2012 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14928/1/590.PDF http://eprints.uanl.mx/14928/1.haspreviewThumbnailVersion/590.PDF Skurnik, Mikael y Aguilera, Selene y De la Torre Zavala, Susana y Hernández Flores, José Luis y Murillo, Jesús y Bravo, Jaime y Alvarez Morales, Ariel (2012) Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola. PloS one, 7 (10). e46815. ISSN 1932-6203 doi:10.1371/journal.pone.0046815 |
| spellingShingle | Skurnik, Mikael Aguilera, Selene De la Torre Zavala, Susana Hernández Flores, José Luis Murillo, Jesús Bravo, Jaime Alvarez Morales, Ariel Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| title_full | Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| title_fullStr | Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| title_full_unstemmed | Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| title_short | Expression of the Gene for Resistance to Phaseolotoxin (argK) Depends on the Activity of Genes phtABC in Pseudomonas syringae pv. phaseolicola |
| title_sort | expression of the gene for resistance to phaseolotoxin argk depends on the activity of genes phtabc in pseudomonas syringae pv phaseolicola |
| url | http://eprints.uanl.mx/14928/1/590.PDF |
| work_keys_str_mv | AT skurnikmikael expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT aguileraselene expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT delatorrezavalasusana expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT hernandezfloresjoseluis expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT murillojesus expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT bravojaime expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola AT alvarezmoralesariel expressionofthegeneforresistancetophaseolotoxinargkdependsontheactivityofgenesphtabcinpseudomonassyringaepvphaseolicola |