Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood
Background: Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2013
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| Online Access: | http://eprints.uanl.mx/14852/1/525.PDF |
| _version_ | 1824414164015120384 |
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| author | Mendoza Olazarán, Soraya Sarahí Morfín Otero, Rayo Rodríguez Noriega, Eduardo Llaca Díaz, Jorge Martín Flores Treviño, Samantha Maribel González González, Gloria María Villarreal Treviño, Licet Garza González, Elvira |
| author_facet | Mendoza Olazarán, Soraya Sarahí Morfín Otero, Rayo Rodríguez Noriega, Eduardo Llaca Díaz, Jorge Martín Flores Treviño, Samantha Maribel González González, Gloria María Villarreal Treviño, Licet Garza González, Elvira |
| author_sort | Mendoza Olazarán, Soraya Sarahí |
| collection | Repositorio Institucional |
| description | Background: Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec) type, and genetic relatedness of clinical S. hominis isolates. Methodology: S. hominis blood isolates (n = 21) were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE) and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus. Results: Of the S. hominis isolates screened, 47.6% (10/21) were categorized as strong biofilm producers and 23.8% (5/21) as weak producers. Furthermore, 81% (17/21) of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in .70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0–95%. Among mecA-positive isolates, 14 (82%) harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III. Conclusions: The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor new SCCmec structural elements and might be reservoirs of ccrC1 in addition to ccrAB1 and mec complex A. |
| format | Article |
| id | eprints-14852 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | eprints |
| spelling | eprints-148522024-03-05T16:26:24Z http://eprints.uanl.mx/14852/ Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood Mendoza Olazarán, Soraya Sarahí Morfín Otero, Rayo Rodríguez Noriega, Eduardo Llaca Díaz, Jorge Martín Flores Treviño, Samantha Maribel González González, Gloria María Villarreal Treviño, Licet Garza González, Elvira QR Microbiología Background: Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec) type, and genetic relatedness of clinical S. hominis isolates. Methodology: S. hominis blood isolates (n = 21) were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE) and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus. Results: Of the S. hominis isolates screened, 47.6% (10/21) were categorized as strong biofilm producers and 23.8% (5/21) as weak producers. Furthermore, 81% (17/21) of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in .70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0–95%. Among mecA-positive isolates, 14 (82%) harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III. Conclusions: The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor new SCCmec structural elements and might be reservoirs of ccrC1 in addition to ccrAB1 and mec complex A. Public Library of Science 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14852/1/525.PDF http://eprints.uanl.mx/14852/1.haspreviewThumbnailVersion/525.PDF Mendoza Olazarán, Soraya Sarahí y Morfín Otero, Rayo y Rodríguez Noriega, Eduardo y Llaca Díaz, Jorge Martín y Flores Treviño, Samantha Maribel y González González, Gloria María y Villarreal Treviño, Licet y Garza González, Elvira (2013) Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood. PloS one, 8 (4). e61161. ISSN 1932-6203 http://doi.org/10.1371/journal.pone.0061161 doi:10.1371/journal.pone.0061161 |
| spellingShingle | QR Microbiología Mendoza Olazarán, Soraya Sarahí Morfín Otero, Rayo Rodríguez Noriega, Eduardo Llaca Díaz, Jorge Martín Flores Treviño, Samantha Maribel González González, Gloria María Villarreal Treviño, Licet Garza González, Elvira Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| title_full | Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| title_fullStr | Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| title_full_unstemmed | Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| title_short | Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood |
| title_sort | microbiological and molecular characterization of staphylococcus hominis isolates from blood |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/14852/1/525.PDF |
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