Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine

Research on mesenchymal stem cells (MSCs) continues to progress rapidly. Nevertheless, the field faces several challenges, such as inherent cell heterogeneity and the absence of unique MSCs markers. Due to MSCs’ ability to differentiate into multiple tissues, these cells represent a promising tool...

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Main Authors: Pérez Silos, Vanessa, Camacho Morales, Alberto, Fuentes Mera, Lizeth
Format: Article
Language:English
Published: 2016
Subjects:
Online Access:http://eprints.uanl.mx/14866/1/75.pdf
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author Pérez Silos, Vanessa
Camacho Morales, Alberto
Fuentes Mera, Lizeth
author_facet Pérez Silos, Vanessa
Camacho Morales, Alberto
Fuentes Mera, Lizeth
author_sort Pérez Silos, Vanessa
collection Repositorio Institucional
description Research on mesenchymal stem cells (MSCs) continues to progress rapidly. Nevertheless, the field faces several challenges, such as inherent cell heterogeneity and the absence of unique MSCs markers. Due to MSCs’ ability to differentiate into multiple tissues, these cells represent a promising tool for new cell-based therapies. However, for tissue engineering applications, it is critical to start with a well-defined cell population. Additionally, evidence that MSCs subpopulations may also feature distinct characteristics and regeneration potential has arisen. In this report, we present an overview of the identification of MSCs based on the expression of several surface markers and their current tissue sources. We review the use of MSCs subpopulations in recent years and the main methodologies that have addressed their isolation, and we emphasize the most-used surface markers for selection, isolation, and characterization. Next, we discuss the osteogenic and chondrogenic differentiation from MSCs subpopulations. We conclude that MSCs subpopulation selection is not a minor concern because each subpopulation has particular potential for promoting the differentiation into osteoblasts and chondrocytes. The accurate selection of the subpopulation advances possibilities suitable for preclinical and clinical studies and determines the safest and most efficacious regeneration process.
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spelling eprints-148662020-05-08T13:14:07Z http://eprints.uanl.mx/14866/ Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine Pérez Silos, Vanessa Camacho Morales, Alberto Fuentes Mera, Lizeth RM Terapéutica, Farmacología Research on mesenchymal stem cells (MSCs) continues to progress rapidly. Nevertheless, the field faces several challenges, such as inherent cell heterogeneity and the absence of unique MSCs markers. Due to MSCs’ ability to differentiate into multiple tissues, these cells represent a promising tool for new cell-based therapies. However, for tissue engineering applications, it is critical to start with a well-defined cell population. Additionally, evidence that MSCs subpopulations may also feature distinct characteristics and regeneration potential has arisen. In this report, we present an overview of the identification of MSCs based on the expression of several surface markers and their current tissue sources. We review the use of MSCs subpopulations in recent years and the main methodologies that have addressed their isolation, and we emphasize the most-used surface markers for selection, isolation, and characterization. Next, we discuss the osteogenic and chondrogenic differentiation from MSCs subpopulations. We conclude that MSCs subpopulation selection is not a minor concern because each subpopulation has particular potential for promoting the differentiation into osteoblasts and chondrocytes. The accurate selection of the subpopulation advances possibilities suitable for preclinical and clinical studies and determines the safest and most efficacious regeneration process. 2016 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14866/1/75.pdf http://eprints.uanl.mx/14866/1.haspreviewThumbnailVersion/75.pdf Pérez Silos, Vanessa y Camacho Morales, Alberto y Fuentes Mera, Lizeth (2016) Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine. Stem Cells International, 2016. pp. 1-9. ISSN 1687-966X http://doi.org/10.1155/2016/3187491 doi:10.1155/2016/3187491
spellingShingle RM Terapéutica, Farmacología
Pérez Silos, Vanessa
Camacho Morales, Alberto
Fuentes Mera, Lizeth
Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
thumbnail https://rediab.uanl.mx/themes/sandal5/images/online.png
title Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
title_full Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
title_fullStr Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
title_full_unstemmed Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
title_short Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine
title_sort mesenchymal stem cells subpopulations application for orthopedic regenerative medicine
topic RM Terapéutica, Farmacología
url http://eprints.uanl.mx/14866/1/75.pdf
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