High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity
In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expre...
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| Format: | Article |
| Language: | English |
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2016
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| Online Access: | http://eprints.uanl.mx/14789/1/23.pdf |
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| author | Iglesias Figueroa, Blanca Valdiviezo Godina, Norberto Siqueiros Cendón, Tania Sinagawa García, Sugey Ramona Arévalo Gallegos, Sigifredo Rascón Cruz, Quintín |
| author_facet | Iglesias Figueroa, Blanca Valdiviezo Godina, Norberto Siqueiros Cendón, Tania Sinagawa García, Sugey Ramona Arévalo Gallegos, Sigifredo Rascón Cruz, Quintín |
| author_sort | Iglesias Figueroa, Blanca |
| collection | Repositorio Institucional |
| description | In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach
fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and
characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly. |
| format | Article |
| id | eprints-14789 |
| institution | UANL |
| language | English |
| publishDate | 2016 |
| record_format | eprints |
| spelling | eprints-147892020-06-10T20:08:33Z http://eprints.uanl.mx/14789/ High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity Iglesias Figueroa, Blanca Valdiviezo Godina, Norberto Siqueiros Cendón, Tania Sinagawa García, Sugey Ramona Arévalo Gallegos, Sigifredo Rascón Cruz, Quintín QD Química In this study, bovine lactoferrin (bLf), an iron-binding glycoprotein considered an important nutraceutical protein because of its several properties, was expressed in Pichia pastoris KM71-H under AOX1 promoter control, using pJ902 as the recombinant plasmid. Dot blotting analysis revealed the expression of recombinant bovine lactoferrin (rbLf) in Pichia pastoris. After Bach fermentation and purification by molecular exclusion, we obtained an expression yield of 3.5 g/L of rbLf. rbLf and predominantly pepsin-digested rbLf (rbLfcin) demonstrated antibacterial activity against Escherichia coli (E. coli) BL21DE3, Staphylococcus aureus (S. aureus) FRI137, and, in a smaller percentage, Pseudomonas aeruginosa (Ps. Aeruginosa) ATCC 27833. The successful expression and characterization of functional rbLf expressed in Pichia pastoris opens a prospect for the development of natural antimicrobial agents produced recombinantly. 2016 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14789/1/23.pdf http://eprints.uanl.mx/14789/1.haspreviewThumbnailVersion/23.pdf Iglesias Figueroa, Blanca y Valdiviezo Godina, Norberto y Siqueiros Cendón, Tania y Sinagawa García, Sugey Ramona y Arévalo Gallegos, Sigifredo y Rascón Cruz, Quintín (2016) High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity. International Journal of Molecular Sciences, 17 (6). pp. 1-11. ISSN 1422-0067 http://doi.org/10.3390/ijms17060902 doi:10.3390/ijms17060902 |
| spellingShingle | QD Química Iglesias Figueroa, Blanca Valdiviezo Godina, Norberto Siqueiros Cendón, Tania Sinagawa García, Sugey Ramona Arévalo Gallegos, Sigifredo Rascón Cruz, Quintín High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| title_full | High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| title_fullStr | High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| title_full_unstemmed | High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| title_short | High-Level Expression of Recombinant Bovine Lactoferrin in Pichia pastoris with Antimicrobial Activity |
| title_sort | high level expression of recombinant bovine lactoferrin in pichia pastoris with antimicrobial activity |
| topic | QD Química |
| url | http://eprints.uanl.mx/14789/1/23.pdf |
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