Expression of the HPV18/E6 oncoprotein induces DNA damage
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genom...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://eprints.uanl.mx/14581/1/44.pdf |
| _version_ | 1824414087189102592 |
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| author | Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime |
| author_facet | Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime |
| author_sort | Cortés Gutiérrez, Elva Irene |
| collection | Repositorio Institucional |
| description | This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique
and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using
image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene
showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration. |
| format | Article |
| id | eprints-14581 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-145812021-07-13T15:35:44Z http://eprints.uanl.mx/14581/ Expression of the HPV18/E6 oncoprotein induces DNA damage Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime QR Microbiología This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration. 2017 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14581/1/44.pdf http://eprints.uanl.mx/14581/1.haspreviewThumbnailVersion/44.pdf Cortés Gutiérrez, Elva Irene y García Vielma, Catalina y Aguilar Lemarroy, Adriana y Vallejo Ruíz, Veronica y Piña Sánchez, Patricia y Zapata Benavides, Pablo y Gosálvez, Jaime (2017) Expression of the HPV18/E6 oncoprotein induces DNA damage. European Journal of Histochemistry, 61 (2). pp. 96-101. ISSN 1121-760X http://doi.org/10.4081/ejh.2017.2773 doi:10.4081/ejh.2017.2773 |
| spellingShingle | QR Microbiología Cortés Gutiérrez, Elva Irene García Vielma, Catalina Aguilar Lemarroy, Adriana Vallejo Ruíz, Veronica Piña Sánchez, Patricia Zapata Benavides, Pablo Gosálvez, Jaime Expression of the HPV18/E6 oncoprotein induces DNA damage |
| thumbnail | https://rediab.uanl.mx/themes/sandal5/images/online.png |
| title | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_fullStr | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full_unstemmed | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_short | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_sort | expression of the hpv18 e6 oncoprotein induces dna damage |
| topic | QR Microbiología |
| url | http://eprints.uanl.mx/14581/1/44.pdf |
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