Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting
This article provides flow cytometry information regarding levels of expression for hematopoietic stem cell markers CD34 and CD133 obtained simultaneously of the bone marrow and peripheral blood from recipients of allogeneic and autologous transplants of PB hematoprogenitors for treating hematologic...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2016
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| Acceso en línea: | http://eprints.uanl.mx/14797/1/33.pdf |
| _version_ | 1824414144747536384 |
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| author | Jaime Pérez, José Carlos Villarreal Villarreal, César Daniel Vázquez Garza, Eduardo Méndez Ramírez, Nereida Salazar Riojas, María del Rosario Gómez Almaguer, David |
| author_facet | Jaime Pérez, José Carlos Villarreal Villarreal, César Daniel Vázquez Garza, Eduardo Méndez Ramírez, Nereida Salazar Riojas, María del Rosario Gómez Almaguer, David |
| author_sort | Jaime Pérez, José Carlos |
| collection | Repositorio Institucional |
| description | This article provides flow cytometry information regarding levels of expression for hematopoietic stem cell markers CD34 and CD133 obtained simultaneously of the bone marrow and peripheral blood from recipients of allogeneic and autologous transplants of PB hematoprogenitors for treating hematological malignancies
and who were clinically healthy after Z100 days following the procedure. CD34 and CD133 expression is compared regarding type of transplant (autologous vs. allogeneic) and sample cell source (bone marrow vs. peripheral blood). Patients were conditioned with a reduced-intensity conditioning regimen. Also shown is the flow cytometry analysis of mononuclear cell and lymphocyte populations in the peripheral blood of both types of recipients, as well as the characterization of immune cells, including T lymphocyte antigenic make up markers CD3, CD4 and CD8, B lymphocytes and NK cells, including total NK, bright and dim subtypes in the peripheral blood of both types of recipients. For further information and discussion regarding interpretation and meaning of post-transplant flow cytometry analysis, please refer to the article “Assessment of immune reconstitution status in recipients of a successful hematopoietic stem cell transplant from peripheral blood after reduced intensity conditioning” |
| format | Article |
| id | eprints-14797 |
| institution | UANL |
| language | English |
| publishDate | 2016 |
| record_format | eprints |
| spelling | eprints-147972023-05-11T16:17:41Z http://eprints.uanl.mx/14797/ Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting Jaime Pérez, José Carlos Villarreal Villarreal, César Daniel Vázquez Garza, Eduardo Méndez Ramírez, Nereida Salazar Riojas, María del Rosario Gómez Almaguer, David This article provides flow cytometry information regarding levels of expression for hematopoietic stem cell markers CD34 and CD133 obtained simultaneously of the bone marrow and peripheral blood from recipients of allogeneic and autologous transplants of PB hematoprogenitors for treating hematological malignancies and who were clinically healthy after Z100 days following the procedure. CD34 and CD133 expression is compared regarding type of transplant (autologous vs. allogeneic) and sample cell source (bone marrow vs. peripheral blood). Patients were conditioned with a reduced-intensity conditioning regimen. Also shown is the flow cytometry analysis of mononuclear cell and lymphocyte populations in the peripheral blood of both types of recipients, as well as the characterization of immune cells, including T lymphocyte antigenic make up markers CD3, CD4 and CD8, B lymphocytes and NK cells, including total NK, bright and dim subtypes in the peripheral blood of both types of recipients. For further information and discussion regarding interpretation and meaning of post-transplant flow cytometry analysis, please refer to the article “Assessment of immune reconstitution status in recipients of a successful hematopoietic stem cell transplant from peripheral blood after reduced intensity conditioning” 2016 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14797/1/33.pdf http://eprints.uanl.mx/14797/1.haspreviewThumbnailVersion/33.pdf Jaime Pérez, José Carlos y Villarreal Villarreal, César Daniel y Vázquez Garza, Eduardo y Méndez Ramírez, Nereida y Salazar Riojas, María del Rosario y Gómez Almaguer, David (2016) Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting. Data in Brief, 7. pp. 1151-1155. ISSN 23523409 http://doi.org/10.1016/j.dib.2016.03.078 doi:10.1016/j.dib.2016.03.078 |
| spellingShingle | Jaime Pérez, José Carlos Villarreal Villarreal, César Daniel Vázquez Garza, Eduardo Méndez Ramírez, Nereida Salazar Riojas, María del Rosario Gómez Almaguer, David Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| title_full | Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| title_fullStr | Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| title_full_unstemmed | Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| title_short | Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting |
| title_sort | flow cytometry data analysis of cd34 cd133 stem cells in bone marrow and peripheral blood and t b and nk cells after hematopoietic grafting |
| url | http://eprints.uanl.mx/14797/1/33.pdf |
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