Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico
White spot syndrome is a viral disease affecting wild and farm crustaceans that serve as reservoirs. Previous reports have demonstrated high genomic variation in WSS viruses (WSSV) isolated from distinct geographical regions. In this study, we collected wild shrimps (Litopenaeus stylirostris), crabs...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2013
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/14741/1/484.pdf |
| _version_ | 1824414129138434048 |
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| author | González Galaviz, José Reyes Rodriguez Anaya, Libia Molina Garza, Zinnia Judith Ibarra Gámez, José Cuauhtémoc Galaviz Silva, Lucio |
| author_facet | González Galaviz, José Reyes Rodriguez Anaya, Libia Molina Garza, Zinnia Judith Ibarra Gámez, José Cuauhtémoc Galaviz Silva, Lucio |
| author_sort | González Galaviz, José Reyes |
| collection | Repositorio Institucional |
| description | White spot syndrome is a viral disease affecting wild and farm crustaceans that serve as reservoirs. Previous reports have demonstrated high genomic variation in WSS viruses (WSSV) isolated from distinct geographical regions. In this study, we collected wild shrimps (Litopenaeus stylirostris), crabs (Callinectes arcuatus) and farmed shrimp (L. vannamei) in Sonora, Mexico, between 2008 and 2010. DNA was extracted, and the variable regions and transposase genes were subjected to PCR and sequencing. Compared to strains of WSSV from other sites, Mexican samples exhibited a distinct number of repeat units (RUs) in ORF94, ORF75 and ORF125, which ranged between 1-11, 3-15, and 8-11 RUs respectively, and a unique single nucleotide polymorphism (SNP) at position 48 of ORF94. A total of six Mexican genotypes were found in organism from shrimp farm and natural environment. |
| format | Article |
| id | eprints-14741 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| record_format | eprints |
| spelling | eprints-147412024-03-05T17:40:10Z http://eprints.uanl.mx/14741/ Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico González Galaviz, José Reyes Rodriguez Anaya, Libia Molina Garza, Zinnia Judith Ibarra Gámez, José Cuauhtémoc Galaviz Silva, Lucio RB Patología White spot syndrome is a viral disease affecting wild and farm crustaceans that serve as reservoirs. Previous reports have demonstrated high genomic variation in WSS viruses (WSSV) isolated from distinct geographical regions. In this study, we collected wild shrimps (Litopenaeus stylirostris), crabs (Callinectes arcuatus) and farmed shrimp (L. vannamei) in Sonora, Mexico, between 2008 and 2010. DNA was extracted, and the variable regions and transposase genes were subjected to PCR and sequencing. Compared to strains of WSSV from other sites, Mexican samples exhibited a distinct number of repeat units (RUs) in ORF94, ORF75 and ORF125, which ranged between 1-11, 3-15, and 8-11 RUs respectively, and a unique single nucleotide polymorphism (SNP) at position 48 of ORF94. A total of six Mexican genotypes were found in organism from shrimp farm and natural environment. 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14741/1/484.pdf http://eprints.uanl.mx/14741/1.haspreviewThumbnailVersion/484.pdf González Galaviz, José Reyes y Rodriguez Anaya, Libia y Molina Garza, Zinnia Judith y Ibarra Gámez, José Cuauhtémoc y Galaviz Silva, Lucio (2013) Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico. Archives of biological sciences, 65 (3). pp. 945-947. ISSN 0354-4664 http://doi.org/10.2298/ABS1303945G doi:10.2298/ABS1303945G |
| spellingShingle | RB Patología González Galaviz, José Reyes Rodriguez Anaya, Libia Molina Garza, Zinnia Judith Ibarra Gámez, José Cuauhtémoc Galaviz Silva, Lucio Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| title_full | Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| title_fullStr | Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| title_full_unstemmed | Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| title_short | Genotyping of white spot syndrome virus on wild and farm crustaceans from Sonora, Mexico |
| title_sort | genotyping of white spot syndrome virus on wild and farm crustaceans from sonora mexico |
| topic | RB Patología |
| url | http://eprints.uanl.mx/14741/1/484.pdf |
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