Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays
The tissue microarrays (TMAs) were first called multitumor block. In 1998 was described the current technique, that uses an innovated sampling method for more than 1,000 cylindrical paraffin tissue core biopsies in a single paraffin block. TMAs are now considered as a useful powerful research tool i...
| Autores principales: | , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2013
|
| Acceso en línea: | http://eprints.uanl.mx/14860/1/538.pdf |
| _version_ | 1824414166825304064 |
|---|---|
| author | García Garza, Rubén Rodríguez Vidales, Edgar Paolo Soto Domínguez, Adolfo |
| author_facet | García Garza, Rubén Rodríguez Vidales, Edgar Paolo Soto Domínguez, Adolfo |
| author_sort | García Garza, Rubén |
| collection | Repositorio Institucional |
| description | The tissue microarrays (TMAs) were first called multitumor block. In 1998 was described the current technique, that uses an innovated sampling method for more than 1,000 cylindrical paraffin tissue core biopsies in a single paraffin block. TMAs are now considered as a useful powerful research tool in Histology and Pathology laboratories, for the standardization of immunohistochemical techniques along with in situ hybridization. However, one disadvantage to its widespread use is the high cost of professional paraffin tissue punches, and the complexity in the development of homemade devices previously described in other studies. This study describes a step by step process to develop four different home-made devices made with materials that are common in hospitals and offices. These devices are useful in Histopathology laboratories to obtain paraffin blocks with until 360 samples of tissue, investing from two to fifteen dollars in the development of each device described. |
| format | Article |
| id | eprints-14860 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| record_format | eprints |
| spelling | eprints-148602020-09-15T12:39:17Z http://eprints.uanl.mx/14860/ Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays García Garza, Rubén Rodríguez Vidales, Edgar Paolo Soto Domínguez, Adolfo The tissue microarrays (TMAs) were first called multitumor block. In 1998 was described the current technique, that uses an innovated sampling method for more than 1,000 cylindrical paraffin tissue core biopsies in a single paraffin block. TMAs are now considered as a useful powerful research tool in Histology and Pathology laboratories, for the standardization of immunohistochemical techniques along with in situ hybridization. However, one disadvantage to its widespread use is the high cost of professional paraffin tissue punches, and the complexity in the development of homemade devices previously described in other studies. This study describes a step by step process to develop four different home-made devices made with materials that are common in hospitals and offices. These devices are useful in Histopathology laboratories to obtain paraffin blocks with until 360 samples of tissue, investing from two to fifteen dollars in the development of each device described. 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14860/1/538.pdf http://eprints.uanl.mx/14860/1.haspreviewThumbnailVersion/538.pdf García Garza, Rubén y Rodríguez Vidales, Edgar Paolo y Soto Domínguez, Adolfo (2013) Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays. International Journal of Morphology, 31 (1). pp. 50-54. ISSN 0717-9502 http://doi.org/10.4067/S0717-95022013000100007 doi:10.4067/S0717-95022013000100007 |
| spellingShingle | García Garza, Rubén Rodríguez Vidales, Edgar Paolo Soto Domínguez, Adolfo Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| title_full | Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| title_fullStr | Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| title_full_unstemmed | Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| title_short | Quick and Inexpensive Method to Elaborate Tissue Punches Useful in Paraffin Tissue Microarrays |
| title_sort | quick and inexpensive method to elaborate tissue punches useful in paraffin tissue microarrays |
| url | http://eprints.uanl.mx/14860/1/538.pdf |
| work_keys_str_mv | AT garciagarzaruben quickandinexpensivemethodtoelaboratetissuepunchesusefulinparaffintissuemicroarrays AT rodriguezvidalesedgarpaolo quickandinexpensivemethodtoelaboratetissuepunchesusefulinparaffintissuemicroarrays AT sotodominguezadolfo quickandinexpensivemethodtoelaboratetissuepunchesusefulinparaffintissuemicroarrays |