Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children
Polyomaviruses are small circular DNA viruses associated with chronic infections and tumors in both human and animal hosts. Using an unbiased deep sequencing approach, we identified a novel, highly divergent polyomavirus, provisionally named MX polyomavirus (MXPyV), in stool samples from children. T...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2012
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| Online Access: | http://eprints.uanl.mx/14922/1/582.PDF |
| _version_ | 1824414190145634304 |
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| author | Ramqvist, Torbjörn Yu, Guixia Greninger, Alexander L. Isa, Pavel Phan, Tung G. Martínez, Miguel Angel De la Luz Sanchez, Maria Contreras, Juan Francisco Santos Preciado, José Ignacio Parsonnet, Julie Miller, Steve DeRisi, Joseph L. Delwart, Eric Arias Ortiz, Carlos Federico Chiu, Charles Y. |
| author_facet | Ramqvist, Torbjörn Yu, Guixia Greninger, Alexander L. Isa, Pavel Phan, Tung G. Martínez, Miguel Angel De la Luz Sanchez, Maria Contreras, Juan Francisco Santos Preciado, José Ignacio Parsonnet, Julie Miller, Steve DeRisi, Joseph L. Delwart, Eric Arias Ortiz, Carlos Federico Chiu, Charles Y. |
| author_sort | Ramqvist, Torbjörn |
| collection | Repositorio Institucional |
| description | Polyomaviruses are small circular DNA viruses associated with chronic infections and tumors in both human and animal hosts. Using an unbiased deep sequencing approach, we identified a novel, highly divergent polyomavirus, provisionally named MX polyomavirus (MXPyV), in stool samples from children. The ,5.0 kB viral genome exhibits little overall homology (,46% amino acid identity) to known polyomaviruses, and, due to phylogenetic variation among its individual proteins, cannot be placed in any existing taxonomic group. PCR-based screening detected MXPyV in 28 of 834 (3.4%) fecal samples collected from California, Mexico, and Chile, and 1 of 136 (0.74%) of respiratory samples from Mexico, but not in blood or urine samples from immunocompromised patients. By quantitative PCR, the measured titers of MXPyV in human stool at 10% (weight/volume) were as high as 15,075 copies. No association was found between the presence of MXPyV and diarrhea, although girls were more likely to shed MXPyV in the stool than boys (p = 0.012). In one child, viral shedding was observed in two stools obtained 91 days apart, raising the possibility of chronic infection by MXPyV. A multiple sequence alignment revealed that MXPyV is a closely related variant of the recently reported MWPyV and HPyV10 polyomaviruses. Further studies will be important to determine the association, if any, of MXPyV with disease in humans. |
| format | Article |
| id | eprints-14922 |
| institution | UANL |
| language | English |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | eprints |
| spelling | eprints-149222021-05-03T15:12:54Z http://eprints.uanl.mx/14922/ Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children Ramqvist, Torbjörn Yu, Guixia Greninger, Alexander L. Isa, Pavel Phan, Tung G. Martínez, Miguel Angel De la Luz Sanchez, Maria Contreras, Juan Francisco Santos Preciado, José Ignacio Parsonnet, Julie Miller, Steve DeRisi, Joseph L. Delwart, Eric Arias Ortiz, Carlos Federico Chiu, Charles Y. Polyomaviruses are small circular DNA viruses associated with chronic infections and tumors in both human and animal hosts. Using an unbiased deep sequencing approach, we identified a novel, highly divergent polyomavirus, provisionally named MX polyomavirus (MXPyV), in stool samples from children. The ,5.0 kB viral genome exhibits little overall homology (,46% amino acid identity) to known polyomaviruses, and, due to phylogenetic variation among its individual proteins, cannot be placed in any existing taxonomic group. PCR-based screening detected MXPyV in 28 of 834 (3.4%) fecal samples collected from California, Mexico, and Chile, and 1 of 136 (0.74%) of respiratory samples from Mexico, but not in blood or urine samples from immunocompromised patients. By quantitative PCR, the measured titers of MXPyV in human stool at 10% (weight/volume) were as high as 15,075 copies. No association was found between the presence of MXPyV and diarrhea, although girls were more likely to shed MXPyV in the stool than boys (p = 0.012). In one child, viral shedding was observed in two stools obtained 91 days apart, raising the possibility of chronic infection by MXPyV. A multiple sequence alignment revealed that MXPyV is a closely related variant of the recently reported MWPyV and HPyV10 polyomaviruses. Further studies will be important to determine the association, if any, of MXPyV with disease in humans. Public Library of Science 2012 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14922/1/582.PDF http://eprints.uanl.mx/14922/1.haspreviewThumbnailVersion/582.PDF Ramqvist, Torbjörn y Yu, Guixia y Greninger, Alexander L. y Isa, Pavel y Phan, Tung G. y Martínez, Miguel Angel y De la Luz Sanchez, Maria y Contreras, Juan Francisco y Santos Preciado, José Ignacio y Parsonnet, Julie y Miller, Steve y DeRisi, Joseph L. y Delwart, Eric y Arias Ortiz, Carlos Federico y Chiu, Charles Y. (2012) Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children. PloS one, 7 (11). e49449. ISSN 1932-6203 http://doi.org/10.1371/journal.pone.0049449 doi:10.1371/journal.pone.0049449 |
| spellingShingle | Ramqvist, Torbjörn Yu, Guixia Greninger, Alexander L. Isa, Pavel Phan, Tung G. Martínez, Miguel Angel De la Luz Sanchez, Maria Contreras, Juan Francisco Santos Preciado, José Ignacio Parsonnet, Julie Miller, Steve DeRisi, Joseph L. Delwart, Eric Arias Ortiz, Carlos Federico Chiu, Charles Y. Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| title_full | Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| title_fullStr | Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| title_full_unstemmed | Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| title_short | Discovery of a Novel Polyomavirus in Acute Diarrheal Samples from Children |
| title_sort | discovery of a novel polyomavirus in acute diarrheal samples from children |
| url | http://eprints.uanl.mx/14922/1/582.PDF |
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