Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis
Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequenc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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2013
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| Online Access: | http://eprints.uanl.mx/14691/1/430.PDF |
| _version_ | 1824414114310520832 |
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| author | Gordon, Stephen V. Vera Cabrera, Lucio Ortiz López, Rocío Elizondo González, Ramiro Ocampo Candiani, Jorge |
| author_facet | Gordon, Stephen V. Vera Cabrera, Lucio Ortiz López, Rocío Elizondo González, Ramiro Ocampo Candiani, Jorge |
| author_sort | Gordon, Stephen V. |
| collection | Repositorio Institucional |
| description | Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequencing, we determined the complete genomic nucleotide sequence of the bacteria. According to our data, the genome is a circular chromosome 9,436,348-bp long with 68% G+C content that encodes 8,414 proteins. We observed orthologs for virulence factors, a higher number of genes involved in lipid biosynthesis and catabolism, and gene clusters for the synthesis of bioactive compounds, such as antibiotics, terpenes, and polyketides. An in silico analysis of the sequence supports the conclusion that the bacteria acquired diverse genes by horizontal transfer from other soil bacteria, even from eukaryotic organisms. The genome composition reflects the evolution of bacteria via the acquisition of a large amount of DNA, which allows it to survive in new ecological niches, including humans. |
| format | Article |
| id | eprints-14691 |
| institution | UANL |
| language | English |
| publishDate | 2013 |
| record_format | eprints |
| spelling | eprints-146912021-10-19T20:58:39Z http://eprints.uanl.mx/14691/ Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis Gordon, Stephen V. Vera Cabrera, Lucio Ortiz López, Rocío Elizondo González, Ramiro Ocampo Candiani, Jorge Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequencing, we determined the complete genomic nucleotide sequence of the bacteria. According to our data, the genome is a circular chromosome 9,436,348-bp long with 68% G+C content that encodes 8,414 proteins. We observed orthologs for virulence factors, a higher number of genes involved in lipid biosynthesis and catabolism, and gene clusters for the synthesis of bioactive compounds, such as antibiotics, terpenes, and polyketides. An in silico analysis of the sequence supports the conclusion that the bacteria acquired diverse genes by horizontal transfer from other soil bacteria, even from eukaryotic organisms. The genome composition reflects the evolution of bacteria via the acquisition of a large amount of DNA, which allows it to survive in new ecological niches, including humans. 2013 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14691/1/430.PDF http://eprints.uanl.mx/14691/1.haspreviewThumbnailVersion/430.PDF Gordon, Stephen V. y Vera Cabrera, Lucio y Ortiz López, Rocío y Elizondo González, Ramiro y Ocampo Candiani, Jorge (2013) Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis. PLoS ONE, 8 (6). e65425. ISSN 1932-6203 http://doi.org/10.1371/journal.pone.0065425 doi:10.1371/journal.pone.0065425 |
| spellingShingle | Gordon, Stephen V. Vera Cabrera, Lucio Ortiz López, Rocío Elizondo González, Ramiro Ocampo Candiani, Jorge Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| title_full | Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| title_fullStr | Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| title_full_unstemmed | Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| title_short | Complete Genome Sequence Analysis of Nocardia brasiliensis HUJEG-1 Reveals a Saprobic Lifestyle and the Genes Needed for Human Pathogenesis |
| title_sort | complete genome sequence analysis of nocardia brasiliensis hujeg 1 reveals a saprobic lifestyle and the genes needed for human pathogenesis |
| url | http://eprints.uanl.mx/14691/1/430.PDF |
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