Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis
The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this stud...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2013
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| Online Access: | http://eprints.uanl.mx/14858/1/535.pdf |
| _version_ | 1824414166001123328 |
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| author | González Ochoa, Guadalupe Menchaca, Griselda Hernández, Carlos Rodríguez, Cristina Tamez, Reyes Contreras, Juan |
| author_facet | González Ochoa, Guadalupe Menchaca, Griselda Hernández, Carlos Rodríguez, Cristina Tamez, Reyes Contreras, Juan |
| author_sort | González Ochoa, Guadalupe |
| collection | Repositorio Institucional |
| description | The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 14 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor. |
| format | Article |
| id | eprints-14858 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| record_format | eprints |
| spelling | eprints-148582020-09-15T12:38:17Z http://eprints.uanl.mx/14858/ Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis González Ochoa, Guadalupe Menchaca, Griselda Hernández, Carlos Rodríguez, Cristina Tamez, Reyes Contreras, Juan The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 14 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor. 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14858/1/535.pdf http://eprints.uanl.mx/14858/1.haspreviewThumbnailVersion/535.pdf González Ochoa, Guadalupe y Menchaca, Griselda y Hernández, Carlos y Rodríguez, Cristina y Tamez, Reyes y Contreras, Juan (2013) Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis. Viruses, 5 (3). pp. 792-805. ISSN 1999-4915 http://doi.org/10.3390/v5030792 doi:10.3390/v5030792 |
| spellingShingle | González Ochoa, Guadalupe Menchaca, Griselda Hernández, Carlos Rodríguez, Cristina Tamez, Reyes Contreras, Juan Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_full | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_fullStr | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_full_unstemmed | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_short | Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis |
| title_sort | mutation distribution in the nsp4 protein in rotaviruses isolated from mexican children with moderate to severe gastroenteritis |
| url | http://eprints.uanl.mx/14858/1/535.pdf |
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