Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments
Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawbac...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2017
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| Acceso en línea: | http://eprints.uanl.mx/14487/1/10.pdf |
| _version_ | 1823686990454849536 |
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| author | Garza Cervantes, Javier Alberto Chávez Reyes, Arturo Castillo, Elena C. García Rivas, Gerardo Ortega Rivera, Oscar Antonio Salinas, Eva Ortiz Martínez, Margarita Gómez Flores, Sara Leticia Peña Martínez, Jorge Arturo Pepi Molina, Alan Treviño Gonzalez, Mario Tadeo Zárate Kalfópulos, Xristo Cantú Cárdenas, María Elena Escárcega González, Carlos Enrique Morones Ramírez, José Rubén |
| author_facet | Garza Cervantes, Javier Alberto Chávez Reyes, Arturo Castillo, Elena C. García Rivas, Gerardo Ortega Rivera, Oscar Antonio Salinas, Eva Ortiz Martínez, Margarita Gómez Flores, Sara Leticia Peña Martínez, Jorge Arturo Pepi Molina, Alan Treviño Gonzalez, Mario Tadeo Zárate Kalfópulos, Xristo Cantú Cárdenas, María Elena Escárcega González, Carlos Enrique Morones Ramírez, José Rubén |
| author_sort | Garza Cervantes, Javier Alberto |
| collection | Repositorio Institucional |
| description | Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become
a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and
significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawback of
transition metals is their toxicity in eukaryotic cells. Here, we show that the barriers to use them as
therapeutic agents could be mitigated by combining them with silver. We demonstrate that synergism
of combinatorial treatments (Silver/transition metals, including Zn, Co, Cd, Ni, and Cu) increases up
to 8-fold their antimicrobial effect, when compared to their individual effects, against E. coli and B.
subtilis. We find that most combinatorial treatments exhibit synergistic antimicrobial effects at low/
non-toxic concentrations to human keratinocyte cells, blast and melanoma rat cell lines. Moreover, we
show that silver/(Cu, Ni, and Zn) increase prokaryotic cell permeability at sub-inhibitory concentrations,
demonstrating this to be a possible mechanism of the synergistic behavior. Together, these results
suggest that these combinatorial treatments will play an important role in the future development of
antimicrobial agents and treatments against infections. In specific, the cytotoxicity experiments show
that the combinations have great potential in the treatment of topical infections. |
| format | Article |
| id | eprints-14487 |
| institution | UANL |
| language | English |
| publishDate | 2017 |
| record_format | eprints |
| spelling | eprints-144872024-03-06T18:46:21Z http://eprints.uanl.mx/14487/ Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments Garza Cervantes, Javier Alberto Chávez Reyes, Arturo Castillo, Elena C. García Rivas, Gerardo Ortega Rivera, Oscar Antonio Salinas, Eva Ortiz Martínez, Margarita Gómez Flores, Sara Leticia Peña Martínez, Jorge Arturo Pepi Molina, Alan Treviño Gonzalez, Mario Tadeo Zárate Kalfópulos, Xristo Cantú Cárdenas, María Elena Escárcega González, Carlos Enrique Morones Ramírez, José Rubén Due to the emergence of multi-drug resistant strains, development of novel antibiotics has become a critical issue. One promising approach is the use of transition metals, since they exhibit rapid and significant toxicity, at low concentrations, in prokaryotic cells. Nevertheless, one main drawback of transition metals is their toxicity in eukaryotic cells. Here, we show that the barriers to use them as therapeutic agents could be mitigated by combining them with silver. We demonstrate that synergism of combinatorial treatments (Silver/transition metals, including Zn, Co, Cd, Ni, and Cu) increases up to 8-fold their antimicrobial effect, when compared to their individual effects, against E. coli and B. subtilis. We find that most combinatorial treatments exhibit synergistic antimicrobial effects at low/ non-toxic concentrations to human keratinocyte cells, blast and melanoma rat cell lines. Moreover, we show that silver/(Cu, Ni, and Zn) increase prokaryotic cell permeability at sub-inhibitory concentrations, demonstrating this to be a possible mechanism of the synergistic behavior. Together, these results suggest that these combinatorial treatments will play an important role in the future development of antimicrobial agents and treatments against infections. In specific, the cytotoxicity experiments show that the combinations have great potential in the treatment of topical infections. 2017 Article PeerReviewed text en cc_by_nc_nd http://eprints.uanl.mx/14487/1/10.pdf http://eprints.uanl.mx/14487/1.haspreviewThumbnailVersion/10.pdf Garza Cervantes, Javier Alberto y Chávez Reyes, Arturo y Castillo, Elena C. y García Rivas, Gerardo y Ortega Rivera, Oscar Antonio y Salinas, Eva y Ortiz Martínez, Margarita y Gómez Flores, Sara Leticia y Peña Martínez, Jorge Arturo y Pepi Molina, Alan y Treviño Gonzalez, Mario Tadeo y Zárate Kalfópulos, Xristo y Cantú Cárdenas, María Elena y Escárcega González, Carlos Enrique y Morones Ramírez, José Rubén (2017) Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments. Scientific reports, 7 (1). pp. 1-16. ISSN 2045-2322 http://doi.org/10.1038/s41598-017-01017-7 doi:10.1038/s41598-017-01017-7 |
| spellingShingle | Garza Cervantes, Javier Alberto Chávez Reyes, Arturo Castillo, Elena C. García Rivas, Gerardo Ortega Rivera, Oscar Antonio Salinas, Eva Ortiz Martínez, Margarita Gómez Flores, Sara Leticia Peña Martínez, Jorge Arturo Pepi Molina, Alan Treviño Gonzalez, Mario Tadeo Zárate Kalfópulos, Xristo Cantú Cárdenas, María Elena Escárcega González, Carlos Enrique Morones Ramírez, José Rubén Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| thumbnail | http://148.234.3.147/themes/sandal5/images/online.png |
| title | Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| title_full | Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| title_fullStr | Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| title_full_unstemmed | Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| title_short | Synergistic Antimicrobial Effects of Silver/Transition-metal Combinatorial Treatments |
| title_sort | synergistic antimicrobial effects of silver transition metal combinatorial treatments |
| url | http://eprints.uanl.mx/14487/1/10.pdf |
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